Citations

… Goat anti-PA polyclonal antibody and recombinant PA were obtained from List Labs. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Cell stimulation

… Bacillus anthracis Lethal Factor (0.5 mg mL -1) and Protective Antigen (0.5 mg mL -1) (Lethal toxin, List Labs, …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

… Cholera toxin subunit beta (CTB, 1% in 2 µl PBS; 103B, List Biological) axonal tracer was injected intravitreally 1 day prior to sacrifice. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Cholera toxin List Biologicals Cat#100B …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Experimental autoimmune encephalomyelitis

For induced EAE, mice were immunized subcutaneously with 100 μg of myelin oligodendrocyte glycoprotein (MOG35–55) peptide emulsified in CFA, which was made from incomplete Freund adjuvant (Difco) plus Mycobacterium tuberculosis (Difco). Mice were intraperitoneally injected with 200 ng of pertussis toxin (List Biological Laboratories) on the day of immunization and 2 days later. The mice were observed daily for clinical signs and scored as described previously. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Intracellular cytokine staining

… Cells were plated at a density of 1 × 106 cells/well in a 96-well U-bottom plate and stimulated at 37°C with either 1 μg/ml of each peptide in the pool, 0.25 μg/ml staphylococcal enterotoxin type B (List Biological Laboratories), or 0.2% DMSO (Sigma-Aldrich). After 6 h, stimulations were stopped by adding EDTA at a final concentration of 2 mM, and samples were stored overnight at 4°C.

Antibodies and reagents

… PTX was from List Biological Laboratories. …

Calcium fluorimetry

… For studies of PTX inhibition of calcium signaling, cells were treated with 100 ng/ml PTX or vehicle concurrent with addition of doxycycline. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Additionally, they received 150ng PT (List laboratories) on day 0 and 2 after immunization. To induce EAE in RAG1 KO mice, CD4+ T cells were isolated from …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… All animals were injected with 250 ng pertussis toxin (List Biological Laboratories, PTX181, USA) intraperitoneally on the day of immunization and on day 2 after first …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Neuronal ablation Mice carrying the Hes2iCre;hCdx2::FlpO alleles in addition to the effector Tauds-DTR received i.p. injections of diphtheria toxin (DTX, 50 ng/gram of weight; List Biological Laboratories) at P28 and P31, or saline solution in equal volume as control. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae