Bacterial Toxin Research Citations

… Pertussis toxin List Biological Labs Cat #179 A …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of experimental autoimmune orchitis (EAO)

Age-matched male Gsdmdfl/flCx3cr1-cre and Cx3cr1-cre mice were used to induce EAO, as described previously (Nicolas et al, 2017). First, the testicular homogenate (TH) was obtained from decapsulated testes of adult syngeneic mice and homogenized in an equal volume of sterile PBS. Second, TH was mixed with incomplete Freund’s adjuvant (IFA; Sigma-Aldrich, F5506) containing 1 mg/ml Mycobacterium tuberculosis (BD, 231141) in 1:1 ratio. Finally, the mice were immunized thrice every 14 days with the mixture, followed by an intraperitoneal injection of 100 ng pertussis toxin (List Biological Laboratories, lot#180) in 100 μl PBS. Each animal was immunized dorsally at four sites near the popliteal lymph nodes in a total volume of 200 μl. The testes were collected for further analysis 50 days after the first immunization.

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… of heat-killed Mycobacterium tuberculosis H37RA. This procedure was performed on day 0. Additionally, each mouse received 200 ng of pertussis toxin from List Biological Laboratories …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Additionally, animals received pertussis toxin (List Biological Laboratories, Campbell, CA), intraperitoneally (100 ng/mouse), on days 0 and 2 after the first immunization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

3.5. Stereotaxic surgery

… Under visual control using asurgical microscope, the glass capillary used for injection was moved 750 µm lateral to the
posterior median sulcus in the midlevel of the space between the superior rim of the posterior arch of atlas and the inferior border of the occipital bone. Here, the tip of the capillary was 30 perpendicularly introduced 100 µm deep into the laminae II-III of the STN. 2×20 nL of CTB (Cat. No.: #104, List Biological Laboratories) was injected in two steps with an automated injector. After 2 min, the needle was slowly removed. Finally, the muscles and the skin were sutured. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Cholera Toxin B (CTB) Purified choleragenoid, the B subunit of cholera toxin List Biological, goat polyclonal, cat. #703, lot #7032A9, …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Bordetella pertussis toxin (List Biological Laboratories, Inc. #181) the day of immunization and again 2 days later. Motor scores reflecting progressive ascending paralysis were assessed daily using the following scoring system: …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… The peptide emulsion was injected subcutaneously at 200 µg per mouse. Pertussis toxin (PTX; List Biological Labs, Campbell, CA, USA) was prepared in PBS and intraperitoneally injected at 400 ng per mouse, at 2 hr and 24 hr post administration of the CFA emulsion. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera toxin List Biological Laboratories (Campbell, CA, USA) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… ULTRA PURE LPS from Escherichia coli O111:B4 List Biological Laboratories 421 …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4