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January 1, 2011
Human Vaccine
Mett V, Chichester JA, Stewart ML, Musiychuk K, Bi H, Reifsnyder CJ, Hull AK, Albrecht MT, Goldman S, Baillie LW, Yusibov V
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
In vitro characterization of plant-produced mAbs:
ELISA was performed as previously described in reference 36, with slight modifications. Plates were coated with 1 g/mL of recombinant B. anthracis PA (List Biological Laboratories Inc., Campbell, CA). …
April 12, 2010
The Open Vaccine Journal
Li Q, Peachman KK, Sower L, Leppla SH, Shivachandra SB, Matyas GR, Peterson JW, Alving CR, Rao M, Rao VB
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Protein Purification:
Highly purified recombinant proteins were prepared in our laboratories for binding assays as well as immunizations, as described below. For ELISA and LTx neutralization assays, purified PA and LF were purchased from List Biologicals Laboratories, Inc., Campbell, CA. …
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
August 1, 2009
Infection and Immunity
Boyer AE, Quinn CP, Hoffmaster AR, Kozel TR, Saile E, Marston CK, Percival A, Plikaytis BD, Woolfitt AR, Gallegos M, Sabourin P, McWilliams LG, Pirkle JL, Barr JR
Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis
LF quantification:
… To achieve the necessary dynamic range and to account for high-sensitivity rapid-response sample analysis, two standard curves were prepared, one for 20 ng/ml, l (low volume [LV]) and the other for 200 ng/ml, l (high volume [HV]) serum volumes. In addition, three quality control samples (QCs) were prepared in human serum pools (Interstate Blood Bank, Memphis, TN) using recombinant LF (List Biological Laboratory, Campbell, CA) and validated. …
PA antigen capture immunoassay:
Microtiter plates (Thermo Labsystems, Franklin, MA) were coated with monoclonal anti-PA antibody AVR1046 obtained by the same methodologies used for anti-LF monoclonal described previously (4) at 1.28 g/ml in 0.01 M phosphate-buffered saline (PBS), pH 7.4. Coated plates were washed with a wash buffer consisting of PBS and 0.1% Tween 20, pH 7.4. Recombinant PA (rPA; List Biological Laboratories, Inc., Campbell, CA) was serially diluted twofold using a 1/25 or a 1/100 dilution of normal rhesus macaque serum (RMS). The rPA dilutions formed a seven-point standard curve from 0.6 to 37.5 ng/ml. …
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
March 1, 2009
Antimicrogial Agents and Chemotherapy
Sharma S, Thomas D, Marlett J, Manchester M, Young JA
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
We next tested the ability of RDI to neutralize LeTx containing an antibody-resistant form of PA (PAL685A) in a rat model system. Male Harlan Sprague-Dawley rats (180 to 200 g; Harlan, Indianapolis, IN) were anesthetized with isofluoranes and inoculated with 500 l LeTx mixture by intravenous injection in the tail vein. The anthrax LeTx was prepared for each group by mixing 12 g LF (List Biological Laboratories, Campbell, CA) with 40 g PA (List Biological Laboratories, Campbell, CA) …
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
February 1, 2009
Molecular Therapy
Liu TH, Oscherwitz J, Schnepp B, Jacobs J, Yu F, Cease KB, Johnson PR
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
For the procurement of positive control hyperimmune anti-PA and anti-LF serum for use in the western blotting and as positive controls for the LeTx neutralization assays, rabbits were immunized with 250 g of either soluble PA83 or LF in an emulsion with CFA (List Biological Laboratories, Campbell, CA). Rabbits were then boosted three times at 23-week intervals with 125 g of the respective immunogens in an emulsion with incomplete Freund’s adjuvant …
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
September 3, 2008
Plos One
Gupta PK, Moayeri M, Crown D, Fattah RJ, Leppla SH
Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis
Samples of LF used for comparison were purchased from List Biological Laboratories (Campbell, CA).
Results – Toxicities of LF proteins produced from avirulent B. anthracis strains:
… B. anthracis strains cured of the pXO1 and pXO2 virulence plasmids [18]. LF expressed from pSJ115 is here termed LF-HMA to denote the presence of the two residues His-Met (HM) added at its N-terminus due to the cloning manipulations. This expression system is licensed to List Biological Laboratories (Campbell, CA), and the LF sold by them is therefore also LF-HMA. …
February 9, 2008
Proceedings Of The National Academy Of Sciences Of The United States Of America
Bolcome, RE III; Sullivan, SE; Zeller, R; Barker, AP; Collier, RJ; Chan, J
Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis
Microinjection of Toxin Proteins and Use of Inhibitors:
… LF was purchased from List Laboratories, Inc. …
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
October 5, 2007
Plos Pathogens
Manayani DJ, Thomas D, Dryden KA, Reddy V, Siladi ME, Marlett JM, Rainey GJ, Pique ME, Scobie HM, Yeager M, Young JA, Manchester M, Schneemann A
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Quantification of PA83 bound to chimeras 206 and 264:
Recombinant PA83 (List Biological Laboratories) in 5 mM Hepes, 50 mM NaCl (pH 7.5) was mixed with purified chimeras 206 and 264 in 50 mM Hepes (pH 7.5) in a ratio of 180:1 (equimolar amounts of PA83and VWA domains). Following incubation for 20 min at room temprature, an aliquot from each of the samples was removed and stored at 20 C pending analysis. The remainder of the samples was transferred to an ultracentrifuge tube and underlayed with a 30% (w/w) sucrose cushion in 50 mM Hepes (pH 7.5). Complexes of chimeras decorated with PA83 were pelleted by centrifugation at 200,000g for 45 min. …
August 15, 2007
Bioorganic & Medicinal Chemistry
Karginov VA, Nestorovich EM, Schmidtmann F, Robinson TM, Yohannes A, Fahmi NE, Bezrukov SM, Hecht SM
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
…Recombinant B. anthracis lethal factor (rLF) and protective antigen (rPA) were acquired from List Biological Laboratories, Inc. (Campbell, CA) …
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
February 1, 2007
List Labs POSTER - Presented at the 5th Annual ASM Biodefense and Emerging Diseases Research Meeting, February 2007 in Washington, D.C.
Shine, N., Crawford, K.
Product: SNAPtide® Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin
The following FRET peptides have been designed at List Biological Laboratories as substrates for the botulinum toxin enzymes. SNAPtide, Product #520 and #521 (U.S. Patent, No. 6,504,006 ) is readily recognized and cleaved by the Botulinum toxin type A (BTA), Product #130, and the BTA light chain (LcA), Product #610A. One of the substrates, Product #520, contains an oAbz/DNP FRET pair and the other, Product #521, a FITC/DABCYL FRET pair. Representative data are given below. VAMPtide, Product #540 is readily recognized and cleaved by the Botulinum toxin type B light chain (LcB), Product #620A. This FRET substrate contains an oAbz/DNP FRET pair. SNAP Etide, Product # 550 is readily recognized and cleaved by the Botulinum toxin type E light chain (LcE), Product #635A. This FRET substrate contains an oAbz/DNP FRET pair.
(LF) is the enzymatic component of anthrax lethal toxin which specifically cleaves the MAPK-kinase proteins. LF is also an ideal target for therapeutic inhibitors. FRET substrates for LF, MAPKKide, Product #530 and 531, have been designed at List Biological Laboratories. One of the substrates, Product #530, contains an oAbz/DNP FRET pair and the other, Product #531, a FITC/DABCYL FRET pair. Product #531 is especially well suited for high throughput screening for the IC50 and inhibitor modality of potential inhibitors. …
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