Citations

Animals and EAE induction:

All mice used were aged 6-8 weeks…One hundred nanograms of Pertussis toxin (List Biological Laboratories, CA, USA) was injected intraperitoneally on days 0 and 2 after immunization. EAE was clinically scored daily…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Drugs and Peptides:

DT (50 g/kg, List Biological Laboratories) or vehicle injection was administered via subcutaneous or intramuscular routes in neonates or adult animals (mice), respectively.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… The antitetanus toxoid assay was similarly conducted with the tetanus toxoid (TT) antigen (List Biological Laboratories Inc., Campbell, CA, USA). Sera were titrated against a titration curve of high-titre plasma selected for a reference and given a value of 1000 arbitrary units. …

• Product #191A – Tetanus Toxoid from Clostridium tetani

C. difficile cytotoxin assay:

… A positive control of isolated C. difficile toxin B (List Biologicals, catalog no. 155D) was used. Each well of the cell culture plate was assessed for 80% cell rounding. The cytotoxic titer was determined for each sample by calculating the log₁₀ reciprocal titer per gram of fecal or cecal content.

• Product #155 – Toxin B from Clostridium difficile

… and Toxina A 2 g/ml (List Biological Labs, Campbell …

• Product #152C – Toxin A from Clostridium difficile

Reagents and antibodies:

…CT was purchased from List Biological Laboratories (Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Nasal administration

CT was purchased from List Biological Laboratories (Campbell, CA). … Non-anesthetized mice were nasally administered 30 μg of CT (6 μl of 5 mg/ml, 3 μl per nostril), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

…The amount of piperine in black peppers was quantified as described previously [13]. Cholera toxin (CT) was obtained from List Biological Laboratories, Inc. (Campbell, CA, USA)…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

2.1. Animal model and treatment protocols:

… The murine model of CMA was induced as described previously… In short, mice were sensitized intragastrically (i.g.) with 20 mg whey…
containing 10 µg cholera toxin (CT, List Biological Laboratories, Campbell, CA, USA) as an adjuvant.
Sham-sensitized mice received CT alone. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Stimulation experiments:

Cultured synovial fibroblasts were starved using 0.5% FCS containing medium for 24 hours prior to stimulation with TNF (10 ng/ml), TNF and interleukin-1 (IL-1; 1 ng/ml) (both from R&D Systems), or lipopolysaccharide (LPS) from Escherichia coli (100 g/ml; List Biological Laboratories) for 15 minutes or 24 hours.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/