Bacterial Toxin Research Citations

Animals:

Female Foxd1^+/Cre (20) mice were bred with Gt(ROSA)26Sor^{tm1(HBEGF)Awai} mice to generate mice expressing the simian DT receptor (DTR) in FoxD1⁺ progenitor cells and their descendants (Foxd1Cre::RsDTR) in development and littermate controls (RsDTR). In some experiments, female Foxd1^+/Cre mice were crossed with Rosa26^{+/(STOP)-tdTomato} [B6;129S6-Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}] mice. To ablate pericytes, 25 ng/g DT (List Biological Laboratories) was injected intraperitoneally into Foxd1Cre::RsDTR mice or littermate controls. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Tolerance Induction and Food Allergy Model

Tolerance was induced by intragastric administration of 1 mg peanut extract (PE) daily for 5 consecutive days. The murine model of food allergy has been previously described (Li et al., 2000); briefly, mice were sensitized by administration of 1 mg PE with 10 μg of cholera toxin (List Biologicals) in 200 μl of PBS intragastrically on days 0 and 7, followed by a booster challenge with 10 mg PE on day 21. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction:

DAPK[CAM]-transgenic, DAPK-knockout, and their WT littermate control mice were immunized subcutaneously with 200µg of MOG peptide 3555 … followed by intraperitoneal injection of 200ng pertussis toxin (List Labs, Campbell, CA, USA) at day 0 and day 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For treatments, the LSM was amended with 2 g/ml protective antigen (PA) or 2 g/ml lethal toxin (LT, 2 g/ml PA plus 2 g/ml LF, List Biological Laboratories). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… EAE was induced in 8- to 12-week-old female WT or S1PR1(S5A) mice immunized with CFA, MOG 3555 , and Bordetella pertussis toxin (List Biological Laboratories) at 400 ng per mouse on days 0 and 2 (43). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cholera toxin was purchased from List Biological Laboratories (Campbell, CA).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Injections were carried out on WT and vGluT1 mutant mice at p8 and p12 (n R 3 for each genotype and developmental time point), and spi- nal cords were harvested at p12 and p21, respectively. CTb staining was enhanced with anti-CTb antibody, 1:1,000 (List Biological Laboratories Inc.).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

…For the 2-day experiments assessing tolerization against cell activation by E. coli LPS (TLR4 ligand ultrapure grade, from E. coli O111:B4; List Biological Laboratories, Inc., Campbell, CA), …were incubated in 96-well plates. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

…,according to themanufacturer’s instructions as a positive control, the endothelial cells were incubated with LPS (1 ng/mL) from Escherichia coli 0111.B4 (List Biological Laboratories, Campbell, CA). …

• Product #201 – LPS from Escherichia coli O111:B4

EAE:

… Mycobacterium tuberculosis H37Ra (Difco Laboratories). At the time of and 2 d after immunization, mice were injected ip with 300 ng Bordetella pertussis toxin (List Biological Laboratories) in PBS. Mice were examined daily …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)