Citations

Recording Solutions and Drugs:

… In one set of experiments, the CSMG neurons were pretreated with pertussis toxin (PTX, List Biological Laboratories, Campbell, CA) overnight (12-16 hr). PTX was added to the culture medium at a final concentration of 500 ng/ml. At this concentration, PTX has been shown to selectively uncouple G_i/o G proteins from GPCR [22].

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

…pertussis toxin from LIST Biological Laboratories, Inc. (Campbell, CA, USA), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and therapeutic study:

SJL/J (H-2s) female mice, 46 weeks old, …The PLP139151/complete Freunds adjuvant was administered to regions above the shoulders and the flanks (total of four sites; 50 µl at each injection site). In addition, 200ng/100 µl of pertussis toxin (List Biological Laboratories, Campbell, CA) was injected intraperitoneally (i.p.) on the day of immunization (day 0) and 2 days postimmunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… intraperitoneally with 200 ng of pertussis toxin (List Biological Laboratories, Campbell…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Autoimmune Encephalomyelitis (EAE):

EAE was induced as previously described [24,35]. Briefly, mice were immunized subcutaneously with 150 µg of MOG₃₅₅₅ (Pepceuticals, Leicestershire, UK) emulsified in CFA (BD Difco, Heidelberg, Germany) and received 200 ng pertussis toxin (PTx, List Biological Laboratories, Inc., Campbell, CA, USA) intraperitoneally at the time of immunization and 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Protective Antigen (PA) was from List Biological Laboratories, Campbell, CA, USA. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

EAE:

…Mice also received intraperitoneal injections of pertussis toxin (300 ng in 100 µl PBS; List Biological Lab, Campbell, CA, USA) at the time of immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…In six rats, BDA was injected into the MPB (n = 3) or MPBE (n = 3) as described above, and 0.5% cholera toxin B subunit (CTb; List Biological Laboratories, Campbell, CA, USA) in phosphate buffer was injected unilaterally into the CPu by iontophoresis (positive, 4 A, 2 Hz, 15 …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE Model:

Our EAE model was designed with reagent concentrations that produce a mild-to-moderate form of EAE, always yielding clinical scores of motor dysfunction below 3 and 4 over the 4 week testing period [34]. All animals in the current study exhibited neither paresis of both hindlimbs nor paralysis of one hindlimb, thus allowing nociceptive paw reflex testing. … MOG was bilaterally injected (150 µg/100 µl s.c.) at the flanks under light isoflurane anesthesia on Days 0 and 6. Pertussis toxin (List Biological Laboratories, Campbell, CA) was injected (200 ng/200 µl, i.p.) on days 0 and 2 to facilitate opening of the blood brain barrier to T cells by histamine-induced vascular leakage and/or the priming of autoreactive T cells [16]. Control mice received CFA and pertussis toxin, but not MOG. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Blocking neutrophil migration from skin:

Microbial inflammation was induced as above, and, 4h later, ear pinnae of photoconvertible mice were injected using a 29-gauge needle with 5 µg of anti-CD11b, (clone M1/70), …or 500ng of pertussis toxin (List Biological Laboratories) or PBS control, immediately before photoconversion.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)