Bacterial Toxin Research Citations

– … We gave 49 postnatal day (P)2 mice a systemic injection of diphtheria toxin (DT; 4 ng/g im List Biological Laboratories, Inc.) (Figure 2.1). Pups were raised with their mothers until weaning at P21. After weaning, pups were group housed in same sex, mixed genotype …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… littermates that successfully entered and survived status epilepticus were selected to receive either DT (List Biological Laboratories, Product No: 150, 40 g/kg/dose intraperitoneal) or saline treatment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diptheria toxin (DT) (1.0mg) (List Biological Laboratories, Campbell, CA) was reconstituted with 1.0ml sterile distilled water and then diluted to 2ng/ml in sterile PBS. DT (20ng/g i.p) was administered to mice to induce monocyte/ M depletion in CD11bDTR transgenic mice and FVB strain controls 24h prior to experiments.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials:

…while ETA, DT, and CT were purchased from List Biologicals (Campbell, CA). …

CT toxicity assay:

CHO-K1 cells (ATCC #CCL-61) were co-incubated with a combination of CT and grape compound for 18 h before cAMP levels were quantified as previously described…

Vero-d2EGFP toxicity assay:

Ricin, ETA, DT, and ST1/ST2 intoxication assays were peformed with Vero-d2EGFP cells as previously described…

Author did not specify which Cholera toxin was utilized.  List Labs provides Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae and Product #101 – Cholera Toxin from Vibrio cholerae.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
• Product #160 – Exotoxin A from Pseudomonas aeruginosa

Adoptive transfer and infection:

Spleens were collected from naive CD45.1+ gBT-I TCR transgenic mice, and CD8 T cells were magnetically purified by CD8 microbeads or …For experiments with DT depletion, animals were injected intraperitoneally with 0.5g of DT (List Biological) 1 day before WT HSV-2 challenge¹³.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Pilocarpine and DT treatment:

At 8 weeks of age (1 week after the last tamoxifen injection), mice were injected intraperitoneally with 1 mg/kg methyl scopolamine nitrate… NestinCreER^T2::DTr^+/::GFP^+/ and NestinCreER^T2::DTr^/::GFP^+/ littermates that successfully entered and survived status epilepticus were selected to receive either DT (List Biological Laboratories, catalog #150, 40 g/kg, i.p.) or saline treatment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Cell Depletion:

For in vivo cell depletion, DT (0.5 mg/mouse/dose, List Biological Laboratories) was given intraperitoneally every 3 days for four times, then mice were sacrificed on day 10. In HFD-fed animals, DT was given in the last 10 days of the indicated HFD feeding period.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

In vivo depletion of Langerin⁺ cells:

Langerin-eGFP-DTR mice were injected i.p. with DT (1 g in 200 l saline; List Biological Laboratories). 24 h after DT injection, epidermis and dermis were separated by treatment of dispase II (1 mg/ml; Gibco) overnight at 4C, and LC depletion in the epidermis was measured by FACS analysis.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Depletion of regulatory T cells in DEREG mice:

For conditional ablation of Tregs, DEREG mice were injected intraperitoneally [ip] twice with 1 g of diphtheria toxin [DT] [List Biological Laboratories, Campbell, CA, USA] 1 day before and on the first day of DSS treatment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Animals:

Female Foxd1^+/Cre (20) mice were bred with Gt(ROSA)26Sor^{tm1(HBEGF)Awai} mice to generate mice expressing the simian DT receptor (DTR) in FoxD1⁺ progenitor cells and their descendants (Foxd1Cre::RsDTR) in development and littermate controls (RsDTR). In some experiments, female Foxd1^+/Cre mice were crossed with Rosa26^{+/(STOP)-tdTomato} [B6;129S6-Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}] mice. To ablate pericytes, 25 ng/g DT (List Biological Laboratories) was injected intraperitoneally into Foxd1Cre::RsDTR mice or littermate controls. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae