Bacterial Toxin Research Citations

Preparation of murine cells:

…PB cells were collected by cardiac puncture of anesthetized mice and monocytes were isolated as indicated for BM monocytes. Bronchoalveolar lavage fluid was collected by instilling 2.0mL of normal saline via a transtrancheal incision in anesthetized mice that had received an intratracheal injection of 5 g of lipopolysaccharide (from E. coli J5 (RC), List Biological Laboratories, Campbell, CA) or normal saline 24h earlier. Mouse peritoneal macrophages were harvested from the peritoneal cavity of wild-type, NCAM/ and NRP-2/ mice 3 days after the intraperitoneal injection of 3% thioglycollate and were placed in culture for 24 h…

• Product #301 – LPS from Escherichia coli J5 (Rc)

Preparation and Use of Primary Microglial Cultures:

100 ng/mL Escherichia coli LPS (List Biological Laboratories, Campbell, CA) as a TLR4 stimulus, …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Pharmacologic agents:

… Pertussis toxin (PTX; List Biological laboratories, Inc., CA) was dissolved in PBS and added to cells at a working concentration of 100 ng/ml 30 min before the addition of cPA or LPA. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and Scoring of EAE:

Experimental autoimmune encephalomyelitis was induced as in prior studies. …In addition, each animal received 200 ng pertussis toxin (List Biological, Campbell, CA, USA) in 0.1 mL PBS by intraperitoneal (IP) injection at 0 and 48 hours post immunization. Severity of EAE was scored…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and clinical scoring of EAE:

…Bordetella pertussis toxin (300 ng; List Biological Laboratories, Inc.) was diluted in 0.2 ml normal saline and injected intraperitoneally immediately after PLP or MOG immunization and two days after MOG immunization in C57Bl/6 mice. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… . pertussis toxin 200ng(List Biological laboratories, USA) 2 . , . EAE …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and clinical evaluation:

An i.v. injection of 200 ng pertussis toxin (PTX; List Biological Laboratories, Campbell, CA) was also administered on days 0 and 2 of the immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

… with 200 µg MOG35-55 peptide…on day 0. After immunization, 200 ng Pertussis Toxin (List Biological Laboratories, Inc., Campbell …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Retrograde labeling of CeA-RVLM neurons:

…0.5% solution of cholera toxin B subunit (CTB; List Biologicals, Campbell, CA) dissolved in phosphate-buffered saline (PBS) were microinjected in a volume of 50 nl. The micropipette was left undisturbed for 10 minutes to avoid diffusion of tracer along the pipette track. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Histology and Immunohistochemistry

… For mapping of Cholera Toxin Subunit B-injected brains, at the time of ICH induction, 0.5 μL of retrograde neuronal tracer Cholera Toxin Subunit B (CTb, List Biological Laboratories, Campbell, CA, USA, diluted to 20 μg/μL in 10% DMSO) was infused with collagenase, blood, or alone. Seven days after treatment, animals were killed, and brains were fixed, frozen, and coronally sectioned at 50 μm. A series of six sections spaced 200 μm apart were blocked in 5% normal donkey serum and 0.3% Triton, incubated with a CTb primary antibody (Goat anti-CTb, 1:500, List Biological Laboratories) overnight,

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)