Bacterial Toxin Research Citations

Materials:

Staphylococcus aureus enterotoxin B (SEB, List Biological laboratories, INC, CA) were utilized to induce nasal polyp in sino-nasal cavity.

Animal Nasal Polyp Model:

…We prepared 5 different treatment formulations: PBS vehicle without RSV, a bolus RSV solution, and 3 distinct suspensions of PLGA/PEG NM in PBS. The formulations were intranasally administered 1 day after the OVA and SEB administration, which were scheduled 3 times per week. At each administration, 20l of the same formulation was applied to both nostrils; hence, a total of 40l was administered to each mouse. …

…On day 1, each mouse was injected subcutaneously with 10 µl of MOG emulsion and injected intraperitoneally with 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) diluted in sterile PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice and immunizations:

TNP‐Ficoll (Biosearch Technologies; 10 μg/mouse in PBS) and NP‐CGG (Biosearch Technologies; 10 μg/mouse in PBS:Alum (Thermo Scientific) 3:1) immunizations were done by intra‐peritoneal (i.p.) injection. For cholera toxin (Ctx; List Biological Laboratories, Inc.) immunization, mice were starved for 2 h and HBSS + 7.5% sodium bicarbonate solution (4:1 ratio) was administered by intra‐gastric (i.g.) gavage, followed 30 min later by i.g. gavage of 2.5 μg active Ctx in PBS. Mice were culled at d14 post‐immunization for analysis. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE:

Along with immunization and on day 2 postimmunization, 200 ng of pertussis toxin (List Biological Laboratories) or …was administered by intraperitoneal injection, when indicated.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

For EAE induction incomplete Freunds adjuvant (IFA) and killed Mycobacterium tuberculosis strain H37RA were purchased from Difco (Sparks, MD), and pertussis toxin was purchased from List Biological Laboratories (Campbell, CA).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Injection of purified toxins:

HIOs were prepared and injected as described above. For these
experiments purified C. difficile toxin A (TcdA) or B (TcdB) from strain VPI 10463
were purchased (List Biological Laboratories cat# 152C or#155D), reconstituted
at a concentration of 1g/L, aliquoted and stored a -80C. Due to lot-to-lot
variation, each new lot was tested for activity using the Vero cell cytotoxicity
assay. Each HIO was injected with approximately 2L of either 12.8ng/L TcdA
or 25.6ng/L TcdB with 1mM FD4. For each experiment, new aliquot of toxin was
thawed and used.

• Product #152C  – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

– … Male C3H/HeOuJ mice were intragastrically (ig) sensitized with 20 mg whey (DMV International, Veghel, The Netherlands), with 10 g cholera toxin (CT, List Biological Laboratories, Campbell, CA, USA) as an adjuvant …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reagents:

…goat anti-cholera toxin B subunit (#703; List Biological Laboratories, Campbell, CA; 1:5000), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Fifty microliters of whole blood was added to a tube containing 500 pg/mL Escherichia coli LPS (List Biological Laboratories, Campbell, California) within 60 minutes of blood collection. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Ex vivo blood stimulation assays:

…Escherichia coli O111:B4 LPS 10 ng/mL (List Biological Laboratories, Campbell, CA, USA) and Salmonella minnesota Re595 LPS 10 ng/mL (List Biological Laboratories). Plates were placed on a shaker at 37C under 5% CO₂ for 6 hours before being spun down; the plasma supernatant was removed and frozen at 80C. Monocyte counts were performed in the hospital clinical laboratory.

• Product #201 – LPS from Escherichia coli O111:B4
• Product #304  – LPS from Salmonella minnesota R595 (Re)