Bacterial Toxin Research Citations

Recombinant proteins and reagents:

Protective antigen (PA) and edema factor (EF) were purchased from List Biological. …

Cell seeding and incubation with toxins:  

For toxin treatment, patterned and nonpatterned peritoneal macrophages and BMDMs were incubated with PA at 1 g/ml and EF at 100 ng/ml for 6 or 16 h at 37C in a 5% CO₂ atmosphere. For some experiments, cells were incubated with PA alone at 1 g/ml.

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

… Recombinant protective antigen of anthrax (PA) and recombinant lethal factor (LF) were purchased from List Biologicals (Campbell, CA, USA). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Immunization screening:

The threshold dose of PA for rabbits was chosen by injecting rabbits intramuscularly (i.m.) on days 0 and 14 with either 10 g, 1 g, 0.1 g, 0.01 g, or 0 g of PA (List Biological Laboratories, Campbell, Calif.) diluted in PBS in a volume of 0.5 ml, each adsorbed to 1 mg of aluminum per ml … The primary and secondary antibody response profiles were examined by injecting rabbits i.m. with 50 g of PA/Alhydrogel on days 0 and 21 and collecting blood periodically to test serum for the presence of antibodies to PA and TNA titers in the serum.

2.3. Protection against aerosol exposure

Rabbits were injected i.m. with 0.1 g of PA/Alhydrogel and boosted with 0.1 g of PA/Alhydrogel 4 days (day 86), 8 days (day 82), or 12 days (day 78) before aerosol exposure on day 90 following the primary immunization. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

PA and LF were obtained from List Biological Laboratories, Inc. ,C ampbell, USA. The cells were incubated at 37 8 Ca nd 5% CO 2 for 3h. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

…Recombinant protective antigen (PA) of anthrax and recombinant lethal factor (LF) were purchased from List Biologicals (Campbell, CA, USA). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Protective Antigen (PA) was from List Biological Laboratories, Campbell, CA, USA. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Proteins and peptides:

Immobilized PA83 (LIST Biological Laboratories, Inc.) was used in the ELISA to establish titers of PA-reactive Ab in the clinical samples. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Mice were injected in both thigh muscles with 50 L of a formulation containing…or 20 g/mL anthrax recombinant protective antigen (rPA) (List Biological Laboratories, Campbell, CA)…

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Reagents:

…LF, EF, PA83, and PA63 were obtained from List Biological Laboratories (Campbell, CA). …

Animal care and study protocol:

…Total LF and LTx analysis was determined in two animals, 16N and 89N, over the course of infection. …

Antibody specificity:

Specificity of anti-PA and anti-LF MBs was demonstrated for LTx, LF, and PA. PA63/LF were mixed at a w/w ratio of 10:1 to form LTx complex. PA83 and LF were mixed as a non-complexed control. Mixtures were incubated at room temperature (RT) for 30 min. LF, PA63, PA83, LF/PA63, and LF/PA83 at 0.5 g LF and 5 g PA83 or PA63 were immunoprecipitated with anti-LF and anti-PA MBs, …

Lethal toxin standard preparation and characterization:

LTx was formed by mixing PA63 and LF at w/w ratios of 0:1 to 60:1 PA63/LF, …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

Mouse anti-CFA and anti-toxin antibody titration:

Anti-CFA/I and anti-CS1, -CS2, -CS3, -CS4/CS6, -CS5/CS6, and anti-STa and anti-LT IgG antibodies in serum of each mouse were titrated as described previously [34,3739]. Briefly, 500 ng CFA/I, CS1, CS2, CS3, CS4/CS6, and CS5/CS6 heat-extracted from ETEC field isolates or recombinant E. coli strains [39] (Table 1), or 100 ng LT (List Biological Laboratories, Inc., Campbell, CA) were coated to each well of H2B plates …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis