Citations

… Pertussis toxin, lyophilized in buffer (List Biological Laboratories Inc.). Resuspend in 1 ml of sterile dH 2O for a concentration of 50 µg/ml and store at 4°C ( see Note 7). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin from Bordetella pertussis (List Biological Labs). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Histochemistry and immunohistochemistry:

Rats were transcardially perfused with room-temperature 0.1 M sodium phosphate buffer, pH 7.3, followed by ice-cold 4% paraformaldehyde in phosphate buffer. Brains were postfixed… Conventional or immunofluorescence protocols were used to reveal the localization of anterograde/retrograde tracers or proteins of interest, following previously described methods (Deutch et al., 1996). For animals receiving nonconjugated CTb injections, a goat anti-CTb antibody (List Biological, Campbell, CA; RRID:AB_10013220) was used.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… In addition, animals received 300 ng of Pertussis toxin by intraperitoneal (ip) route (List Biological Laboratories, Inc., Campbell, CA, USA) on days 0 and 2 post-immunization. Non-immunized (nave) animals were used as control group. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antibodies, reagents and medium:

… CT was from List Biological Labs (Campbell, CA, USA). …

In vivo sensitization, and BLG specific challenge

Mice were sensitized 5 times 1 week apart by oral administration of 10 µg of CT in association with 10 mg of antigen in a solution containing 0.2 M of NaHCO3, pH 9. An oral antigen challenge was performed 1 week after the last sensitization by gavaging 100 mg BLG….

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of casein-mediated food allergy in mice

Female mice were sensitized with 1 mg of casein (Sigma-Aldrich, St. Louis, MO) plus 10 μg of cholera toxin (CT, List Biological Laboratories, Inc., Campbell, CA) by oral administration (p.o.) in 200 μl PBS on days 0 and 7. After this, mice were orally boosted four times for another week, with 5 mg of casein plus 10 μg of CT. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunization protocol for MOG-induced EAE:

…All mice received an intraperitoneal (ip) injection with 150 µl of pertussis toxin (300 ng) (List Biological Laboratories, Campbell, CA) immediately after immunization and again 2 days later. Mice

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Anatomical tracers studies

Surgeries.

… For tracer injections, glass capillaries of 10 –25 m external tip diameter were backfilled with either a 1% solution of cholera toxin subunit B
(CTb; #104, List Biological Laboratories), dissolved in 0.05 M sodium phosphate buffer, a 2% solution of FluoroGold (FG; Fluorochrome),
dissolved in 0.9% saline, or a 5% solution of biotinylated dextran amine (BDA), 10,000 MW (#D1956, Invitrogen), dissolved in 0.1 M sodium
phosphate buffer. Discrete CTb and FG tracer deposits were made by iontophoresis (7 s on/off for 3–12 min; 1–5 A) in the PaMP region (0.7
mm posterior from bregma, 0.1 mm lateral from the center of the longitudinal sinus, and 7.2 mm ventral from the dura) or the RPa region (2.0
mm posterior from the interaural line, centered at the interaural line, and 8.8 mm ventral from the dura) as described in Table 1. In additional
animals, a deposit of the anterograde tracer BDA was made in the rPH (2.1 mm posterior from bregma, 0.2 mm lateral from the center of the
longitudinal sinus, and 7.4 mm ventral from the dura) to verify the results obtained with the retrograde tracers CTb and FG. Animals were
allowed to recover for 2–3 weeks before euthanasia. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Dosage of anti-IL-6 and anti-Tetanus toxoid antibodies by ELISA:

Blood samples were taken from the monkeys in pre-dose (day -8), before the first immunization and then every two weeks until the end of the study (total of 9 samples). The IgG response against human IL-6 and Tetanus toxoid (TTx) was measured by ELISA as previously described¹⁷. Briefly, 50ng of human IL-6 (R&D Systems, Lille, France) or 50ng of TTx (List Biological Laboratories, Campbell, USA) were adsorbed on microtitration plates…

• Product #191A – Tetanus Toxoid from Clostridium tetani

EAE:

All mice also received 100 ng pertussis toxin (List Biological Laboratories) intraperitoneally on days 0 and 2. Mice were assigned clinical scores for EAE according to the following scale: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)