Bacterial Toxin Research Citations

STEC cultures and Shiga toxins (Stx): Purified Stx1 and Stx2a were purchased from List Biological Laboratories (Campbell, CA, USA). …

• Product #161 – Shiga Toxin 1 from Escherichia coli
• Product #162 – Shiga Toxin 2 from Escherichia coli

… Purified CT (Cat. No. 100B) and diphtheria toxin (Cat. No. 150) were purchased from List Biological Laboratories (Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Ganglioside GM1 (GM1) was detected using 1 µg/ml biotinylated cholera toxin B subunit (List Biological Laboratories Inc., Campbell, CA) …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

Tracer injections:

Rats (6 to 7 weeks old, 260 to 300 g, Charles River) were anesthetized as described above and placed in a stereotaxic apparatus. Glass micropipettes (4 to 5 m in outer diameter) were backfilled with a 1% solution of CTb [List Biological Laboratories; diluted in 0.1 M phosphate buffer (PB) at pH 6.0] for injection in the dentate gyrus (n = 4), the retrosplenial cortex (n = 4), and the SUM (n = 4). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

…Thus, this study used anterograde tracer biotinylated dextran amine and the retrograde tracer cholera toxin B subunit to examine the organization of the thalamocortical projections of the AV.

… A glass micropipette (2030 m id) filled with 1% low salt CTB (List Biological Laboratories, Campbell, CA) in distilled water was stereotaxically lowered into part of the left retrosplenial cortex or presubiculum where dense terminal labeling had been present in the anterograde …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Neural tracing experiments

Five wild type (WT) mice and five cPcdh-a KO mice of aged 8–10 weeks were anesthetized intraperitoneally with a mixture ofketamine (300 mg/kg; Daiichi-Sankyo, Tokyo, Japan) and xylazine(30 mg/mL; Bayer, Leverkusen, Germany). The cholera toxin bsubunit (CTb, 5 ng in 1 µL of saline; List Biological Laboratories,Campbell, CA, USA), an anterograde neural tracer, was injectedinto the left eye using a glass pipette. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… de cada grupo foram submetidos a uma injeo intraocular de uma soluo aquosa do traador neuronal antergrado CTb (List Biological Laboratories, Inc., Campbell, CA) a 0,1% e dimetilsufxido a 10%. Os animais foram anestesiados, por via intramuscular, com quetamina …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Anterograde labelling of retinal afferents:

To identify the retinofugal projection, four days before sacrifice, 2.5 µl of the orthogradely transported tracer cholera toxin subunit beta (CTB) were intravitreally injected (1%, diluted in distilled water, List Biological Laboratories, Campbell, CA, USA) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Mouse anti-CFA and anti-toxin antibody titration:

Anti-CFA/I and anti-CS1, -CS2, -CS3, -CS4/CS6, -CS5/CS6, and anti-STa and anti-LT IgG antibodies in serum of each mouse were titrated as described previously [34,3739]. Briefly, 500 ng CFA/I, CS1, CS2, CS3, CS4/CS6, and CS5/CS6 heat-extracted from ETEC field isolates or recombinant E. coli strains [39] (Table 1), or 100 ng LT (List Biological Laboratories, Inc., Campbell, CA) were coated to each well of H2B plates …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Overview of experiments:

Two experiments were conducted to assess the role of the connection between the VP-m and MD and the VP-m and VTA during specific PIT. Experiment 1 aimed to investigate the neuronal activity during specific PIT of neurons in the VP-m projecting to the MD or the VTA using the neuronal activity marker c-Fos combined with retrograde tracers, Cholera toxin-b subunit (CTb, catalog #104; List Biological Laboratories)…

Histology – CTb and FG injection sites:

To locate the injection sites, sections containing the MD were stained using a goat anti-CTb antibody for visualization of the injection site…After, sections were incubated for 48 h in 4°C in a solution containing the anti-CTb antibody (1:2000, catalog #703; List Biological Laboratories),…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)