Bacterial Toxin Research Citations

… by subcutaneous injection of 50 µg of MOG 3555 in Freund’s Complete Adjuvant (Thermo Fisher Scientific, Rockford, USA) supplemented with 4 mg/ml of Mycobacterium tuberculosis on day 0. Intraperitoneal pertussis toxin (0.1 µg/200 µl; List Biological Laboratories, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…containing 500 µg of Mycobacterium tuberculosis H37RA (Difco, Detroit, USA). Immediately after immunization and again 48 h later, the mice received a tail vein injection of 200 ng of Pertussis toxin (List Biological Laboratories, CA, USA) in 100 µl of PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and KYC treatment:

For MOG-induced EAE, mice on the C57BL/6 background were subcutaneously immunized with 200 µg MOG₃₅₅₅ peptide emulsified in an equal volume of complete Freund’s adjuvant…containing 4 mg/mL mycobacterium followed by i.p. injection of 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) on days 0 and 2, as described…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…EAE was induced by subcutaneous immunization with 100 µg of the MOG 3555 peptide (MEVWYRSPFSRVVHLYRNGK; Mimotopes) emulsified in CFA…and two intravenous injections of PTx (day 0, 200 ng; day 2, 400 ng; List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Chemicals:

… SNAPtide (FITC/DABCYL) peptide (a labeled peptide that is a substrate for BoNT/A) and Type A Light Chain (LcA) were purchased from List Biological Laboratories, Inc. (Campbell, CA). …

Detection of Surrogate for BoNT/A:

The SNAP-25 assay, as described by Sapsford et al. (2008), was used to detect the enzymatically active light chain A (LcA) portion of BoNT/A, and served as a surrogate for the entire toxin. …

• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin
• Product #610A – Botulinum Neurotoxin Type A Light Chain, Recombinant

Characterisation of the model:

Podo-DTR mice and wild-type littermates as controls were injected with a single dose of diphtheria toxin (DT) (Product no. 150, Lot 15023A1; List Biological Laboratories Inc., California, USA) ranging from 0.1166ng/g body weight (bw). …

ACEi protection experiment:

Groups of 16 transgenic (Tg) mice and eight wild-type (WT) littermates were given captopril (200mg/L) (Product no. C4042, Sigma Aldrich, UK) in their drinking water or placebo (water alone) 24h after a single intraperitoneal (i.p.) injection of diphtheria toxin at 5ng/g bw (Product no. 150, Lot 15023A1; List Biological Laboratories Inc., California, USA). …

Urine and blood analysis:

Urine and serum creatinine concentrations were measured using the creatinase reaction, with the exception of the first cohort of the model evaluation studies where Jaffe reaction was used (for the Podo-DTR line 47 given 1ng/g DT).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Intracellular Cytokine Staining:

PBMCs collected from chronically rhCMV-infected rhesus monkeys stimulated with 10 g/mL Staphylococcus aureus enterotoxin type B (List Biological Laboratories) were included in each experiment as a positive control.

Experimental autoimmune encephalomyelitis:

EAE was induced in 8-10 week old C57BL/6 female mice using a previously established protocol by Miller… Pertussis toxin (200 ng) (List Biological Laboratories Inc., Campbell, CA) was injected intraperitoneally on the day of and 48 h following immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Study Groups:

We studied two groups of 6 supine mechanically ventilated sheep. …During this ventilation period, sheep received a continuous intravenous infusion of endotoxin (10 ngkg^-1min^-1, Escherichia coli O55:B5, List Biological Laboratories Inc, California).

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

When cells were treated with levosimendan or pimobendan, these agents were added 30 minutes before the challenge with lipopolysaccharide (LPS; Escherichia coli 055:B5; List Biological Laboratories, Campbell, CA).

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5