Bacterial Toxin Research Citations

2.1. Animals—Experimental Autoimmune Optic Neuritis Model

Female C57BL/6 mice (7–8 weeks of age) were purchased from Samtaco (Osan, Republic of Korea). … Pertussis toxin (List Biological Laboratories, Campbell, CA, USA) dissolved in PBS (200 ng/mouse) was injected intraperitoneally on the day of immunization and again at 48 h post-immunization [20]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Histology and neuronal tracers

Some degus (n=2) were used only for neuroanatomical data collection. The labeling of retinal projections to the SC was performed under gaseous anesthesia using isoflurane (4% in medical oxygen at a flow rate of 100 cc/kg/min) complemented with diazepam (5 mg/kg) administrated IP. A glass pipette (tip diameter 15–20 μm) containing 1% cholera toxin subunit B (CTB, List Biological Laboratories, Campbell, CA) in 0.1 M phosphate buffer was injected intraocularly. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of experimental autoimmune encephalomyelitis (EAE) and clinical scoring

To induce active EAE, mice were immunized at day 0 with 50μg of MOG35-55 peptide (Covalab) emulsified in complete Freund’s adjuvant (CFA) composed of Mycobacterium Tuberculosis 1mg/ml (Becton-Dikinson) in incomplete Freund’s adjuvant (Sigma-Aldrich-F5506). Pertussis toxin 200ng/ml (List Labs) was injected intravenously (i.v.) at days 0 and 2 post-immunization. The incidence and the severity of the disease were monitored daily and were evaluated as previously described (56).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… A mixture of rabies virus (CVS strain, 5.96?×?1010 PFU/ml) and the conventional tracer Cholera toxin B fragment (CTB low salt, end concentration 0.03%) (List Biological Labs, Campbell, CA) was injected in a single dose (2 ?l delivered over 30?min) into MSTl (2 monkeys), rostral MIP (2 monkeys) or LIPv (1 monkey) at midpoint of the rostrocaudal length of the IPS (Figure 1) using a Hamilton syringe driven by a micromanipulator. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… All mice were intraperitoneally injected with 200 ng of pertussis toxin (PTX; List Biologicals, Campbell, USA) on the day of the last immunization and 2 days later. The brains were harvested after behavioral tests and stored at -80 °C for the following studies. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

STAR+METHODS
KEY RESOURCES TABLE

Chemicals, peptides, and recombinant proteins

Pertussis Toxin (PT) List Biologicals 180
Tetanus Toxoid (TT) List Biologicals 191A
Pertactin (PRN) List Biologicals 187
Fimbriae 2/3 (Fim2/3) List Biologicals 186
Diphtheria Toxoid (DT) List Biologicals 151

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #191 – Tetanus Toxoid from Clostridium tetani
• Product #187 – Pertactin from B. pertussis (69 kDa Protein)
• Product #186 – Fimbriae 2/3 from B. pertussis
• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae

2.2. Immunization and collection of specimens

Mice were anesthetized with isoflurane before receiving the vaccine preparation for intranasal immunization. A 20-µl phosphate-buffered saline (PBS) solution containing the Mtb antigen ESAT-6 (5 µg; MyBioSource; MBS204541) in the presence or absence of S100A4 (10 µg; Gentaur Molecular Products; 01-2081A4M; with His-tag) was administered dropwise to the external nares of the mice (10 µl per nostril). Some mice were immunized with ESAT-6 (5 µg) admixed to cholera toxin (CT; 1 μg; List Biological Labs; 100B) as a control adjuvant. Ten days after the last intranasal immunization, various tissues and samples were collected and analyzed, including blood, bronchoalveolar lavage fluid (BALF), nasal lavage, lungs, and spleen.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

STAR+METHODS
KEY RESOURCES TABLE

CTB List Biological Laboratories Cat: 103B
CT List Biological Laboratories Cat: 100B

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of EAU model

On the initial day of the experiment, the mice were administered a subcutaneous injection containing a 1:1 combination of 200 μg of human interphotoreceptor retinoid-binding protein 1–20 (IRBP1–20, GPTHLFQPSLVLDMAKVLLD; GiL Biochem, Shanghai, China) and complete Freund’s adjuvant (Difco, Detroit, MI, USA). The complete Freund’s adjuvant consisted of 2.5 mg/mL of Mycobacterium tuberculosis (BD Difco, San Jose, CA, USA). On the same day of immunization and two days thereafter, an intraperitoneal injection of 0.25 μg pertussis toxin (PTX) diluted in phosphate-buffered saline (PBS) was administered. The PTX used in this study was obtained from List Biological Laboratories located in Campbell, California, USA.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of MOG 35-55 peptide-induced EAE

On Day 0, ~12-week-old female mice were immunized subcutaneously with 200 µg of MOG 35-55 peptide (MEVGWYRSPFSRVVHLYRNGK)118 and 400 µg of Mycobacterium tuberculosis H37Ra (MTb, Difco, Detroit, MI). Incomplete Freund’s Adjuvant (IFA; Gibco, Grand Island, NY) and dried, heat-killed MTb were emulsified with an equal volume of MOG 35-55 dissolved in PBS using a VirTishear Cyclone homogenizer with a 10 mm micro shaft impeller (VirTis, Gardiner, NY). The mice were immunized with 150 µl of the MOG-containing emulsion divided among three subcutaneous sites on the flank (between the shoulder blades and over each hip). Additionally, on Days 0 and 2, the mice were injected intraperitoneally with 200 ng pertussis toxin (PTx) (List Biological Laboratories, Campbell, CA) in 200 µl PBS119.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)