Bacterial Toxin Research Citations

Materials:

…Lipopolysaccharide (LPS) was obtained from List Biological Laboratories (USA). …

Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza (USA) at passage 1 and maintained in EBM2 Bullet kit growth medium at 37C in a humidified atmosphere of 5% CO2. In all experiments, cells were used at passage 4~9. HUVECs were plated at 90~95% confluence treated for 1 h with 1, 10, and 20 M of quercetin prior to LPS (1 g/mL) stimulation for 1 or 8 h. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… For activation of monocytes, 200-L PBMCs were incubated with 10 g/L ultrapure lipopolysaccharide (LPS) (List Biological Laboratories, Inc, Campbell, CA, USA) in separate 96-well plates. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

IFN- neutralization assay:

The ability of IFN- to upregulate LPS-induced production of TNF- by PBMCs was used to test for the neutralizing activity of sera containing high-titer blocking antibodies to IFN-.

AntiIFN-positive sera, negative control sera, or FBS were preincubated for 30 minutes in a 96-well plate (Corning, Corning, NY) at a dilution of 1:5 in RPMI 1640…or LPS (1 g/mL; LIST Biologicals, Campbell, Calif) alone or both stimuli were added.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Detection of serum IgG antibodies to pertussis antigens:

Nunc MaxiSorp 96-well plates were coated overnight with 0.2 g/ml pertussis toxin (PT), 2 g/ml pertactin (PRN), or 0.2 g/ml fimbriae 2/3 (FIM) in 100 mM carbonate buffer, pH 9.6. Additional plates were coated with 0.5 g/ml filamentous hemagglutinin (FHA) in PBS, pH 7.4. All antigens were purchased from List Biologicals (Campbell, CA). For analyzing antibody responses to whole B. pertussis cells, plates were coated with heat-killed strain D420 prepared as described above. For coating, plates were incubated at 28C for PRN, FIM, and FHA or at 37C for PT and B. pertussis cells. … 

• Product #186 – Fimbriae 2/3 from B. pertussis
• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

Product #170 is currently out of stock.  Large quantities may be obtained through Bulk Requests and Customer Orders.

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

Induction of EAE:

… Mice were anesthetized with 3% isoflurane (Vedco, Inc., St. Joseph, MO) and injected subcutaneously (s.c.) at multiple regions of the back with 100µl (total volume of 300µl) of PLP139151. Intraperitoneal (i.p.) injections of 200ng pertussis toxin (List Biological Laboratories, INC., Campbell, CA) were administered on days 0 and 2 post immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

We temporarily depleted CD207⁺ DCs by injecting diphtheria toxin (DT) into MuLanDTR mice that express transgenic receptors for DT (DTR) in all CD207⁺ DCs³⁵. Compared to untreated MuLanDTR mice, MuLanDTR mice treated with DT for 8 days had reduced percentages of CCR10⁺MHCII⁺ ILCs in sLNs but not in the skin

Diphtheria toxin treatment:

Mice were injected intraperitoneally with 1g diphtheria toxin (List Biological Labs, Inc, Campbell, CA) per week and analyzed 821 days after injection.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

…In addition to 6% OVA, all groups of mice were challenged 3 times a week with 10 ng of Staphylococcus aureus enterotoxin B (SEB; List Biological laboratories, Campbell, Calif) from the 8th through the 14th week after OVA instillation.

Preparation of Antigen and Induction of EAAU:

… In order to induce EAAU, the Lewis rats simultaneously received two injections of different MAA preparations. … (2)MAA was emulsified with 1 µg purified Bordetella pertussis toxin (List Labs,Campbell,CA,USA) and injected intraperitoneally in a total volume of 0.05mL. In the control group, 0.05mL of PBS was simultaneously injected into the left hind foot pad and intraperitoneally in the rats.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… After 30 min, mice were orally immunized by gastric intubation with 1 mg OVA (Grade II, Sigma-Aldrich, St. Louis, MO, USA) in the presence of 10 µg of CT (List Biological Laboratories, Campbell, CA, USA) as the mucosal adjuvant. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reagents and Materials:

Pertussis toxin (PTX) was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). 

Effects of SOD1 on CXCL12-Mediated Cell Migration and G protein coupling:

…like CXCR4, are linked to cell migration towards chemotactic signals. Thus, we tested the effect of the G_i inhibitor pertussis toxin (PTX) on the migration of PC3 cells. In normoxia, PTX treatment abolished migration of PC3 cells (Fig. 7C) while it has less effect on CXCL12-induced chemotaxis in hypoxia (Fig. 7D). The dependence on G_i is confirmed in normoxia (Fig. 7C) using siRNA (validated in Fig. 8) directed against G_i, but not in hypoxic conditions (Fig. 7D). Given the differences observed with PTX treatment, we further investigated the potential role of non-PTX-sensitive G proteins in the migration process as it was previously shown that G proteins G_q and G₁₃ (both PTX-insensitive) may also be involved in CXCL12/CXCR4-dependent migration under some circumstances…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)