Bacterial Toxin Research Citations

EAE Induction:

Active immunization: mice were immunized subcutaneously…distributed in four sites on the flank. 200 ng pertussis toxin (List Biological Laboratories) was given intraperitoneally on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…The BoNT/B L-chain comprising amino acids 1 to 436 and the C-terminal region of the BoNT/B H-chain comprising amino acids 858 to 1291 (HCc) were obtained from List Biological Laboratories (Campbell, CA, USA). …

• Product #620A – Botulinum Neurotoxin Type B Light Chain, Recombinant
• Product #622A – Botulinum Neurotoxin Type B Heavy Chain, Recombinant, Binding Domain

…ELISAs were performed as described [123]. Briefly, 96-well plates were coated with 1 g/well of recombinant PA, LF (List Biologicals, Campbell, CA), …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Author did not specify which List Labs Lethal Factor was utilized.  List Labs offers Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Anthrax protective antigen IgG and IgE ELISAs:

Ninety-six-well microtiter plates (Corning, Lowell, MA) were coated with 1g/well of recombinant PA (List Biological Laboratories, Campbell, CA). …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

C3H/HeJ mice were rendered peanut allergic by means of oral sensitization with peanut and cholera toxin.

… mg of homogenized peanut (whole roasted peanuts with skins but shells removed and blended in PBS) in 0.5 mL of PBS containing 75 mg of sodium bicarbonate (Fisher Scientific, Bridgewater, NJ), 20 µg of the mucosal adjuvant cholera toxin (List Laboratories, Campbell, Calif …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell culture and reagents:

A spontaneously transformed type II mouse lung alveolar epithelial cell line [19] (C10) was cultured as described previously [20]. A C10 cell line stably expressing NF-B luciferase was generated as previously described [21]. Briefly, C10 cells were transfected with the plasmid, 6 B-tk-luc, in the presence of the plasmid PSV-2Neo (Promega), conferring a resistance to neomycin. Following incubation with the antibiotic, geneticin, antibiotic-resistant colonies were propagated. C10 cells were starved in medium containing 0.5% FBS for 16 h prior to exposure of 1 g/mL LPS (List Biological Laboratories, Inc).

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… To examine the ability of spleen cells to produce cytokines, spleen cells were cultured with or without LPS (List Biological Laboratories, Inc, Campbell, CA, USA) (5 g/mL) or …

• Product #201 – LPS from Escherichia coli O111:B4

Human Monocyte-Derived Macrophages (MDM) and MSC Direct Coculture:

Before each experiment, MSC were trypsinized, counted, washed with sterile 1X DPBS, resuspended in RPMI-1640 medium 1% FCS and added to the culture of primary human macrophages at a 1:20 MSC/MDM ratio. Cells were stimulated with LPS (Escherichia coli O111:B4, List Biological Laboratories (Campbell, California, www.listlabstage.wpengine.com) 10 ng/ml).

• Product #201 – LPS from Escherichia coli O111:B4

Reagents:

… and fimbriae 2/3 (List Biological Labs), …

• Product #186 – Fimbriae 2/3 from B. pertussis

Reagents:

…Pertussis toxin (PT) was purchased from List Biological (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)