Bacterial Toxin Research Citations

Reagents:

Purified lipopolysaccharides (LPS) from Escherichia coli were obtained from List Biological Laboratory (Campbell, CA) and dissolved in endotoxin-free water. …

Immunoblot assay with hepatocytes and liver tissue:

For immunoblotting of caspase-1 and IL-1, hepatocytes were plated in 6-well plates (810⁵ cells/ml) and primed with LPS (500ng/ml) for 4hr. 

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Surgical procedures performed in mice: 

Tract tracing was performed by injection of Cholera toxin B (CTB) (List Biological Laboratory, Campbell, CA) 1 µl of 1% wt/vol in sterile water injected into both sciatic nerves three days before perfusion to visualize ascending sensory tract (AST) axons.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of EAE:

Mice (8-12 weeks old) were immunized subcutaneously in the hind limbs on day… Two hundred nanograms of pertussis toxin (PTX) (List Biological Laboratories, Campbell, CA) was injected intraperitoneally on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To determine whether TRPV3 channels are associated with the DA neurons in pVTA that project to the Acb, the retrograde neuronal tracer, cholera toxin- subunit (CtB; List Biological Laboratories), was iontophoresced into the Acb (n = 6) as described previously…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Immunization protocol and treatment of mice with antibiotics:

We anesthetized 6~8-week old age- and sex-matched mice with isoflurane and intranasally immunized the mice with 30 µl of PBS (Gibco) containing 30 µg of HSA and 100 ng of CT using a pipette and tip. For secondary immunization, mice were challenged with HSA (30 µg) and CT (100 ng) four weeks after the primary immunization.

For systemic immunization, mice were intraperitoneally injected with 100 µl of PBS containing 100 µg of HSA and various concentrations of CT.

Reagents: Cholera toxin (CT; List Biological Laboratories)

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunofluorescent study:

Each section was pretreated in the same way as described above for immunohistochemistry. For the detection of GM1 ganglioside, biotinylated cholera toxin B subunit (1:500; List Biological Laboratories, Inc.) and …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

… Alternatively, macrophages were stimulated using E. coli lipopolysaccharide (100 ng ml 1 ; Sigma-Aldrich, St Louis, MO, USA), Anthrax protective antigen and Anthrax lethal factor (List Biological Laboratories, Campbell, CA, USA), …

• Product #171 – Anthrax Protective Antigen (PA),Recombinant from B. anthracis
• Author did not specify which List Labs Lethal Factor was utilized.  List Labs offers Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Materials:

We purchased BoNT/A of 150 kDa molecular weight from List Biological (Campbell, CA, USA) in a formulation containing 80% saturated ammonium sulphate. …

Materials and Methods:

BoNT/A was radiolabelled by pretreatment with 2iminothiolane and incubation with 99mTc-gluconate. The labelled toxin 99mTc-BoNT/A was puried using size exclusion HPLC. Twenty-four female Wistar rats were evenly injected in the bladder wall with either 99mTc-/ (n = 12) or free 99mTc (n = 12). Four rats from each group were killed at 1, 3 and 6 h after injection, respectively. The bladder, L6-S1 spinal cord segment and L6-S1 dorsal root ganglia (DRG) were harvested and their radioactivity counted in a gamma scintillation detector. Results were calculated as % injected dose (I.D.) per gram of tissue. The paired t-test was used for comparison of means of 99mTc-/ radioactivity vs free 99mTc in the tissues of interest.

…All 45 mice were divided in five groups, with nine mice in each group. The first is the control group, which was exposed to 10 µg of cholera toxin (List Biological Laboratories, Inc., Campbell, CA, USA) only. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction and analysis:

EAE was induced in 8 to 12 week-old mice by immunization with 100 µg MOG p35-55 or rhMOG in complete Freund’s adjuvant containing 200 µg Mycobacterium tuberculosis H37Ra (Difco Laboratories, Detroit, MI) on day 0. Mice received 200 ng Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA) IP on days 0 and 2. Mice were observed daily for clinical EAE.² Histology and immunohistochemistry analyses were performed as previously described.¹

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)