Citations

Bacterial Toxin Research Citations

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344 citations found

Product: MAPKKide® Peptide Substrate (DABCYL/FITC) for Anthrax Lethal Factor

Cationic quaternized aminocalix[4]arenes: cytotoxicity, haemolytic and antibacterial activities

Ukhatskaya, EV;Kurkov, SV;Hjlmarsdttir, MA;Karginov, VA;Matthews, SE;Rodik, RV;Kalchenko, VI;Loftsson, T;

Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Biophysical characterization and immunization studies of dominant negative inhibitor (DNI), a candidate anthrax toxin subunit vaccine

Iyer, V;Hu, L;Schant, CE;Vance, D;Chadwick, C;Jain, NK;Brey, RN;Joshi, SB;Volkin, DB;Andra, KK;Bann, JG;Mantis, NJ;Middaugh, CR;

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Substrate Cleavage Profiling Suggests a Distinct Function of Bacteroides fragilis Metalloproteinases (Fragilysin and Metalloproteinase II) at the Microbiome-Inflammation-Cancer Interface

Shiryaev, SA;Remacle, AG;Chernov, AV;Golubkov, VS;Motamedchaboki, K;Muranaka, N;Dambacher, CM;Capek, P;Kukreja, M;Kozlov, IA;Perucho, M;Cieplak, P;Strongin, AY;

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

  • Anthrax protective antigen-83 (PA83) was purchased from List Biological Laboratories. …

    MPII Proteolysis of Proteins in Vitro:

    Anthrax PA83 (2 g; 1 m) was co-incubated for 1 h at 37 C with increasing concentrations of MPII in 50 mm HEPES, pH 8.0, containing 1 mm CaCl2, 0.5 mm MgCl2, and 10 m ZnCl2. The total volume of the reactions was 20 l. Where indicated, GM6001 (1 m) was added to the reactions to inhibit MPII. The cleavage reaction was stopped by adding a 5 SDS sample buffer. The digest samples were analyzed by SDS-PAGE using 420% polyacrylamide gel.

Calpain-dependent cytoskeletal rearrangement exploited for anthrax toxin endocytosis

Jeong, SY;Martchenko, M;Cohen, SN;

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

  • Chemicals and Reagents:

    PA and LF were purchased from List Biological Laboratories…Batches of PA and LF had different potency, accounting for the different amounts used in different experiments, each of which included control data for the same toxin batch.

    Toxin Treatment and Cell-Viability Assay:

    Cells were seeded in a 96-well plate at a density of 2 105 cells/mL 1 d before toxin treatment. Various concentrations of PA combined with a fixed concentration of LF (200 ng/mL) were added to the wells, and cells were incubated for 4 h at 37 C.

    Immunofluorescence Microscopy.

    PA protein was labeled with Alexa-Fluor 488 using the protein-labeling kit…

    Biochemical Assay of PA Binding and Internalization.

    Cells were exposed to 1 g/mL of PA at 4 C for 1 h for the binding assay and then were shifted to 37 C for the indicated time for the internalization kinetics assay.

    Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Anthrax lethal toxin induces acute diastolic dysfunction in rats through disruption of the phospholamban signaling network

Golden, HB;Watson, LE;Nizamutdinov, D;Feng, H;Gerilechaogetu, F;Lal, H;Verma, SK;Mukhopadhyay, S;Foster, DM;Dillmann, WH;Dostal, DE;

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Toxin inhibition of antimicrobial factors induced by Bacillus anthracis peptidoglycan in human blood

Barua, S;Iyer, JK;Larabee, JL;Raisley, B;Hughes, MA;Coggeshall, KM;Ballard, JD;

Product: Anthrax Edema Factor (EF), Recombinant from B. anthracis

Enhanced production of antibody fragment via SRP pathway engineering in Escherichia coli

Lee, YJ;Jeong, KJ;

Product: Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Anthrax toxin-induced rupture of artificial lipid bilayer membranes

Nablo, BJ;Panchal, RG;Bavari, S;Nguyen, TL;Gussio, R;Ribot, W;Friedlander, A;Chabot, D;Reiner, JE;Robertson, JW;Balijepalli, A;Halverson, KM;Kasianowicz, JJ;

Product: Anthrax Protective Antigen, Activated (PA 63) from B. anthracis