Bacterial Toxin Research Citations

… Female backcross mice, 4-6 weeks of age, were sensitized to peanut by oral gavage with peanut extract plus cholera toxin (List Biological Labs, Campbell, CA) once per …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… For retrograde tracing, a combinatorial approach with partial overlap was used for immunohistochemistry, so that TH and CTB
immunoreactivity was tested in all samples, but different series from the same animal could be tested for three additional markers.
A number of markers have been identified as being selectively expressed in populations of dopamine neurons21–26,46,68–70; we therefore selected the three proteins (Sox6, Aldh1a1, and calbindin) that show good population discrimination and can be reliably detected
using immunohistochemistry. Sections were incubated overnight at room temperature in PBS-Triton with chicken anti-TH (1:250/
500, ab76442, Abcam), mouse anti-CTB (1:500, ab35988, Abcam) or goat anti-CTB (1:5000), #703, List Biological Labs), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Cell culture and stimulation

… For stimulation, Raw264 cells were overlaid with fresh DMEM/10% FBS supplemented with the following ligands: 10 or 100 ng/ml smooth LPS from Escherichia coli O111:B4 (List Biological Laboratories), …

• Product #201 – LPS from Escherichia coli O111:B4

Injection of neural tracer into major pelvic ganglia (MPG)

The paired MPGs are located on the surface of the dorsolateral lobe of the prostate (male) or uterine cervix (female). Spinal cord neurons projecting to the MPG were identified by retrograde neural tracing with cholera toxin subunit B (CTB). Six rats (three male and three female) were anesthetized with isoflurane (3% in oxygen for induction, 1–2% for maintenance), followed by a midline ventral incision to access the MPGs. Each MPG was separated from underlying tissue by blunt dissection with fine angled forceps, and a sterile 2 mm square piece of parafilm inserted underneath to minimize leakage of tracer. A solution of CTB (low-salt, 0.3% w/v; List Biological Labs, CA, USA) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

4.2. Air Pouch Model

To evaluate the possible immunomodulatory effects of PHA 568487 in vivo, the air pouch model of acute inflammation was used [12,25]. At 8 weeks of age, male C57BL/6JRj mice (Janvier Labs, Le Genest-Saint-Isle, France) were injected with 3 mL of sterile air between the scapulae under isoflurane anesthesia. Three days later, the air pouch was re-filled with 3 mL of sterile air. On the sixth day, the mice received an intraperitoneal injection of either PBS (Thermo Fisher Scientific, Waltham, MA, USA) or PHA 568487 (Tocris Bioscience, Ellisville, MO, USA) at 12.4 μmol kg−1 (5 mg/kg) or 123.4 μmol kg−1 (50 mg/kg). Fifteen minutes later, all mice were injected with LPS (10 μg, List Biological Laboratories Inc., Campbell, CA, USA) directly into the pouch. The mice were sacrificed six hours after the LPS injection by an overdose of pentobarbital (Apoteket AB, Stockholm, Sweden). …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://www.listlabs.com/product-information/lipopolysaccharides/

Experimental autoimmune encephalomyelitis

As previously described,33,34 experimental autoimmune encephalomyelitis (EAE) was induced in 8-10-week-old female C57BL-6J
mice by subcutaneous (s.c.) injection of 200 mg MOG35-55 peptide (Genemed Synthesis, San Antonio, TX) emulsified in complete
Freund’s Adjuvant (CFA) containing 0.2 mg Mycobacterium tuberculosis H37Ra into the flanks of both hindlimbs. CFA control animals
received s.c. injections without MOG35-55 peptide. On the day of immunization and two days later, all mice were also administered
300 ng of pertussis toxin (List biological laboratories, Campbell, CA) intraperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Diphtheria Toxin (DT) List Labs Cat#150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… along with biotin-conjugated Cholera toxin B subunit (1:500, List Labs, Cat# 112). …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

5.16 EAE induction

… For this experiment, we used Swiss Jim Lambert (SJL) female mice that were 10–15 weeks old (Janvier, SAS, Le Genest Saint Isle, France). The mice were housed under standard conditions with a 12:12 hours light–dark-cycle and ad libitum access to food and water. … To induce EAE, the animals were immunized with 250 μg of proteolipid peptide PLP139–151 (The peptide was produced by Peptide synthesis group at Charité), emulsified in 100 μL complete Freund’s adjuvant (Cat # 9225949; Thermo Fisher Scientific, Waltham, MA, USA) and 800 μg Mycobacterium tuberculosis H37Ra (Cat # 231141; Difco, Detroit, MI, USA). Furthermore, 250 ng of pertussis toxin (Cat # LT-0105; List Biological Laboratories, Campbell, CA, USA) resuspended in PBS (Gibco, Grand Island, NY, USA) was injected intraperitoneally on day 0 and day 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Autoimmune Encephalomyelitis model

Fourteen days after tamoxifen treatment, mice were immunized subcutaneously with 100 μl of emulsified Complete Freund adjuvant (BD Difco) supplemented with 4 mg/ml Mycobaterium tuberculosis H37Ra (BD Difco) and 200 μg MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK, Genemed Synthesis) and received intraperitoneal injections of 200 ng Pertussis Toxin from Bordetella pertussis (List biological Laboratories) at the time of immunization and 48h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)