Bacterial Toxin Research Citations

… Protective Antigen (PA) was from List Biological Laboratories, Campbell, CA, USA. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Proteins and peptides:

Immobilized PA83 (LIST Biological Laboratories, Inc.) was used in the ELISA to establish titers of PA-reactive Ab in the clinical samples. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Mice were injected in both thigh muscles with 50 L of a formulation containing…or 20 g/mL anthrax recombinant protective antigen (rPA) (List Biological Laboratories, Campbell, CA)…

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Reagents:

…LF, EF, PA83, and PA63 were obtained from List Biological Laboratories (Campbell, CA). …

Animal care and study protocol:

…Total LF and LTx analysis was determined in two animals, 16N and 89N, over the course of infection. …

Antibody specificity:

Specificity of anti-PA and anti-LF MBs was demonstrated for LTx, LF, and PA. PA63/LF were mixed at a w/w ratio of 10:1 to form LTx complex. PA83 and LF were mixed as a non-complexed control. Mixtures were incubated at room temperature (RT) for 30 min. LF, PA63, PA83, LF/PA63, and LF/PA83 at 0.5 g LF and 5 g PA83 or PA63 were immunoprecipitated with anti-LF and anti-PA MBs, …

Lethal toxin standard preparation and characterization:

LTx was formed by mixing PA63 and LF at w/w ratios of 0:1 to 60:1 PA63/LF, …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

Mouse anti-CFA and anti-toxin antibody titration:

Anti-CFA/I and anti-CS1, -CS2, -CS3, -CS4/CS6, -CS5/CS6, and anti-STa and anti-LT IgG antibodies in serum of each mouse were titrated as described previously [34,3739]. Briefly, 500 ng CFA/I, CS1, CS2, CS3, CS4/CS6, and CS5/CS6 heat-extracted from ETEC field isolates or recombinant E. coli strains [39] (Table 1), or 100 ng LT (List Biological Laboratories, Inc., Campbell, CA) were coated to each well of H2B plates …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Cells and Reagents:

…Polyclonal goat antibody (#771B) against Protective Antigen (PA) was from List Biological Laboratories (Campbell, CA) and used at 1/2000 dilution…

Cell Preparation, Reagents, and Stimulation:

In some experiments, cells were pretreated with Ac-YVAD-cmk (50 M, ALX-260028-M001, Enzo Life Sciences) for 30 min before S. Typhimurium infection or fliC transfection. Otherwise, cells were treated with anthrax protective antigen (1 g/mL, List Biologicals)…

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… For the rPA, 7.5 g of rPA (LIST Biological Laboratories) was mixed with alum and either saline or 50 g of Poly I:C was added to the mix on the day of immunization. Antibody assays. To measure levels of antibodies to the rPA …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Animals and vaccinations:

For rabbit experiments employing Freunds adjuvant, Female New Zealand white (NZW) rabbits (Covance Research Products, Denver, PA) weighing approximately 2.5 kg were immunized on day 0 with a mixture of two MAPs (125 g per MAP) in CFA and boosted 4 times at two-week intervals with the MAPs in IFA. For studies using human-use adjuvants, NZW rabbits were injected with TB-LND(2) or PA83 (List Labs, Campbell, CA) according to the above schedule, …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Reagents:

Anthrax lethal factor (batch 1692A1B), and protective antigen (batch 17117A1B) from List Biological Laboratories.

Discussion:

In mouse macrophages of sensitive strains, LT triggers the formation of the NLRP1b inflammasome, culminating in pyroptosis and in the secretion of mature IL-1 and active caspase-1…

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

List Labs Product #169A (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence; Lyophilized) has been discontinued; however, Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence; Liquid) is available for purchase.

• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence