Bacterial Toxin Research Citations

EAE:

We immunized mice of either gender with myelin oligodendrocyte glycoprotein peptide, amino acids 3555 (MOG₃₅₅₅, 100 µg per mouse, Mimotopes) emulsified in complete Freund’s adjuvant containing heat-inactivated mycobacterium tuberculosis H37RA (8 mg/ml, BD Biosciences), by injecting 25 µl emulsion subcutaneously at each of four sites over the shoulder blades and flanks followed by pertussis toxin (300 ng i.p., List Biological) on 0 and 2 d postimmunization (dpi).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tracer injections:

At the conclusion of the experiment, the retrograde tracers cholera toxin subunit B (CTB; List Biological, Campbell, CA; 12%) and Fast Blue…were injected into area 11 and into area 13, respectively, in one monkey. The reverse was done in a second monkey; CTB was injected into area 13 and Fast Blue into area 11. Both sets of tracer injections were performed unilaterally.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… retrograde Choleratoxin subunit B (CTB) (List Biological Laboratories, CA, USA) … were iontophoretic injected in either DRN …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Pertussis toxin, lyophilized in buffer (List Biological Laboratories Inc.). Resuspend in 1 ml of sterile dH 2O for a concentration of 50 µg/ml and store at 4°C ( see Note 7). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin from Bordetella pertussis (List Biological Labs). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Histochemistry and immunohistochemistry:

Rats were transcardially perfused with room-temperature 0.1 M sodium phosphate buffer, pH 7.3, followed by ice-cold 4% paraformaldehyde in phosphate buffer. Brains were postfixed… Conventional or immunofluorescence protocols were used to reveal the localization of anterograde/retrograde tracers or proteins of interest, following previously described methods (Deutch et al., 1996). For animals receiving nonconjugated CTb injections, a goat anti-CTb antibody (List Biological, Campbell, CA; RRID:AB_10013220) was used.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… In addition, animals received 300 ng of Pertussis toxin by intraperitoneal (ip) route (List Biological Laboratories, Inc., Campbell, CA, USA) on days 0 and 2 post-immunization. Non-immunized (nave) animals were used as control group. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antibodies, reagents and medium:

… CT was from List Biological Labs (Campbell, CA, USA). …

In vivo sensitization, and BLG specific challenge

Mice were sensitized 5 times 1 week apart by oral administration of 10 µg of CT in association with 10 mg of antigen in a solution containing 0.2 M of NaHCO3, pH 9. An oral antigen challenge was performed 1 week after the last sensitization by gavaging 100 mg BLG….

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of casein-mediated food allergy in mice

Female mice were sensitized with 1 mg of casein (Sigma-Aldrich, St. Louis, MO) plus 10 μg of cholera toxin (CT, List Biological Laboratories, Inc., Campbell, CA) by oral administration (p.o.) in 200 μl PBS on days 0 and 7. After this, mice were orally boosted four times for another week, with 5 mg of casein plus 10 μg of CT. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunization protocol for MOG-induced EAE:

…All mice received an intraperitoneal (ip) injection with 150 µl of pertussis toxin (300 ng) (List Biological Laboratories, Campbell, CA) immediately after immunization and again 2 days later. Mice

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)