Citations

 The ALF-type particles utilized in the original studies with A1-15 peptide contained native MPLA derived from gram-negative bacterial lipopolysaccharide as supplied by List Biological Laboratories (Campbell, CA USA), …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Disease induction and EAE analysis:

EAE was induced in female C57BL/6 mice (Jackson Laboratories, Bar Habour, Maine), aged 8–9 weeks, by injecting subcutaneously (s.c.) 50μg of myelin oligodendrocyte glycoprotein peptide (MOG)35–55 in Complete Freund’s Adjuvant (CFA) (Fisher, Michigan USA) on day 0 [24–25]. Intraperitoneal (i.p.) pertussis toxin (0.1μg/200μl, List Biological labs, Hornby, ON) was administered on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In vitro culture of T cells:

Antigen-presenting spleen cells or DCs were pulsed with 1gml¹ staphylococcus enterotoxin B (SEB; List Biological Laboratories)…

Animals, antigens and adjuvants:

Six-week-old female and male C3H/HeJ mice were purchased from the Jackson Laboratory (Bar Harbor, ME). BALB/c female mice (58 weeks old) were obtained from Taconic Farms (Hudson, NY). … Cholera toxin (CT) was purchased from List Biological Laboratories Inc (Campbell, CA).

Sensitization, allergen challenge and assessment of anaphylaxis:

Five-week old C3H/HeJ offspring were sensitized with CPE+CT by gavage (5 mg CPE + 20 µg CT/mouse) at weekly intervals for 6 weeks followed by 2 boosters at 2-week intervals (50 mg CPE + 20 µg CT/mouse), as previously published…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cholera toxin (azide-free) from Vibrio cholerae used for cAMP experiments was purchased from List Biological Laboratories (Campbell, CA) (catalog no. 100B).

Measurement of cAMP:

…Cells were incubated with forskolin (10 M), VIP (1 M), or cholera holotoxin (0, 1, or 100 nM) in culture media with 5% CO₂ at 37°C for 1hr, followed 2 washes with cold PBS, then lysed and flash frozen in liquid N₂…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunohistochemical Methods:

Immunocytochemical detection was performed by standard light, fluorescent microscopy…Rats were perfused transcardially (4% paraformaldehyde solution). Sections were incubated (overnight/4C)…goat anti CTb primary antibody (1/10,000; List Biological Laboratories).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE induction and disease scores:

EAE was induced in C57BL/6 (The Jackson Laboratory, Bar Harbor, ME) female mice (8-10 weeks old). …Pertussis toxin (List Biological Labs Inc., Campbell, CA) was administered by intraperitoneal (ip) injection at day 0 (75 ng per mouse) and day 2 (200 ng per mouse) after immunization. Mice were scored daily for EAE …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis:

… For active EAE, Sort1 / and WT littermate mice were immunized sc with 250 µg MOG 3555 or whole-protein MOG…and received 200 ng pertussis toxin (List Biological Laboratories) ip at the time of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tracers and coordinates:

Retrograde tracing was accomplished using 3-10nl injections of Cholera toxin subunit B (CTb) (10 µg/µl saline, List Biological, Campbell, CA), whereas anterograde tracing was done using either 3-15 nl injections of biotinylated dextran amine (BDA) …

<• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Recovery Surgical Procedures: Retrograde Tracer Injections and Catheters (Studies 2 and 3):

…In rats to be used in study 2, one of the following retrograde tracers was microinjected into the left dorsal lateral column containing preganglionic sympathetic fibers: Fluoro-Gold (FG; 2% in deionized water; Fluorochrome, Denver, CO; n = 2), cholera toxin subunit (CTB; 1% in deionized water; List Biological Laboratories, Campbell, CA; n = 7), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt