Bacterial Toxin Research Citations

Mouse EAE induction and CNS lymphocyte isolation

Mice were immunized s.c. with 100 μl of emulsified IFA supplemented with 500 μg Mycobacterium tuberculosis H37Ra (DIFCO) and 100 μg of MOG35–55, and received an i.p. injection of 200 ng pertussis toxin (List Biological Laboratories) at the time of immunization as well as 48 h later. The mice were observed daily for clinical signs and scored …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Diphtheria toxin treatment:

Diphtheria toxin (DT) was administered to DTR mice, genetically engineered to express the human DTR selectively in hair cells (Tong et al. 2015). A single 25 g/kg dose of DT (List Biological Laboratories, no. 150) was delivered via intramuscular injection to 4-wk-old DTR mice. Within 6 days after DT injection, DTR mice lose all of their hair cells and are completely deaf (Tong et al. 2015). After DT injection, DTR mice were allowed to survive for 2 wk before tissue collection.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Tracer experiments

Cholera toxin subunit injections:

…Briefly, the retrograde tracer cholera toxin subunit (CTB; List Biological Laboratories, Campbell, CA) was targeted to the PVN (n = 10) and to the PIL (n = 15) in lactating mother rats at postpartum day 4. …Glass micropipettes of 15 to 20 µm internal diameter were filled with 0.25% CTB dissolved in PB and lowered to the following stereotaxic coordinates (35): anteroposterior (AP), 1.7 mm; lateral (L), 0.4 mm; ventral (V), 7.4 mm for the PVN; and AP, 5.2 mm; L, 2.6 mm; and V, 6.4 mm for the PIL. CTB was applied by iontophoresis using a constant current source…

Visualization of CTB:

Sections were pretreated in PB-containing 0.5% Triton X-100 and 3% bovine serum albumin for 1 hour. Sections were then incubated in goat anti-CTB antiserum (1:10,000; #703, List Biological Laboratories; …

Double immunolabeling of CTB and TIP39 or CB:

Every fourth free-floating section was first stained for TIP39 by using FITC-tyramide amplification fluorescent immunocytochemistry, as described earlier. Sections were then incubated overnight in goat anti-CTB or …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

– … A borosilicate glass micropipette … backfilled with low-salt cholera toxin b subunit (CTb; 0.5%; List Biological Laboratories; Luppi et al., 1990) was lowered with hydraulic micropositioner (David Kopf Instruments) into the gigantocellular reticular …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Reagents:

… Botulinum toxins A and B were purchased from List Biologicals Laboratories, Campbell, CA. …

Study of the paralytic effect of BoNTs on detrusor contractions:

After intensive washings and after at least 20 min of stable contractions, BoNTs were added to the baths, with final concentrations of 0.1 to 5 nmol/L. Each strip was exposed to only one concentration of BoNT. Amplitude of contractions was measured until 90% of the signal was abolished, or for 4 h if this value was not reached (for low concentrations). …

ELISA:

BMDMs were primed with ultra-pure LPS (3 ng/mL; List Biological Lab) for 4 hr and then stimulated with particles for 4 hr at 37C. …

Cell Death Assay:

LPS (3 ng/mL)-primed BMDMs were stimulated with the indicated particles for 4 hr at 37C. …

Immunoblot Analysis:

…In brief, BMDMs (1  10⁶/well) were seeded in 6-well plates and were cultured overnight. Cells were primed with LPS (3 ng/mL) for 4 hr at 37C. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Induction and Scoring of EAE:

EAE was induced as in prior studies… In addition, each animal received 200ng pertussis toxin (List Biological, Campbell, CA, USA) in 0.1ml PBS by i.p. injection at 0h and 48h post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Sorting and activation of BMBs and SPBs:

…After sorting, 0.510⁵ BMBs and SPBs were activated with IgE…or CT (100ng/ml, List Biological Laboratories, Campbell, CA) for 2hours. …

Immunization with CTO:

For immunization, Mcpt8-cre-YFP mice and Mcpt8-creROSA DT mice were challenged intranasally with 40g of OVA_323-339 peptide with 2µg of CT (List Biological laboratories) in PBS³². …

Experimental autoimmune encephalomyelitis:

…All mice also received 200ng pertussis toxin (List Biological Laboratories) intraperitoneally on day 0 and 2. Mice were measured clinical scores for EAE according to the following scale: …

Adoptive transfer of BMBs:

After BM culture, WT and IL-6 deficient CD49b(DX5)⁺/c-kit basophils were isolated using a FACSAria II. Isolated basophils were injected intravenously into IL-6 deficient mice. The next day, mice were challenged intranasally with 40g of OVA peptides with 2µg of CT. Inflamed lungs and draining LNs were collected after 7 days.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

ELISA assays:

An ELISA assay was used to measure the serum IgG titer of immunized animals for TcdA and TcdB. …96-well plates were coated with 50 ng per well TcdA (List Biological Laboratories, Campbell, CA), 25 ng per well TcdB (List Biological Laboratories, Campbell, CA), 250 ng per well 1mCDTa_ec or 250 ng per well proCDTb_ec in PBS and incubated overnight at 4C. …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

Immunization of Mice:

…Mice received 200ng/mouse pertussis toxin (List Biological Laboratories Inc., Campbell, CA, USA) i.p. on the day of immunization and 2 days thereafter. Clinical signs of disease were scored daily according to a standard scoring system (0, no clinical signs; 1, loss of tail tone; 2, hind limb weakness; 3, complete hind limb paralysis; 4, hind limb and forelimb paralysis; and 5, moribund or dead). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)