Citations

Induction and clinical scoring of EAE:

…Bordetella pertussis toxin (300 ng; List Biological Laboratories, Inc.) was diluted in 0.2 ml normal saline and injected intraperitoneally immediately after PLP or MOG immunization and two days after MOG immunization in C57Bl/6 mice. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… . pertussis toxin 200ng(List Biological laboratories, USA) 2 . , . EAE …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and clinical evaluation:

An i.v. injection of 200 ng pertussis toxin (PTX; List Biological Laboratories, Campbell, CA) was also administered on days 0 and 2 of the immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

… with 200 µg MOG35-55 peptide…on day 0. After immunization, 200 ng Pertussis Toxin (List Biological Laboratories, Inc., Campbell …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Retrograde labeling of CeA-RVLM neurons:

…0.5% solution of cholera toxin B subunit (CTB; List Biologicals, Campbell, CA) dissolved in phosphate-buffered saline (PBS) were microinjected in a volume of 50 nl. The micropipette was left undisturbed for 10 minutes to avoid diffusion of tracer along the pipette track. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Histology and Immunohistochemistry

… For mapping of Cholera Toxin Subunit B-injected brains, at the time of ICH induction, 0.5 μL of retrograde neuronal tracer Cholera Toxin Subunit B (CTb, List Biological Laboratories, Campbell, CA, USA, diluted to 20 μg/μL in 10% DMSO) was infused with collagenase, blood, or alone. Seven days after treatment, animals were killed, and brains were fixed, frozen, and coronally sectioned at 50 μm. A series of six sections spaced 200 μm apart were blocked in 5% normal donkey serum and 0.3% Triton, incubated with a CTb primary antibody (Goat anti-CTb, 1:500, List Biological Laboratories) overnight,

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Experimental Autoimmune Encephalomyelitis:

EAE immunization was performed as previously described … Mice received 200 ng of pertussis toxin (List Biologicals) intraperitoneally on day 0 and 1 after immunization. Two investigators, blinded to the genotype of mice, independently analyzed the mice daily on a 5-point scale: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalitis induction and treatment protocols:

… Mice also received 100 µl of 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) by intraperitoneal injection concomitantly and again 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Study animals:

Thirty-four healthy Quarter Horse foals and their respective dams were used for this study. …Individual foals were randomly assigned to the following groups: 1) EBRE 1 group (n=9) which received 210¹⁰ CFUs of R. equi inactivated by 4 kGy of eBeam radiation in 100 ml of saline adjuvanted with 100 µg of cholera toxin B subunit (CTB, List Biological Laboratories, Campbell, CA, USA); …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cell culture studies:

…Mouse peritoneal macrophages where isolated from C57BL/6J, C3H/HeJ and CD44 ^-/- four days after an IP injection of thioglycolate as previously described [36]. …Cells were grown in DMEM…overnight, rinsed with PBS and then adherent cells treated in 1% FBS alone as a control or stimulated with LPS (List Biological Inc., Campbell, CA, #201). Cells were treated for 4 hours except for time-course experiments. …

• Product #201 – LPS from Escherichia coli O111:B4