Bacterial Toxin Research Citations

Induction of relapsing EAE:

Female SJL/J mice (Charles River, Shizuoka, Japan), 810 weeks old, were immunized with the encephalitogenic proteolipid peptide (PLP)139-151 (sequence: HSLGKWLGHPDKF; purity>90%) emulsified in complete Freund’s adjuvant (Difco, Detroit, MI) plus Mycobacterium tuberculosis H37Ra (BD, Sparks, MD) (5 mg/ml). Then, mice were immunized with 0.2 ml of the emulsion injected subcutaneously into two locations at the tail root. At the time of immunization and 48 h later, the mice were administered 400 ng of pertussis toxin intravenously (List Biological Laboratories, Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For other injections, cholera toxin B (List Biological Laboratories, Inc) or latex microspheres (Lumafluor … The CTB polyclonal antibody (List Biologicals, Cat# 703, Lot# 7032A8, AB_10013220) was generated against CTB in goat. …

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE:

…Immediately and 24 h after immunization, mice were administered 200 ng pertussis toxin intraperitoneally (List Biological Labs). At 1 week after induction, EAE mice received a booster of 200 µg of MOG in incomplete Freund’s adjuvant. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell Depletion:

For in vivo cell depletion, DT (0.5 mg/mouse/dose, List Biological Laboratories) was given intraperitoneally every 3 days for four times, then mice were sacrificed on day 10. In HFD-fed animals, DT was given in the last 10 days of the indicated HFD feeding period.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents:

Anthrax lethal toxin, consisting of protective antigen and lethal factor was from List Biological Laboratories.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

EAE:

In 2D2 TCR-Tg mice, EAE was induced with 100 mg MOG3555. At the time of and 2 d after im- munization, mice were injected ip with 300 ng Bordetella pertussis toxin (List Biological Laboratories) in PBS.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In vivo depletion of Langerin⁺ cells:

Langerin-eGFP-DTR mice were injected i.p. with DT (1 g in 200 l saline; List Biological Laboratories). 24 h after DT injection, epidermis and dermis were separated by treatment of dispase II (1 mg/ml; Gibco) overnight at 4C, and LC depletion in the epidermis was measured by FACS analysis.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Mice were rested for two weeks before being sensitized. After the two week rest period, mice were sensitized with 2 mg peanut extract and 10 µg cholera toxin (List Biological Laboratories, Campbell, CA) by oral gavage once a week for three weeks. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

IL-1 signaling assay:

… or Bacillus anthracis lethal factor (100 ng/ml; List Biological Laboratories) was prepared with these liposomes as controls. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Materials: 

…The Anthrax PA measured by circular dichroism was obtained from List Labs (Lot: 17115A6B). …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis