Bacterial Toxin Research Citations

… To assess the potential of DNI and DNI conjugate as a vaccine against B. anthracis, we investigated whether the vaccinations protect against an injection of LeTx. Three weeks after the last vaccination, each mouse was injected into the tail vein by a mixture of 48 g pa and 20 g LF (list biologicals, Campbell, CA). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

rPA Delivery in Rats:

Recombinant protective antigen from Bacillus anthracis (rPA) was obtained from List Biologicals (Campbell, CA). The MSA containing rPA was fabricated using a casting and drying fabrication method. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Anti-PA and anti-LF IgG and IgA antibody titers:

Anti-PA and anti-LF immunoglobulin G (IgG) titers in mouse serum samples were determined by enzyme-linked immunosorbent assay (ELISA) using purified PA or LF protein (List Biological Laboratories, Campbell, CA) as the capture antigen. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Materials: 

… PA toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). …

Cloning:

In the ACE process the aptamers were initially labeled with a 6-carboxyfluorescein and incubated with increasing concentration of PA Toxin. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Recombinant Protein ELISAs:

Ninety-six well plates were coated overnight at 4 C with 1 g/well of rLF or rPA (List Biologicals, Campbell, CA). After washing with PBS-Tween and blocking with PBS/BSA, diluted pre-immune and Day 28 (4 days after a second booster) serum from each mouse was added in duplicate and incubated for 2 h at room temperature (RT). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Challenge with anthrax toxin or MEK pathway inhibitors:

At indicated time points, cultured cells were treated with fresh medium containing 2 g/ml PA or 2 g/ml LT (2 g/ml PA plus 2 g/ml LF), both from List Biological Laboratories, Inc., Campbell, CA. An equal volume of diluent was used as a negative control. Duration of treatment was 24, 72, or 144 h or as indicated. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Measurement of cytotoxicity of diphtheria and anthrax toxin:

… To determine the protective effect of the purified nanobodies on anthrax toxin cytotoxicity, RAW cells were pre-incubated with 20 M purified nanobodies or 10 M D9R in 48-well plates (Costar) for 30 min at 37 C. Subsequently, 200 ng/ml PA (protective antigen; List Biological Labs) and 400 ng/ml LF (lethal factor; List Biological Labs) were added and the cells were incubated for 1.5 h, with one medium change after 45 min. The cell viability was determined using the MTT assay described above.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Expression and Labeling of M18 scFv :

A standard 3-ml culture of cells harboring plasmids to surface display M18 scFv (e.g. pBAD_M18_138) was grown in LB medium (BD Diagnostics) in the presence of 30 g/ml chloramphenicol (Thermo Fisher Scientific) to an optical density (A₆₀₀) of 0.6 at 25 C. Cells were then induced via the addition of 0.2% l-arabinose (Sigma) to express M18 scFv for 12 h at 25 C. The presence of M18 scFv on the surface of E. coli was characterized by the ability of intact cells to bind antigens (PA₆₃ and PAD4) using flow cytometry. PAD4 containing FLAG epitope tag (rPAD4) was recombinantly expressed in E. coli and purified as described previously (35), and PA₆₃-FITC was obtained from List Biological Laboratories, Inc. …  

• Product #175 – Anthrax PA 63 – FITC Conjugate                               

For Western blots, whole-cell lysates from 106 J774A.1 cells, separated by SDS/PAGE, were probed by anti-PA (List Biological Laboratories) and anti-actin antibodies (Santa Cruz Biotechnology) with chemiluminescence detection. …

 Materials:

The dominant negative inhibitor antigen was produced by Pharmaceutical Solutions LLC
(Bloomington, IN) as a lyophilized powder in sealed glass vials. The vials of the DNI protein
contained 25 mg of protein, 113 mg mannitol, 33 mg sucrose, and 2.4 mg dibasic sodium
phosphate. They were reconstituted with 3.3 ml of water for injection (WFI), the
reconstituted protein concentration was 91 M . 10 mg of recombinant protective antigen was
also purchased from List Biological Laboratories Inc (Campbell, CA). …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis