Bacterial Toxin Research Citations

C3H/HeJ mice were rendered peanut allergic by means of oral sensitization with peanut and cholera toxin.

… mg of homogenized peanut (whole roasted peanuts with skins but shells removed and blended in PBS) in 0.5 mL of PBS containing 75 mg of sodium bicarbonate (Fisher Scientific, Bridgewater, NJ), 20 µg of the mucosal adjuvant cholera toxin (List Laboratories, Campbell, Calif …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell culture and reagents:

A spontaneously transformed type II mouse lung alveolar epithelial cell line [19] (C10) was cultured as described previously [20]. A C10 cell line stably expressing NF-B luciferase was generated as previously described [21]. Briefly, C10 cells were transfected with the plasmid, 6 B-tk-luc, in the presence of the plasmid PSV-2Neo (Promega), conferring a resistance to neomycin. Following incubation with the antibiotic, geneticin, antibiotic-resistant colonies were propagated. C10 cells were starved in medium containing 0.5% FBS for 16 h prior to exposure of 1 g/mL LPS (List Biological Laboratories, Inc).

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… To examine the ability of spleen cells to produce cytokines, spleen cells were cultured with or without LPS (List Biological Laboratories, Inc, Campbell, CA, USA) (5 g/mL) or …

• Product #201 – LPS from Escherichia coli O111:B4

Human Monocyte-Derived Macrophages (MDM) and MSC Direct Coculture:

Before each experiment, MSC were trypsinized, counted, washed with sterile 1X DPBS, resuspended in RPMI-1640 medium 1% FCS and added to the culture of primary human macrophages at a 1:20 MSC/MDM ratio. Cells were stimulated with LPS (Escherichia coli O111:B4, List Biological Laboratories (Campbell, California, www.listlabstage.wpengine.com) 10 ng/ml).

• Product #201 – LPS from Escherichia coli O111:B4

Reagents:

… and fimbriae 2/3 (List Biological Labs), …

• Product #186 – Fimbriae 2/3 from B. pertussis

Reagents:

…Pertussis toxin (PT) was purchased from List Biological (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Study Design and Animal Model:

In 34 mice, experimental autoimmune myocarditis (EAM) was induced by intraperitoneal injection of 400-ng pertussis toxin (List Biological Laboratories) followed 1 hour later by subcutaneous injection of…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

8-10 week old male B10.PL mice were immunized subcutaneously at four sites in the flanks…In addition, 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) was injected i.p. on days 0 (0 h) and 2 (45 h).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… In addition, mice were weekly challenged with 10 ng SEB (List Biological Laboratories Inc., Campbell, CA) from day 49 through day 102 (8 weeks, short-term group) or day 186 (20 weeks, long-term group) after OVA instillation. …

Surgical Procedures

C5 Unilateral Spinal Cord Contusion

… Three days prior to being euthanized rats in Experiment 2 were anesthetized with XAK, and an axial incision to the ipsilesional (right) forelimb exposed the median and ulnar nerves,26 and the perineurium was removed. A 5-µL Hamilton syringe fitted with a custom 30 gauge needle (#7803-07, Hamilton Company, Reno, NV) was inserted into each nerve, and 2.0 µL of cholera toxin β subunit (CTB; 10 µg/µL; Product #104, List Biological Laboratories, Inc, Campbell, CA) was injected to label myelinated sensory afferents. Rats received 5 cc of LRS and cefazolin subcutaneously following surgery.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt