Bacterial Toxin Research Citations

Depletion of regulatory T cells in DEREG mice:

For conditional ablation of Tregs, DEREG mice were injected intraperitoneally [ip] twice with 1 g of diphtheria toxin [DT] [List Biological Laboratories, Campbell, CA, USA] 1 day before and on the first day of DSS treatment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

RNA interference in RAW264.7 cells:

… Transfections were carried out in 96-well format using 200,000 cells/well and 2 m siRNA. Thirty-six hours after siRNA transfection, LTA was added to a final concentration of 2.5 g/ml LTA. Supernatant was collected 5 hr post-LTA treatment, and cytokine production was assayed using DuoSet ELISA kits (R&D Systems). In experiments monitoring the response to lipopolysaccharide (LPS) instead of LTA, LPS was added at a final concentration of 20 ng/ml (LPS from List Biological Laboratories, Campbell, CA).

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Cell culture:

… On the next day, the adherent macrophages were incubated in serum-free medium RPMI1640 at 37C for 2h and then stimulated with LPS (List Biological Laboratories, Escherichia coli 055:B5), …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

…cholera toxin (List Biological Laboratories, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Electrolyte Solutions and Analytes:

… Anthrax toxins PA63 and LF were obtained from List Biological Labs, Inc. (Campbell, CA, USA). …

Discussion:

We also used EIS to estimate the biochip receiver operating characteristic for the detection of B. anthracis LF. For example, we determined the sensitivity of the PA63 chip to LF (<50 pM), and the response time of the system (minutes). … Antibody 2C11 does not appear to block LF binding to the membrane, which is consistent within vivo measurements of LF activity [76].

… In particular, we demonstrate that AB toxins such as the LF:PA63 from B. anthracis [40] provide a highly specific, built-in sensing scheme.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

… Emulsions were prepared using an Omni-Mixer (Omni International, Warrenton, VA). Mice also received 300 ng pertussis toxin (List Biological Laboratories, Campbell, CA) intravenously at the time of immunization and 72 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… All mice received 200 ng of pertussis toxin (List Biological Laboratories, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antibodies and dyes:

…PTX was obtained from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…8-12 week-old mice were immunized subcutaneously at the base of the tail with 100 µg of indicated peptides dissolved in 100 µL of PBS, emulsified in 100 µL CFA (Difco) supplemented with 500 µg M. tuberculosis H37RA (Difco). For active EAE, pertussis toxin (List Biological Laboratories, Campbell, CA) was injected i.v. at days 0 and 2 (400 and 200 ng respectively). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… At 0 h and 48 h after immunization, mice were intraperitoneally injected with 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)