Citations

Culture supernatant extract anthrax vaccine and its liquid and dry powder formulations preparation and characterization

… Total protein concentrations of the CSE vaccine were measured by bicinchoninic acid assay (BCA, Gene-Star, Beijing, China). An enzyme-linked immunosorbent assay (ELISA, Dakewe, Beijing, China) was used to quantify the PA protein in the CSE vaccine according to a previously method [Citation20]. Serially diluted commercial recombinant PA (rPA, 171E, List Labs, Campbell, CA, USA) was the reference standard. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Pertussis toxin (200 ng/mouse, i.p.; List Biological Laboratories) was injected on the days 0 and 1 relative to immunization. Clinical scores were monitored daily in a blinded manner and recorded as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Human Endotoxemia (IV LPS Administration)

… Following that, it was continuously given at a rate of 150mL/h for the remaining duration of the experiment. Promptly after prehydration, a bodyweight-adjusted bolus dose of 1ng/kg LPS (Escherichia coli-derived, Type O0113, lot no.94332B1; List Biological Laboratories, Campbell, CA, USA) was administered. Blood samples were serially obtained to construct time-concentration curves of circuiting cytokines (Jansen et al., 2022). Temperature and cardiovascular monitoring (blood pressure and heart rate) and sickness symptoms (headache, muscle pain, back pain, nausea, shivers and vomiting were recorded throughout the whole experiment with intervals of 30 minutes. …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

… The emulsion (200 µL/mouse) was injected subcutaneously at three points on the back of each mouse, and a pertussis toxin (250 ng/mouse) (List Biological Laboratories, 181, CA, USA) solution was intraperitoneally injected at 0 h and 48 h. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… For treatment with pertussis toxin (PTX), the cells were cultured with 100 ng/ml PTX (List Biological Laboratories, Campbell, CA; …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials and Methods

… Rc LPS – E. coli J5, List Biological Laboratories, 301; Re LPS – E. coli K12 D32m4, List Biological Laboratories, 302]. …

• Product #301 – LPS from Escherichia coli J5 (Rc)
• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)

… EAE model, as previously described (Yarim et al. 2022). After (MOG)(35-55) injection, intraperitoneal injections of 200 ng pertussis toxin (List Biological Laboratories, USA) in the first hour and 400 ng pertussis toxin in 200 µl PBS were administered at the hour 48. The experimental design of the groups and EAE induction procedure are shown in Fig. 2 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tract tracing and immunohistochemistry

For tracer injections, bats were anesthetized by isoflurane inhalation in a custom stereotaxic apparatus. A 1mm x 1mm craniotomy was made over the primary motor cortex. A tracer deposit via iontophoretic injection (5μA, 10 min) at varying depths was made targeting the deep layers of the primary motor cortex using 10% biotinylated dextran amine (BDA; 10,000 MW) (Life Technologies) (n=4, males) or 1% cholera toxin subunit B (CTB, List Biological Laboratories) in nucleus ambiguus/brainstem (n=2, males). …

The primary antibody solution contained of goat anti-ChAT (Millipore AB144P, 1:200) and anti-CTB (List Biological Laboratories, 1:10,000) antibodies, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Staphylococcal Enterotoxin Type B (SEB) (List Biological Laboratories Cat#122) was a positive assay control, …

EAE induction

Female mice (7–10 weeks old) were prepared for acclimatisation at least 1 week before the experiment and immunised with an emulsion containing 150 μg of I-Ab MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK; MBL Life science, Japan, cat#: TS-704-P) and 500 μg of Mycobacterium tuberculosis extract H37RA in complete Freund’s adjuvant (Chondrex, WA, USA, cat#: 7023) by subcutaneous injection on the both sides of the back. 400 µg of Pertussis toxin (List Biological Laboratories, CA, USA, cat#: 180) diluted in D-PBS (nakarai tesque, cat#: 14249-24) was intraperitoneally administrated at 0- and 48-h postimmunization. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer