Bacterial Toxin Research Citations

Polyclonal CD4⁺ T cell adoptive transfer (in mice):

Foxp3-expressing T_reg cells were ablated following adoptive transfer by the i.p. injection of diphtheria toxin (DT; List Biological, Campbell, CA) at a concentration of 0.5 g/mouse on day 1 after adoptive transfer of T cells, and then 0.2 g/mouse every other until day 21.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Conjugation of Dylight 594 to CTXB:

For GM1 ganglioside detection, Dylight 594 (Thermo Cat #46412) was conjugated to the primary amines of Cholera Toxin B protein (CTXB) (List Biologicals Cat #104) following manufactures protocol. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

CT ELISA Assays:

CT production was measured using V. cholerae culture supernatants by GM1-enzyme-linked immunosorbent assays (GM1ELISA) as described previously [39]. Purified CT subunit B (List Biological Laboratories Inc., Campbell, CA, USA) was used to provide a standard curve and phosphate-buffered saline (PBS) was used as a negative control. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Stereotaxic surgery:

Ten 49118 days old male C57BL/6 J wild-type mice were anesthetized with isoflurane followed by an intraperitoneal injection of an anesthetic mixture. …In these cases, following FG injections, we injected the retrograde tracer 0.5 % Cholera toxin B subunit (CTB, List Biologicals, Campbell, CA, USA) into the HIPP of the left hemisphere at three coordinates: 2.5, 2.8, 3.2 mm posterior from bregma, 1.6, 1.6, 1.7 mm lateral from midline and 2.1, 2.1, 2.2 mm below the zero level, respectively. In the other four mice, FG was injected bilaterally into the PFC at the coordinates given above, and CTB was injected into the MS at the coordinates 1.0 mm anterior from bregma, in the midline and 4.1 mm below the zero level. We injected 1418 nl of FG and 1823 nl of CTB into every single injection spot using the Nanoject-R pump. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… weeks. Four days before sacrifice, 2 µL of 1 % cholera toxin B (CT-B; List Biologicals) was injected in the left sciatic nerve. Fifty-micrometer sagittal free floating sections were immunostained for CT-B (List Biologicals, 1:30,000). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Experimental autoimmune encephalomyelitis:

… Pertussis toxin (PTX; 200 ng; List Biological Laboratories) was administered intraperitoneally on the day of immunization and on day 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… PTX List Biological Laboratories, Inc, Campbell, USA SCH-58261 S4568-5MG, …

Induction, treatment and clinical evaluation of EAE:

6-8 weeks old female mice with C57BL/6 background purchased from The Janvier Laboratory were used for the experiments. Animals were 6 weeks old and weighed between 19 and 23 µg. Each mouse was immunized subcutaneously (s.c.) at day 0 with 200 µg MOG35-55 peptide and 800 µg MTB H37RA dissolved in 100 µl PBS and 100 µl CFA. Emulsion was sonificated with a Bandelin Sonopuls at 50 % interval and 50 % power for 3 minutes. Additionally, 400 ng of PTX was applied intraperitoneally (i.p.) at day 0 and day 2. Omnifix-F syringes were used for all injections. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and evaluation of EAE:

EAE was induced by injecting mice s.c. (in the back region) with MOG₃₅₅₅ peptide (200 µg/mouse) in CFA containing 5mg/ml heat-killed Mycobacterium tuberculosis … In the day of immunization and 48h later, the mice were also injected i.v. with pertussis toxin (200ng/mouse; List Biological Laboratories, Campbell, CA) in PBS. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cells and Reagents:

Ultrapure E. coli O111:B4 LPS was purchased form List Biological Laboratories.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Induction and assessment of active EAE:

EAE was induced in B6 mice as previously describe… In addition, mice received 200 ng pertussis toxin (pertussis toxin in Glycerol, List Biological Laboratories) i.p. on the day of immunization and 2 days thereafter. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)