Citations

Induction of oral tolerance and EAE:

Pertussis toxin (200 ng/mouse/injection; List Biological Laboratories) was administered i.p. at the time of immunization and 48 h later. Animals were scored for EAE as follows: 0, no disease; 1, tail paralysis; 2, hind limb weakness; 3, hind limb paralysis; 4, hind limb plus forelimb paralysis; 5, moribund.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Seven-week-old female BALB/c mice (five per group) (Japan SLC, Shizuoka, Japan) were each twice administered 30 µg of Pentamer-2, pCTB, or choleragenoid (List Biological Laboratories, Inc., Campbell, CA, USA) without adjuvant, in weeks 0 and 2, via the intranasal…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Experimental animals and surgical procedures:

…Three days before perfusion, the animals were re-anesthetized and the left sciatic nerve was exposed. Animals were injected with 3µl cholera toxin subunit B (CTB; 10mg/ml) (103B, List Laboratories Inc., Campbell, CA) in the sciatic nerve to transganglionically label ascending dorsal column axons in the spinal cord. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Toxin challenge:

We performed an in vivo challenge experiment with CT, as described previously (Tokuhara et al. 2010). Briefly, vaccinated mice (10 per group) were orally challenged with 20 µg of CT (List Biological Laboratories, Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Mouse model of FA to BLG:

…Sensitization was performed by intragastric administration on days 16, 23, 30, 37, and 44 with whey protein (WP; 15 mg/mouse; Lacprodan 80; Arla, Lyon, France) and cholera toxin (CT; 10 µg/mouse; List Biological, Campbell, CA) suspended in 100 µl of PBS. Nonsensitized mice received CT alone in 100 µl of PBS. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Anterograde Labeling of Vagal Afferents:

Each rat was given atropine (0.1 mg/ml, s.c.) 15 minutes prior to surgery to prevent airway secretions during surgery, … and CTb (1 to 2 l of 1-2% solution in deionized water, List Biological Laboratories) were pressure injected slowly into the nerve with a single-barrel glass micropipette, …

Perfusion and Immunocytochemistry:

… To examine channel content within CTb-labeled vagal afferents, alternating sections were incubated in the same cocktail with the exception of a polyclonal goat antibody directed against cholera toxin subunit B (1:25000; List Biological Laboratories, Cat: 703) instead of the IB4 antibody. …

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Histological assessment of contusion injury and phrenic motoneuron number:

…Spinal cord sections were systematically and randomly immunostained (every fourth section). Goat immunoglobulin G (IgG) anti-CTB (List Biological Laboratories Inc., Campbell, CA) and…were used as primary and secondary antibodies, respectively. The specificity of these antibodies has been validated previously.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Tolerance conditions:

To determine the effect of MPLA and LPS on inducing tolerance in HUVECs, cells were exposed combinations of…100 ng/ml of Ultra-Pure LPS (E. coli 0111:B4, List Biological Laboratories, Inc. Campbell, CA), or control (2% FBS media) for 16 hours in media containing 2% FBS (1^st treatment)…Afterwards, cells were challenged with of LPS (100 ng/ml) in 2% FBS media (2^nd treatment). LPS derived from E. coli was chosen based on previous experiments examining the inhibitory kinetics of MPLA [17], in addition to the more common use of E. coli LPS in models of endotoxic shock…

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Reagents…for stimulation of human monocytes, LPS (O111:B4) was from List Biologicals.

• Product #201 – LPS from Escherichia coli O111:B4

Ca²⁺ current recording:

In some experiments, CSMG neurons were pretreated overnight with either …or 0.5 µg/µl PTX (List Biological Laboratories, Campbell, CA) in the culture medium. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)