Bacterial Toxin Research Citations

Cell culture and transfection:

The breast cancer cell line MCF-7 and the normal breast cell line MCF-10A, …and cholera toxin (List Biological Laboratories, Inc., Campbell, CA, USA). MCF-7 was grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (FBS). All cells were cultured on the surface of a 75-cm2 culture flask. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Eight-ten week old male wild-type (WT) C57BL/6JBomTac (Taconic, Skensved, Denmark) and syndecan-4 knock-out (KO) mice [20] were randomized to intraperitoneal injection with a single dose of LPS (10 mg/kg, List Biological Laboratories Inc., Campbell, CA) dissolved in …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Animals and EAE induction:

Female C57BL/6 mice averaging 8 weeks of age were obtained from Dooyeol Biotech (Seoul, Korea). …EAE was induced by subcutaneous immunization in C57BL/6 mice with 50 µg MOG₃₅₅₅ (peptide MOG; …In addition, 300 ng pertussis toxin (List Biological Laboratories, CA, USA) was administered by intravenous injection on days 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Un-nicked Diphtheria Toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA) and was used to deplete Foxp3+ Tregs from DEREG mice (Lahl et al., 2007) by administering 1g i.p. DT per 2 days from day -2 to day 6 p.i. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Tracer injections:

Rats were given intraperitoneal injections of a mixture of 45% ketamine (100 mg/ml), 35% xylazine (20 mg/ml) and 20% saline at a dose of 0.16 ml/100 g body weight, and several minutes later were placed into a Kopf stereotaxic instrument. Small bore holes were drilled in the skull above targeted structures and 1% Fluorogold (FG, Fluorochrome, Inc, Englewood, CO) in 0.067 cacodylate buffer or 10% subunit of cholera toxin (Ct; List Biological Laboratories Inc., Campbell, CA) in 0.1M Sorenson’s phosphate buffer (SPB; pH 7.4) were injected iontophoretically into the brains from 1.0 mm filament-containing borosilicate glass pipettes pulled to outside diameters of 15-18 m (for FG) or 20-25 m (for Ct). The injections were made over a period of 15 min using positive pulses (7s on/7s off) of 1 A (for FG) or 3 A (for Ct). Animals were given subcutaneous (s.c.) injections of carprofen (20 mg/kg) immediately after surgery and once daily for the next 3 days. Stereotaxic coordinates were established with the aid of the atlas of Paxinos and Watson (1997). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Experimental autoimmune encephalomyelitis:

Mice on the B6 background were immunized subcutaneously with 100µg MOG_3555…Mice received 300ng pertussis toxin (List Biological Laboratories) intraperitoneally on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:  cholera toxin B (Ctb; List Biological Laboratories)

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Saltl>

… 100 ng/ml pertussis toxin (PTX) (List Biological Laboratories, Campbell …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell cultures, bacteria and reagents:

…Lipopolysaccharide (LPS) from E. coli O111:B4 was from List Biological Laboratories (Campbell). …

Macrophage cell challenge and cytokine determination:

…Macrophages were also treated with cell-free bacterial spent culture supernatant (SCS; 1/25 dilution), LTA (10 g/ml) and LPS (100 ng/ml) in c-RPMI for the time indicated. …

• Product #201 – LPS from Escherichia coli O111:B4

Animal manipulations:

… Active EAE was induced in C57BL/6 mice by s.c. immunization with 100 µg myelin oligodendrocyte glycoprotein (MOG)₃₅₅₅ peptide (Biosynthesis) emulsified in CFA supplemented with 4 mg/ml heat killed Mycobacterium tuberculosis, H37Ra. Each C57BL/6 mouse also received 300 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) via intraperitoneal (i.p.) injection on the day of immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)