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July 1, 2013
Clinical And Vaccine Immunology
Lu, H;Catania, J;Baranji, K;Feng, J;Gu, M;Lathey, J;Sweeny, D;Sanford, H;Sapru, K;Patamawenu, T;Chen, JH;Ng, A;Fesseha, Z;Kluepfel-Stahl, S;Minang, J;Alleva, D;
Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis
Production and purification of rPA and rLF preparations:
| rLF-HMAL | List Biologicals (1722BB) |
MLA:
The J774A.1 mouse macrophage cell line (ATCC; TIB-67, Manassas, VA) was cultured in 75-cm2 flasks in Dulbecco’s modified Eagle’s medium with high glucose … Culture medium was discarded before incubation with rPA and rLF. The macrophage lysis assay (MLA) using different concentrations of rPA and rLF was performed by diluting reagents in appropriate volumes of complete DMEM.
June 27, 2013
Immunity
Yan, Y;Jiang, W;Spinetti, T;Tardivel, A;Castillo, R;Bourquin, C;Guarda, G;Tian, Z;Tschopp, J;Zhou, R;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Reagents:
Lethal Toxin, consisting of protective antigen and lethal factor, was from List Biological Laboratories.
…cells used for the Lethal Toxin stimulation were from BALB/c mice.
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
June 11, 2013
PNAS
Slater, LH;Hett, EC;Clatworthy, AE;Mark, KG;Hung, DT;
Product: Anthrax Edema Factor (EF), Recombinant from B. anthracis
ET Assays.
EF was obtained from List Biological Laboratories. J774A.1 cells in 384-well plates were incubated with 11 nM PA and 3.7 nM EF for 5 h at 37 C, and the concentration of cAMP was measured by cAMP enzyme immunoassay…
Author did not specify which List Labs Anthrax Edema Factor was utilized. List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).
June 6, 2013
PLoS ONE
Thullier, P;Avril, A;Mathieu, J;Behrens, CK;Pellequer, JL;Pelat, T;
Product: Anthrax Edema Factor (EF), Recombinant from B. anthracis
ELISA and western blot:
The cross-reactivity of scFv 2LF with EF was tested by ELISA. Briefly, microtiter plates were coated with 2 g/ml of EF (List Biological Laboratories, Campbell, CA), while LF (List biological Laboratories) and KLH (Sigma-Aldrich, Saint-Quentin Fallavier, France) were utilized as positive and negative controls respectively. …
Affinity measurements:
Affinity constants (KD) and dissociation constants (kd) of scFv 2LF for LF, EF and their variants were measured by surface plasmon resonance (SPR) with a Biacore X (Biacore, Uppsala, Sweden) instrument. …
Author did not specify which List Labs Anthrax Edema Factor was utilized. List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
June 1, 2013
Nature Chemical Biology
Hett, EC;Slater, LH;Mark, KG;Kawate, T;Monks, BG;Stutz, A;Latz, E;Hung, DT;
Product: Anthrax Edema Factor (EF), Recombinant from B. anthracis
Reagents were purchased as follows: edema factor (LIST Biologicals)
Author did not specify which List Labs Anthrax Edema Factor was utilized. List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).
May 20, 2013
Journal Of Biotechnology
Lee, YJ;Kim, HS;Ryu, AJ;Jeong, KJ;
Product: Anthrax Protective Antigen, Activated (PA 63) from B. anthracis
…For ELISA, 50 L of a 5 g/mL solution of anthrax PA, activated PA63 (List Biological Labs, Campbell, CA, USA), was dissolved in carbonate-bicarbonate buffer and used to coat each well of a 96-well plate …’
May 1, 2013
Protein Expression And Purification
Liu, J;Cai, C;Guo, Q;Zhang, J;Dong, D;Li, G;Fu, L;Xu, J;Chen, W;
Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis
… A commercially available rLF used as control was purchased from List Biological Laboratories. …
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
April 19, 2013
ACS Chemical Biology
Slater, LH;Hett, EC;Mark, K;Chumbler, NM;Patel, D;Lacy, DB;Collier, RJ;Hung, DT;
Product: Anthrax Edema Factor (EF), Recombinant from B. anthracis
Cells, antibodies and reagents:
…EF was obtained from List Biological Laboratories.
ET assay:
5 104 J774A.1 cells in 100 L were plated in 96-well plates, and incubated overnight. Cells were preincubated with compound for 2 hours, before the addition of ET (11 nM PA, 1.2 nM EF) for 5 hours at 37 C. The concentration of cAMP in cell lysates was measured by cAMP enzyme immunoassay…
Author did not specify which List Labs Anthrax Edema Factor was utilized. List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).
April 1, 2013
Archives Of Pharmacal Research
Wui, SR;Han, JE;Kim, YH;Rhie, GE;Lee, NG;
Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis
… PA and LF used for the toxin neutralization assay were purchased from List Biological Laboratories. …
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
March 15, 2013
Veterinary Immunology And Immunopathology
Byrd, W;Boedeker, EC;
Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis
CFA/I and LT Antigens:
The CFA/I fimbriae were isolated as previously described (Byrd et al., 2003) and the LT was obtained from List Biological Laboratories (Campbell, CA).
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
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