Citations

Cell culture and treatment:

Experiments were performed with a human pulmonary mucoepidermoid carcinoma cell line NCI-H292 (ATCC), which was maintained in RPMI 1640 medium containing 10% fetal bovine serum and 1% penicillin/streptomycin at 37°C and 5% CO2. … Additional studies were performed with primary mouse tracheal epithelial (MTE) cells, which were isolated from the tracheas of C57BL/6 J mice (Jackson Laboratories, Bar Harbor, ME) using overnight incubation with 0.1% protease 14 (Sigma-Aldrich, St. Louis, MO) and cultured on rat tail collagen I gel (BD Biosciences, San Jose, CA) in DMEM/F12 media (Invitrogen, Grand Island, NY) supplemented with 20 ng/mL cholera toxin (List Biological Laboratories, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Topical Skin Immunization:

…After evaporation of the acetone, 25 l of an aqueous mixture containing 1 mg/ml cholera toxin (CT) adjuvant (List Biological Labs, Campbell, CA) was applied to each ear and uniformly spread with a small paint brush. Control mice received only the CT adjuvant, while experimental mice also received 50 l of an aqueous mixture containing 100 mg/ml ovalbumin (OVA) Ag (Sigma-Aldrich product #A5503) on each ear.

Treatment of Lang-DTR mice with Diphtheria Toxin:

For some cocultures (as indicated), WT or Lang-DTR mice were treated with diphtheria toxin (DT) (List Biological Labs, Campbell, CA). Mice were injected intraperitoneally with 1 µg DT in 100 µl PBS. Mice were treated one day preceding immunization and one day after…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Induction of EAE:

EAE was induced with an immunization protocol utilizing MOG 33-55 (AnaSpec Inc., Fremont, CA, USA), which leads to T cell infiltration in the central nervous system, in combination with pertussis toxin (List Biological Laboratories, Campbell, CA, USA), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

… Mice were also injected intraperitoneally with 200 ng pertussis toxin (List Laboratories, Campbell CA, USA) in phosphate buffered saline (PBS) on D0 and D2 following MOG immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antibodies, cytokines and inhibitors:

…Lipopolysaccharide (LPS, Escherichia coli 0111:B4) was from List Biological Laboratories, was used at 100 ng/ml.

• Product #201 – LPS from Escherichia coli O111:B4

Anterograde tracing of corneal afferent fibers:

Cholera toxin B subunit (CTb; 1% solution in 0.1 M phosphate buffer; List Biological Laboratories, Inc., Campbell, CA) was used as an anterograde tracer to identify central projections of corneal afferent fibers to the trigeminal dorsal horn (Hegarty et al. 2010;Todd et al. 2003;Aicher et al. 2013). Following FG injections, several drops of ophthalmic proparacaine hydrochloride, 5% solution (Bausch and Lomb, Tampa, FL) were placed in the left eye to reduce spontaneous eye movements and a 1% CTb solution (8 12 l) was placed on the corneal surface inside a steel ring for 30 min as previously described (Hegarty et al. 2010). Rats received a subcutaneous injection of ketoprofen (2.5 mg/kg) to reduce discomfort and were monitored during recovery from anesthesia.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Neonatal cardiomyocytes and fibroblasts:

… Cardiac cells were starved in serum-free medium (plating medium without serum) 24 hours before treatment with 10 ng/ml IL-1 (501-RL-010, R&D Systems, Minneapolis, MN) and 50 ng/ml TNF- (PMC3014, BioSource International, Camarillo, CA) separately and combined, and 1000 ng/ml lipopolysaccharide (LPS) (List laboratories, Campbell, CA) for 24 h. Cell medium was ultrafiltrated using Amicon Ultra-4 10 kDa centrifugal filter (Millipore, MA) before protein analyses.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Induction of EAE:

… Pertussis toxin (List Biological Laboratories, CA) was intraperitoneally administered at a dose of 400ng on post immunization days 0 and 2. A booster injection was given on day 7 after the initial immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and evaluation of EAE:

Female mice, 6 to 8 weeks of age, were immunized subcutaneously in the flanks with 300 µg MOG³⁵⁵⁵ in phosphate buffered saline (PBS) emulsified in an equal volume of complete Freund’s adjuvant (CFA, Sigma-Aldrich, St. Louis, MO) supplemented with 1 mg Mycobacterium tuberculosis H37RA (Difco, Detroit, Michigan). Immediately afterwards, and again 48 hours later, mice received i.p. injections of 300 ng pertussis toxin (List Biological Labs, Campbell, CA).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Tetanus toxoid List Biologicals Campbell, USA …

• Product #191 – Tetanus Toxoid from Clostridium tetani