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December 31, 2013
Thesis
Sardo, JL;
Product: MAPKKide® Peptide Substrate (DABCYL/FITC) for Anthrax Lethal Factor
Screening Against LF:
… Each well contained a volume of 90 L including buffer (20 mM HEPES, pH 7.4), recombinant LF (10 nM final concentration, List Biological Laboratories), and the fragment solution (1 mM final concentration). After a 20 min incubation period at 25 C, the reaction was initiated by the addition of 10 L fluorogenic LF substrate (2 M final concentration, MAPKKide DABCYL/FITC, List Biological Laboratories).
Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence
December 15, 2013
International Journal Of Pharmaceutics
Ukhatskaya, EV;Kurkov, SV;Hjlmarsdttir, MA;Karginov, VA;Matthews, SE;Rodik, RV;Kalchenko, VI;Loftsson, T;
Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis
Cytotoxicity studies: Recombinant B. anthracis lethal factor was purchased from List Biological Laboratories (Campbell, CA, USA). …
Author did not specify which List Labs Lethal Factor was utilized. List Labs offers Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
December 1, 2013
Thesis
Li, X;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
… B. anthracis rPA protein was from List Biological Laboratories, Inc. (Campbell, CA). …
November 1, 2013
Human Vaccines And Immunotherapeutics
Iyer, V;Hu, L;Schant, CE;Vance, D;Chadwick, C;Jain, NK;Brey, RN;Joshi, SB;Volkin, DB;Andra, KK;Bann, JG;Mantis, NJ;Middaugh, CR;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Materials and Methods:
… Recombinant protective antigen (rPA) of recent production from List Biological Laboratories Inc. was used as a control for these studies.
October 21, 2013
Journal Of Biological Chemistry
Shiryaev, SA;Remacle, AG;Chernov, AV;Golubkov, VS;Motamedchaboki, K;Muranaka, N;Dambacher, CM;Capek, P;Kukreja, M;Kozlov, IA;Perucho, M;Cieplak, P;Strongin, AY;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
… Anthrax protective antigen-83 (PA83) was purchased from List Biological Laboratories. …
MPII Proteolysis of Proteins in Vitro:
Anthrax PA83 (2 g; 1 m) was co-incubated for 1 h at 37 C with increasing concentrations of MPII in 50 mm HEPES, pH 8.0, containing 1 mm CaCl2, 0.5 mm MgCl2, and 10 m ZnCl2. The total volume of the reactions was 20 l. Where indicated, GM6001 (1 m) was added to the reactions to inhibit MPII. The cleavage reaction was stopped by adding a 5 SDS sample buffer. The digest samples were analyzed by SDS-PAGE using 420% polyacrylamide gel.
October 15, 2013
PNAS
Jeong, SY;Martchenko, M;Cohen, SN;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Chemicals and Reagents:
PA and LF were purchased from List Biological Laboratories…Batches of PA and LF had different potency, accounting for the different amounts used in different experiments, each of which included control data for the same toxin batch.
Toxin Treatment and Cell-Viability Assay:
Cells were seeded in a 96-well plate at a density of 2 105 cells/mL 1 d before toxin treatment. Various concentrations of PA combined with a fixed concentration of LF (200 ng/mL) were added to the wells, and cells were incubated for 4 h at 37 C.
Immunofluorescence Microscopy.
PA protein was labeled with Alexa-Fluor 488 using the protein-labeling kit…
Biochemical Assay of PA Binding and Internalization.
Cells were exposed to 1 g/mL of PA at 4 C for 1 h for the binding assay and then were shifted to 37 C for the indicated time for the internalization kinetics assay.
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
October 9, 2013
International Journal of Cardiology
Golden, HB;Watson, LE;Nizamutdinov, D;Feng, H;Gerilechaogetu, F;Lal, H;Verma, SK;Mukhopadhyay, S;Foster, DM;Dillmann, WH;Dostal, DE;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
LT treatment of NRVM:
Approximately 24 h after NRVM plating, NRVM were treated with LT in a 2:1 ratio of PA:LF prepared in dispersion medium. PA (activated, 63 kD segment) and LF were purchased from List Biological Laboratories (Campbell, CA) and each reconstituted in glycerol at a concentration of 0.5 mg/mL.
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
October 1, 2013
Infection And Immunity
Barua, S;Iyer, JK;Larabee, JL;Raisley, B;Hughes, MA;Coggeshall, KM;Ballard, JD;
Product: Anthrax Edema Factor (EF), Recombinant from B. anthracis
Human cytokine antibody array assay:
After separation by centrifugation, WBCs were washed twice with complete RPMI 1640 medium, and the cells were resuspended in RPMI 1640 supplemented with 10% fetal bovine serum and penicillin-streptomycin. WBCs (5 106 to 10 106 cells per ml) were treated with ET (25 pmol PA plus 25 pmol EF), LT (25 pmol PA plus 25 pmol LF), …
The following materials were used in this study. B. anthracis EF, LF, and PA were purchased from List Biological Laboratories (Campbell, CA).
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
Author did not specify which List Labs Anthrax Edema Factor was utilized. List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).
August 14, 2013
Biotechnology And Bioprocess Engineering
Lee, YJ;Jeong, KJ;
Product: Anthrax Protective Antigen, Activated (PA 63) from B. anthracis
Enzyme-linked immunosorbent assay (ELISA):
A 50 L of a 5 g/mL solution of activated PA63 (List Biological Labs, Campbell, CA, USA) dissolved in 50 mM carbonate-bicarbonate buffer (pH 9.6) (Sigma-Aldrich) was used to coat each well of a 96-well transparent flat plate …
August 14, 2013
The Journal of Chemical Physics
Nablo, BJ;Panchal, RG;Bavari, S;Nguyen, TL;Gussio, R;Ribot, W;Friedlander, A;Chabot, D;Reiner, JE;Robertson, JW;Balijepalli, A;Halverson, KM;Kasianowicz, JJ;
Product: Anthrax Protective Antigen, Activated (PA 63) from B. anthracis
Reagents:
…Recombinant forms of Bacillus anthracis PA63, LF, and EF were obtained from List Biological Laboratories (Campbell, CA) and reconstituted per the manufacturer’s instructions. …
The following applications were performed using the above listed Anthrax products:
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
Author did not specify which List Labs Anthrax Edema Factor was utilized. List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).
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