Bacterial Toxin Research Citations

Binding analysis of the TTC fusion protein to B-lymphocytes by flow cytometry:

… For comparison, a FITC-labeled TTC peptide (List Biological Laboratories, Inc., CA, USA) was used. …

CT was purchased from List Biological Laboratories (Campbell, CA)…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… sc) of 0.2 ml inoculum containing 200 µg MOG peptide 35-55 (MEVGWYRSPFSRVVHLYRNGK) in IFA (DifcoLabs, Detroit, MI) with 800 g Mycobacterium tuberculosus hominis H37Ra (MT) on day 0 and 7 following [19], with pertussis toxin (PTx) (List Biologicals) 200 ng ip on …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice and EAE induction:

…In most experiments IL-12R2^/ and WT mice were immunized for EAE induction with 200 µg PLP₁₃₉₁₅₁ (GenScript) emulsified in complete Freunds adjuvant (CFA) containing 3 mg/ml Mycobacterium tuberculosis (H37Ra; Difco Laboratories). In addition, mice were given 200 ng pertussis toxin (List Biologicals Laboratories) i.p. on days 0 and 2 post-immunization (p.i.). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… emulsified with complete Freund’s adjuvant (CFA) containing 500 µg of Mycobacterium tuberculosis H37RA (BD Biosciences) on day 0 and were intraperitoneally (ip) injected with 500 µg of pertussis toxin (PTX; List Biological Laboratories, Campbell, CA) on days 0 and 2 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

…EAE was induced by s.c. immunization at four sites on the back with 100 mg rhMOG emulsied in CFA containing 5 mg/ml mycobacteria… On days 0 and 2, mice were injected i.p. with 300 ng pertussis toxin (List Biological Laboratories, Campbell, CA).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Retrograde tracing and histology:

… We anaesthetized the Pdyn-ires-Cre; Z/EG and the Pdyn-ires-Cre; R26-loxSTOPlox-L10-GFP mice with ketamine (100 mg kg¹, ip) and xylazine (10 mg kg¹, ip) and stereotaxically unilaterally microinjected 69 nl of the retrograde tracer cholera toxin subunit B (CTB; List Biological, Campbell, CA, USA; 0.2% in saline) into the MCPO/SI (AP: 0.46 mm, DV: 5.3 mm, ML: 1.2 mm) and these animals were then killed 1 week later. …

For the retrograde tracing study, we first surveyed the entire brain using light microscopy. We incubated sections overnight in goat anti-CTB primary antiserum (1:10,000; List Biological Laboratories; catalogue no. 703; lot no. 7032A7) and …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Immunizations and EAE evaluation:

On day 0, mice were immunized subcutaneously with 50 µg of a myelin oligodendrocyte glycoprotein peptide (MOG₃₅₅₅) emulsified 1:1 volume in CFA supplemented with 4 mg/mL Mycobacterium tuberculosis (H37Ra; Difco Laboratories, Detroit, MI, USA). Mice were additionally injected intraperitoneally on days 0 and 2 with 250 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In Vivo Depletion and Transfer of Donor and Recipient Leukocyte Subsets:

Hematopoietic cells were depleted as previously described…by lethal irradiation (2  6.5 Gy) of donor mice 7 days before heart allograft procurement. B6.CD11c-DTR, BALB/c.CD11c-DTR, and CB6F1.CD11c-DTR donors and C57BL/6 (BL/6.CD11cDTR) chimeric heart graft recipients were treated with i.p. 32 ng/g DT (List Biological Laboratories) on days 5, 3, and 1 (donors) or thrice weekly in recipients.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

TT-specific human T cell response:

For in vitro TT stimulation, autologous DCs were co-cultured with CFSE-labeled purified human T cells at different ratios in the presence of 50 ng/ml TT (List Biological Laboratories) for 1014 d.

• Product #190 – Tetanus Toxin from Clostridium tetani