Bacterial Toxin Research Citations

… FoxP3DTR mice to receive transferred Treg cells were treated with 50 mg/kg diphtheria toxin (DT) (List Labs, 150) starting from 5 days prior to tumor inoculation and for every 3 days until time of euthanization. Mice were monitored daily for tumor growth. Tumor volume was measured through digital calipers along the x, y, and z axes and calculated as (xyz)/2. Mice were to be euthanized if tumors exceeded size limit agreed by the Institutional Review Board (IRB), however no animal reached this volume during the duration of the experiment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… depletion was performed in CD169DTR/+ mice upon i.p. injection with 10 µg/kg DT (List Biological, Lot #15043A1) in 100 µL PBS. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… DTX List Biologicals …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

2.5 Construction of lipodystrophy mouse model and ibuprofen treatment

… To induce deletion of Cre-positive cells, mice were injected daily with 100 ng/mouse/day of diphtheria toxin (DT) (Biological List Laboratories, Cat#150) for 5 days via the intraperitoneal (i.p.) route. The R26-(SA-LSL-DTR) flox/flox mice received an equal amount of PBS as the control group. Samples were collected at day 7 after the first injection (Figure 2E). To determine the effect of ibuprofen on lipodystrophy metabolic dysfunction, 1 mg/mL ibuprofen (Sigma, St. Louis, I1892) was continuously added to the drinking water of both Con + IBU and DTRADQ + IBU groups of mice 7 days prior to intraperitoneal injection until the end of the experiment. After 7 days of IBU pretreatment (D−7 to D0), mice in both the DTRADQ + water and DTRADQ + IBU groups were intraperitoneally injected with DT daily for 5 consecutive days (D0 to D4), and at the same time, mice in the Con + water and Con + IBU groups were injected with an equal volume of PBS. All mice were sacrificed to harvest for tissues on D6 (Figure 4A).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Animal husbandry and procedures

… Diphtheria Toxin (List Labs, #150) was dissolved in PBS and administered to postnatal pups by intraperitoneal injection at P0, P2, and P4 at 5ng/g of body weight. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria toxin treatment and adoptive transfer of Treg cells to Foxp3DTR mice

For the Treg cell depletion studies, Foxp3DTR mice were intra-peritoneally injected with a diphtheria toxin (DT, List Biologicals cat. no. 150) loading dose (50 μg/kg) on day 14 post-influenza virus inoculation and given three maintenance doses (10 μg/kg) every other day thereafter to deplete endogenous Treg cells. For the adoptive transfer experiments, Foxp3DTR mice received a DT loading dose one day before influenza virus inoculation as well as 1 x 106 Treg cells via retro-orbital adoptive transfer from either Black 6 or TBET KO mice enriched using the Miltenyi Biotec CD4+ CD25+ Regulatory T Cell Isolation Kit (cat. no. 130-091-041) according to the manufacturer’s instructions. Foxp3DTR mice receiving adoptive transfers were then given a DT maintenance dose every other day until tissue harvest.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Animals and Genotyping

In this paper, the different mice including wild-type C57BL/6J mice, transgene Lgr5-EGFPcreERT2 mice (Jackson Laboratory), Sox9 creERT2 mice (Jackson Laboratory), and Rosa26-CAG-tdTomato mice (Jackson Laboratory) were used. In P1-2 mice, the Cre recombination enzyme was activated by intraperitoneal injection of tamoxifen (Sigma, T5648), and the dose was controlled at 0.3 mg/4 g. Diptheria toxin (List Biologicals Lab) was intraperitoneally injected into P4 mice at a dose of 10 ng g−1. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Diphtheria Toxin List Labs 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… To deplete Foxp3+ Treg in Foxp3-DTR mice, DT (List Biological Laboratories, California, USA) 500 ng per mouse was injected intraperitoneally (i.p.) on days four and six after the start of MC903 treatment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Tamoxifen and diphtheria toxin treatment

Tamoxifen (MedChemExpress) was dissolved in corn oil (20 mg ml−1) and heated at 37°C for 3 hours. To activate Cx3cr1CreERT2-mediated recombinase, each mouse was injected with Tamoxifen (100 mg/kg body weight) by oral gavage every three days, starting from Day 12 post-intraportal injection, as indicated in Fig. 2e. For proliferation tracing experiment, DreERT2-mediated recombinase was introduced by a single dose of tamoxifen gavage (100 mg/kg body weight) on Day 12 post-intraportal injection, as indicated in Fig. 3f. For KC specific depletion, DreERT2-mediated recombinase was introduced by 8 doses of tamoxifen gavage (2 mg per dose), as indicated in Fig. 5d. A single dose of 200 ng DT (List Laboratories) was intraperitoneally introduced one week after the last Tamoxifen injection. The cancer cells were inoculated into liver through portal vein one day after DT injection.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae