Bacterial Toxin Research Citations

EAE Induction:

Pertussis toxin (Cat# 180. 50 µg. List Biological Laboratories, Inc.) was diluted with sterile PBS to 100g/ml.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… encephalomyelitis (EAE). Bordetella pertussis toxin (200 ng, PTX, List Biological Laboratories, Campbell, CA) was administered intra-peritoneally on the same day and 2 days after immunization to enhance the autoimmune response. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Active EAE induction:

Female WT or Cav-1 KO mice were subcutaneously immunized with 200 µg MOG₃₅₅₅ in complete Freund’s adjuvant …Pertussis toxin (200 ng, List Biological Laboratories) was injected intraperitoneally twice on day 0 and 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE immunization and clinical evaluation:

For active EAE, mice were immunized subcutaneously with 175 µg MOG_35-55 (Anaspec) emulsified in Incompete Freunds Adjuvant (Sigma-Aldrich) supplemented with 0.4 mg Mycobacterium tuberculosis H37 (Difco Laboratories) on day 0 and given 200 ng pertussis toxin (List Biologicals) intraperitoneally (IP) on day 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…rPA was obtained from List Biological Laboratories, Inc. The assay endpoint is reported as the serum mean concentration of anti-PA specific IgG (g/mL). … viability. rPA and rLF were obtained from List Biological Laboratories, Inc. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Induction and assessment of EAE:

…For disease induction, mice were immunized subcutaneously in both sides of the flank with 200 µg MP4 (stock: 2 mg/ml) in CFA. Control mice remained untreated. On the day of immunization and 48 h later, 200 ng pertussis toxin (PTX; List Biological Laboratories, Hornby, ONT, Canada) were given in 500 µl sterile phosphate-buffered saline (PBS). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

ON Injury

ON crush surgery and intraocular injections were performed under general anesthesia. The mice received either 6.4 μg or 25 μg of zymosan 5 days prior and immediately after ON crush into the vitreous. At 5 days prior to sacrifice, CTB (3 μL, 1%; 103B, List Biological) was injected as an anterograde tracer. For C57BL/6 control n = 5; 6.4 μg n = 4; 25 μg n = 5. For CAST/Ei control n = 4; 6.4 μg n = 4; 25 μg n = 6. Quantification was performed blinded to the treatment and mouse strain.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

DT preparation and administration:

DT (List Biological Laboratories Inc., catalog 150) was administered to NSG-DTR mice in a single, 300 l i.p. injection of 0.5, 1.0, 2.5, 5.0, 10, or 25 ng total DT. Control NSG-DTR mice (PBS) were treated with an equal volume…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental design:

Sensitization and challenge of mice were performed as described by Lpez-Expsito et al.17 BALB/c and C3H/HeOuJ mice (5 per group) were sensitized once per week for 6 weeks, by gavage with 2 different doses of OVA-LPS-free (1 and 5 mg) dissolved in 0.5 mL of 0.2 M bicarbonate with 10 µg of cholera toxin (CT) (List Biologicals, Campbell, CA, USA). Nave mice received 10 µg of CT in 0.5 mL of bicarbonate. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials:

…Azide-free CT from V. cholere Inaba 569B was obtained from List Biological Laboratories (Campbell, CA). …

cAMP assay:

Caco-2 cells (7.0 × 105 cells/2 mL medium) were seeded in 6-well plastic plates. One week later, the cells were washed twice with PBS and exposed for 30 min–2.5 mM HEPES buffer containing 0.5 µg/mL of GM1 or in the absence of GM1. Subsequently, the cells were washed twice, and then CT and 1 mM IBMX were added to the cultured cells in 2 mL of 0.01% BSA in 2.5 mM HEPES buffer containing peptide P3 (50 nM/mL or 50 µM) or in the absence of P3. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae