Bacterial Toxin Research Citations

… In most cases, we added 1% cholera toxin b subunit (CTb, Lot #104, List Biological Laboratories, USA) to …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Cholera toxin was purchased from List Biologicals Laboratories, Inc. (Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Experimental protocol:

Each sheep was placed supine in the PET scanner with the caudal end of the field of view just superior to the dome of the diaphragm. …After the initial set of scans, all sheep received a continuous infusion of endotoxin (Escherichia coli O55:B5, 10 ng/kg/min intravenously; List Biological Laboratories Inc, Campbell, CA, USA).

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

EAE induction:

On day 0 and 2 post-immunization, i.p. injection of 400 or 300 ng of Bordetella pertussis toxin (PT; List Biological Laboratories, Campbell, CA) was respectively performed for optimal or suboptimal EAE

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… and PA was obtained from List Biological Laboratories (Campbell, CA, USA). …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Allergic Sensitization and Anaphylaxis:

Mast cells (mice) were depleted by i.p. injection of diphtheria toxin (List Biological Laboratories) in saline over 3 days: 100 ng day 1, 500 ng day 2, and 500 ng day 3.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents:

For the bilayer lipid measurements, PA 63 was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA).

Channel Reconstitution into Planar Lipid Bilayers:

…Single channels were formed by adding 0.5 to 1 L of 20g mL 1 solution of PA63, or 0.2 to 0.5 L of 48 ng mL 1 solution of C2IIa to the 1.5 mL aqueous phase on the cis-half of the bilayer chamber. For multichannel experiments, we applied 12 L of 0.2 mg mL1 stock PA63 or 12 L of 48g mL 1 stock C2IIa to the cis-side of the membrane. Under this protocol, PA63 and C2IIa channel insertions were always
directional as judged by channel conductance asymmetry in the applied transmembrane voltage…In most of the experiments, the PAMAM dendrimers were added to the cis-compartment of a bilayer chamber, which was the side of PA63 and C2IIa addition.

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

 Immunization protocols and collection of serum samples:

Cholera antigens (Table 2) were … CT (List Biological Laboratories, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… 4. Pertussis toxin (PT) from Bordetella pertussis, lyophilized in buffer (List Biological Laboratories, Inc.). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 7. Pertussis Toxin, if required (see Table 1; List Biologicals). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)