Bacterial Toxin Research Citations

2. Materials and Methods

… Semi-rough (Rc) LPS—E. coli J5 and Rough (Re) LPS—E. coli K12 D32m4—was purchased from List Biological Laboratories, …

2.3. Bacterial Infection
LPS was resuspended in LAL reagent water (<0.005 EU/mL endotoxin levels). Adding 100 ng/mL of either lipopolysaccharide serotype to a flask containing RAW264.7 cells stimulated the desired inflammatory response for 6 h, after which the culture medium was removed, and the cells were collected for analysis. …

• Product #301 – LPS from Escherichia coli J5 (Rc)
• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)

… Mice were then intravenously injected with 200 ng bordetella pertussis toxin (180243A2, List Labs) immediately after immunization. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… To deplete Foxp3+ Treg in Foxp3-DTR mice, DT (List Biological Laboratories, California, USA) 500 ng per mouse was injected intraperitoneally (i.p.) on days four and six after the start of MC903 treatment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents

… pertussis toxin (179B, List Biological Laboratories); …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (200 ng, List Biologics, Campbell, CA) was administered intraperitoneally on the day of MOG immunization and repeated 1 day after. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… List Biological Laboratories (1 mg/vial) 10069A1 – 12 EU/mgc 0.0010 EU/mLd 10068A1 – 45 EU/mgc 0.0038 EU/mLd 10067A1 – 5.0 EU/mgc 0.00042 EU/mLd a The Same lot … List Biological Laboratories is one of the candidates since toxin manufactured by List Biological Laboratories and toxin manufactured by List Biological Laboratories…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAU model development and disease grading

A 1:1 mixture containing 2 mg/ml interphotoreceptor retinoid-binding protein 1–20 (IRBP1 − 20, GiL Biochem, Shanghai, China) and complete Freund’s adjuvant (CFA, BD Difco, San Jose, CA, USA) with 2.5 mg/ml of Mycobacterium tuberculosis (BD Difco, San Jose, CA, USA) was subcutaneously injected into mice (0.2 ml per mouse) on day 0. After immunization with IRBP1 − 20, mice were intraperitoneally injected with 0.2 µg pertussis toxin (PTX, List Biological Laboratories, Campbell, CA, USA) dissolved in phosphate-buffered saline (0.1 ml per mouse) immediately. On day 2, PTX was again intraperitoneally injected into mice at the same dose [6]. On day 14, we conducted funduscopic examinations on the mice. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tamoxifen and diphtheria toxin treatment

Tamoxifen (MedChemExpress) was dissolved in corn oil (20 mg ml−1) and heated at 37°C for 3 hours. To activate Cx3cr1CreERT2-mediated recombinase, each mouse was injected with Tamoxifen (100 mg/kg body weight) by oral gavage every three days, starting from Day 12 post-intraportal injection, as indicated in Fig. 2e. For proliferation tracing experiment, DreERT2-mediated recombinase was introduced by a single dose of tamoxifen gavage (100 mg/kg body weight) on Day 12 post-intraportal injection, as indicated in Fig. 3f. For KC specific depletion, DreERT2-mediated recombinase was introduced by 8 doses of tamoxifen gavage (2 mg per dose), as indicated in Fig. 5d. A single dose of 200 ng DT (List Laboratories) was intraperitoneally introduced one week after the last Tamoxifen injection. The cancer cells were inoculated into liver through portal vein one day after DT injection.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Diphtheria Toxin (149A) was purchased from List Laboratories (CA, U.S.). …

… Treg depleted Rag 1-/- mouse model: 5 × 105 CD4+CD45RBhi T cells purified from the spleen and MLN of B6.129(Cg)-Foxp3/J donor mice were intraperitoneally injected into Rag 1-/- mice to construct a colitis model. During the experiment, 10 µg/kg DT was intraperitoneally injected into Rag 1-/- mice every 7 d from week 1, onward. B6.129(Cg)-Foxp3/J mice, which are Foxp3DTR knock-in mice. These mice contained a human DTR and GFP inserted downstream of the internal stop codon of the X-linked Foxp3 gene. Foxp3+ Tregs in these mice highly express DTR-GFP. Administration of DT to these mice resulted in the depletion of Treg cells in lymphoid tissues within 2 d post-injection. …

• Product #149A – Diphtheria Toxin CRM197, Mutant
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Tracer Injections

… Five monkeys (M127, M128, M131, M132, M140) were injected with 70-120nl retrograde tracer Cholera Toxin subunit β (CTb, 1% low salt solution, List Biological Labs, USA), one female monkey (M129) was injected with 80-100nl retrograde tracer Fluorogold (FG, Fluorochrome LCC). After 87-278 hours, animals were perfused and the tissue processed.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt