Bacterial Toxin Research Citations

… For cell ablation, 1 mg/ml stock solution of DTX (List Biological Laboratories, 150) was prepared with water, aliquoted and stored at -20°C until use. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Immunization was carried out at 0, 7, 14, 21 and 28 days. In the EAM-PTX (+) group, PTX (List Labs, cat. #: 181, Campbell, California, USA) 2 µg was intraperitoneally injected on days 0 and 7. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… C. difficile toxin A (TcdA; List Biological Laboratories, Campbell, CA, USA). …

• Product #152C – Toxin A from Clostridium difficile

Pertussis toxin (PTx) List Biological Laboratories LBL-0901-80

In vivo experimental autoimmune uveitis (EAU) model

C57BL6/J mice were immunized via s.c. injection with 200 μl of emulsion containing 200 μg of IRBP1–20 in Complete Freund’s Adjuvant (CFA), and 0.2 μg of pertussis toxin was added i.p. on the same day. 100K-EVs were administrated via i.v. injection on days 0, 1, 2, and 7 and mice were sacrificed at day 18. After whole eyes were removed and fixed in 4% paraformaldehyde (PFA), sections were cut through the pupillary-optic nerve plane, and H&E staining was performed. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Human Donor SMILE Lenticule Acquisition and Preparation

Fresh lenticules (left and right-eye; n = 113) were obtained from consenting patients (both female and male; age range: 21–50 years) that elected to undergo SMILE laser refractive surgery at the Envision Eye Centre (Sydney, Australia). Lenticules were collected and kept in sterile saline at 4 °C until experimentation. They were conditioned to receive CLECs by submerging them in dKSFM (Thermo Fisher, Waltham, MA) comprising 10 ng mL−1 epidermal growth factor (R&D Systems, Minneapolis, MN), 100 ng mL−1 cholera toxin (List Biological Laboratories, Campbell, CA), and 100 U mL−1 penicillin–streptomycin (Thermo Fisher) for 2 weeks at 37 °C in 5% CO2, otherwise referred to as complete media. Lenticules were used either immediately after conditioning or following gamma (ɣ)-irradiation with 1000cGy for 20 min. Next, they were washed twice in PBS and further incubated in complete dKSFM media without FBS for 3 days prior to receiving a suspension of cells or tissue explants for subsequent cultivation. This strategy was employed to mitotically inactivate and/or deplete viable lenticule-associated keratocytes.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials and Methods

Animals.
Mice of both sexes were used between 4-17 weeks of age and maintained on a C57BL/6
background under specific pathogen-free (SPF) conditions in Allentown caging with aspen shavings and
LabDiet5010 chow (Purina, St. Louis, MO) and RO water. TNFDARE/+ mice8 were rederived by the Genome
Editing Shared Resource, Rutgers Cancer Institute of New Jersey and subsequently crossed to TcrdGDL
mice67. TNF+/+ littermates were used as controls. For suppression assays, wildtype C57BL/6 mice or
TcrdEGFP68 mice were used between 8-14 weeks of age. To assess IEL proliferation, 200 µg of
5-ethynyl2′-deoxyuridine (EdU)(Sigma-Aldrich) was administered i.p. daily for 4 days. To induce gd T cell depletion
in 4-week-old TcrdGDL; TNFDARE/+ mice, 15 ng of diphtheria toxin (List Biological, Campbell, CA) or
endotoxin-free PBS per gram body weight was administered i.p. 24 and 48 h prior to the experiment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Cholera toxin (AZIDE-FREE) from Vibrio cholerae List Biological Laboratories 100B …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE Induction

Female C57BL/6J mice at 6–8 weeks old were purchased from Experimental Animal Center of Air Force Military Medical University. EAE was induced with 200 ug MOG35 − 55 peptide (NJPeptide, China) emulsified in incomplete Freund’s adjuvant (Sigma – Aldrich, USA) added with 5 mg/mL Mycobacterium Tuberculosis H37RA (Difco, USA). Each mouse was immunized with the emulsion subcutaneously into four sites at the back followed by intraperitoneal injection of 200 ng pertussis toxin (List Biological Laboratory). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…A significant part of CRM197 in the world is produced by cultivation of the non-toxic strain C. diphtheriae C7(β197) [11]. This method is used by List Biological Labs (USA). Advantages of this method include correct folding of the target protein and its secretion into the culture medium, which facilitates further purification of the product. …

• Product #149A – Diphtheria Toxin CRM197, Mutant

Experimental Autoimmune Uveitis Model Establishment

We prepared an emulsion by adding Mycobacterium tuberculosis toxin (Difco Laboratories, Franklin Lakes, NJ, USA) to complete Freund’s adjuvant (Difco Laboratories) to achieve a concentration of 2.5 mg/mL, which was then mixed with an equal volume of interphotoreceptor retinoid-binding protein (1–20) (IRBP1–20; 2 mg/mL; GiL Biochem, Shanghai, China) PBS solution. Subsequently, 200 µL of the emulsion was subcutaneously injected into anesthetized mice for immunization. In addition, a 0.25-µg intraperitoneal injection of pertussis toxin (List Biological Laboratories, Campbell, CA, USA) was administered on the day of immunization and the following day. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)