Bacterial Toxin Research Citations

… and PA was obtained from List Biological Laboratories (Campbell, CA, USA). …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Reagents:

For the bilayer lipid measurements, PA 63 was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA).

Channel Reconstitution into Planar Lipid Bilayers:

…Single channels were formed by adding 0.5 to 1 L of 20g mL 1 solution of PA63, or 0.2 to 0.5 L of 48 ng mL 1 solution of C2IIa to the 1.5 mL aqueous phase on the cis-half of the bilayer chamber. For multichannel experiments, we applied 12 L of 0.2 mg mL1 stock PA63 or 12 L of 48g mL 1 stock C2IIa to the cis-side of the membrane. Under this protocol, PA63 and C2IIa channel insertions were always
directional as judged by channel conductance asymmetry in the applied transmembrane voltage…In most of the experiments, the PAMAM dendrimers were added to the cis-compartment of a bilayer chamber, which was the side of PA63 and C2IIa addition.

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

General reagents:

…Anthrax PA83 was purchased from List Biological Laboratories (Campbell, CA, USA). …

MPII proteolysis of proteins in vitro:

Anthrax PA83 (2 g; ~ 1 m) and the following purified human proteins the furin 27107 prodomain (1.8 g, ~ 9 m) [20], the MT1MMP proenzyme (2 g; ~ 2 m), MBP (2 g; ~ 5 m) and BTN3A2 (2 g; ~ 2.5 m) were each coincubated for 1 h at 37 C with increasing concentrations of MPII in 50 mm Hepes, pH 8.0, containing 1 mm CaCl₂, 0.5 mm MgCl₂ and 10 m ZnCl₂. As a control, PA83 and the MT1MMP proenzyme were coincubated for 1 h at 37 C with furin (at the 1 : 100 to 1 : 10 000 enzyme/substrate molar ratio) in 20-L reactions containing 100 mm Hepes, pH 7.5 and 1 mm CaCl₂. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Materials:

… Activated protective antigen [63 kDa (1 M=63g/ml); PA63] and recombinant lethal factor [90 kDa (1 M=90 g/ml); LF] were obtained from List Biological Laboratories (Campbell, CA). …

PA63 and LF were used in the following:

• Optimization of LTx complex detection assay
• Limit of detection and quantification
• Precision and Accuracy for PA83 and PA63 Detection
• Detection of PA63 and LTx in the presence of AIG

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Cytotoxicity assay:

PA and LF were purchased from List Biological Laboratories, Inc., …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Macrophage isolation, culture and transfection:

Cells were transfected with poly(dA:dT) (2 ng/mL; Sigma), MDP (20 ug/mL; Invivogen), Salmonella thyphimurium flagellin (140 ng/mL; Invivogen) and Bacillus anthracis Lethal toxin and protective antigen (1 ug/mL; List Biological Laboratories).

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

The Anthrax Lethal Factor was obtained from List Biological Laboratories, INC.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

BA spores and antigens:

… BA PA and LF were purchased from List Biological Laboratories, Inc. (Campbell, CA, USA).

Primary and secondary T-cell responses to BA:

The in vitro human T-cell assay was adapted from standard assays conducted for the study of human immune responses… To assess secondary recall T-cell responses to BA, cells from the 7-day co-culture were harvested, washed, counted, and replated at a hDC:TC ratio of 1:10 in 24-well plates for 48 h with a second preparation of autologous hDCs that were infected with BA spores (WT or gerh) at a MOI of 1:1, 2.5 g/mL PA, 1 g/mL LF, KLH (10 g/mL), or left untreated.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Reagents:

Lyophilized recombinant PA and LF were purchased from the List Biological Laboratories, Inc. (Campbell, CA), and reconstituted in sterile water to make stock solutions with final concentrations of 1 mg/ml.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Gene Expression Profiling:

RNA was isolated from HepG2 cells cultured under normoxic or hypoxic conditions with or without LT using TRIzol according to…

Polysome Analysis:

Polysome analysis was performed as described previously (37). In brief, HepG2 cells were pretreated with or without LT for 3 h and then cultured under hypoxic conditions for 4 h. …

Cytotoxicity Assay:

HepG2 and Hepa1c1c7 cells were cultured in 96-well plates with or without LT either 3 h prior to or at the initiation of a 24-h incubation under hypoxic conditions. …

Immunizations and serum isolation:

Groups of eighteen (18) female C57Bl/6 (National Cancer Institute/Charles River Laboratories, Wilmington, MA) mice at 8-12 weeks of age were subcutaneously immunized with saline, 5 g recombinant anthrax protective antigen (rPA; List Biological Laboratories, Inc., Campbell, CA) alone or with 1.3 mg alum (Alhydrogel; Sigma, St. Louis, MO). On day 71 post-immunization, three mice from each group were given a boost of rPA (no adjuvant) at the same dose as the primary immunization …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis