Bacterial Toxin Research Citations

Induction of EAM and DCMi:

On day 0, mice also received 500 ng pertussis toxin i.p. (List Biologicals).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

8-wk-old female mice were immunized by s.c. injection at the base of the tail…The mice also received i.v. injections of 200 ng pertussis toxin (List Biological Laboratories) on days 0 and 2 and were administered i.p. 100 µg anti4-1BB (clone 3H3) or control antibody (rat IgG) on days 0, 2, 4, and 8.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

EAE was induced as described [19]. Female C57BL/6 mice were subcutaneously injected with 100 µg of MOG3555 (myelin oligodendrocyte glycoprotein; AnaSpec) in IFA (incomplete Freund’s adjuvant; Difco) containing 250 µg of Mycobacterium tuberculosis (Difco). On the same day and again 48 hours later, mice received an intraperitoneal injection of 200 ng of pertussis toxin (List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mouse model of autoimmune encephalomyelitis:

Experimental autoimmune encephalomyelitis was induced as previously described (Waiczies et al., 2012). In brief, 6-8 week old female SJL/J mice (Janvier, France) were subcutaneous immunised with 250 mg PLP139151 (Pepceuticals Ltd., UK; purity 95%) along with Complete Freunds Adjuvant and heat-inactivated Mycobacterium tuberculosis (H37Ra, Difco,, USA). Bordetella pertussis toxin (250 ng; List Biological Laboratories, USA) was administered intraperitoneally on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Mice were also injected intraperitoneally with 200 ng of pertussis toxin (PTX; List Biological Laboratories, Campbell, CA, USA) on the day of immunization. Evaluating clinical EAE. All the mice were …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Macrophage Polarization and Supernatant Cytokine Assay:

BMDM were seeded in 6-well plates at 210⁶ cells/well and polarized for 48 hours with the addition of 50 ng/ml LPS (List Biological Labs) and 50 ng/ml IFN (Cedarlane) for M1, or 40 ng/ml IL-4 (R&D Systems) for M2 at 37C in a 5% CO₂ humidified atmosphere. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… Pertussis toxin List Biological Laboratories …

Myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmunity in C57Bl/6 mice:

Immunization – 

3.  Fill a 1 ml insulin syringe with 100 µl of Pertussis toxin (5 µg/ml) avoiding making bubbles. Slowly inject the solution in the tail vein. Remove the needle and press for a few seconds with a piece of paper to plug the bleeding; …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Enzyme-linked immunosorbent assay (ELISA): 

An ELISA was used to measure the levels of IgG against the toxins and bacterin. Blood samples were collected before (pre-immune) immunization as well as 11 and 17 weeks after the first immunization. Purified TcdA (List Biological Laboratories, USA), …

• Product #152C – Toxin A from Clostridium difficile

Reagents:

LPS (cat. no. 421),…were purchased from List Biological Laboratories (Campbell, CA),…

Generation of stable control (nontargeting) and MyD88 and TLR2 knockdown RAW 264.7 cells:

…Functional knockdown was assessed by using known agonists (LPS and Pam3CSK4) followed by measurement of COX-2 protein induction and/or agonist-mediated cytokine secretion. Two cell pools with the greatest degree of knockdown as well as the NT cell pool were used in the acylcarnitine treatment experiments.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Experimental autoimmune encephalomyelitis:

The procedure to induce EAE was performed according to the instructions. …Pertussis toxin (200 ng per mouse; List Biological Laboratories) in PBS was administered i.v. on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)