Bacterial Toxin Research Citations

… The dorsal brainstem was exposed via blunt dissection and, following removal of the meningeal membrane, the vagal trigone was exposed. Then, five microinjections of Cholera Toxin B (CTB; List Labs, Campbell, CA, 0.5% in dis+lled water; 60nl per injec+on) were made using a glass pipette attached to a Picospritzer (Toohey Co., Fairfield NJ) into the rostrocaudal extent of the DMV at co-ordinates (in mm) rostro-caudal 0.0-0.6mm from calamus scriptorius, medio-lateral +0.3-0.4 from the midline; dorso-ventral -0.6-0.65 from the surface of the brainstem. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… 2 post-immunisation, mice received an intraperitoneal injection of 200 ng pertussis toxin in 200 µL PBS (List Biological Laboratories, #179A). The mice were monitored and scored daily for EAE symptoms …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… All reactions, including controls without the inhibitors, contained 0.05% DMSO. After 30 minutes of incubation at 37°C under normal cell culturing conditions, pertussis holotoxin (List Biological Laboratories Inc., 179A) was added to the cells at concentrations of 10 or 100 ng/ml. Cells were then incubated for an additional 2 hours, placed on ice, …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Labelling of Lipid A. One mg of commercially obtained lipid A (List Laboratories, Campbell, CA) …

• Product #401 – LIPID A monophosphoryl from Salmonella minnesota R595

… 401 from List Labs). LPS was directly diluted and aliquoted to 100 µg/ml stock solutions in UP water. …

• Product #401 – LIPID A monophosphoryl from Salmonella minnesota R595

… Pertussis toxin was administered on days 0 and 2 (100 ng/mouse, i.p) (List Biological Laboratories, Campbell, CA, USA). Sera were collected on day 0 and day 21 post-immunization at termination …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.4.4 Purification of PA and LF

PA83 was expressed and purified by the Barth group. After purification, PA83 was activated in vitro to PA63 and stored at -80 °C.
LF was purchased from List Biological Laboratories (see 2.1.7) and stored at -80 °C.

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

2.1.2 Study II: Examination of the contribution of neuroinflammatory processes such as
BBB leakage and focal inflammation to changes in mechanical properties

For in vivo MRE and MRI experiments adoptive transfer EAE was induced in 21 SJL mice (female, 9-12 weeks, Janvier, SAS, France, registration number G106/19) by the transfer of myelin proteolipid protein (PLP)-reactive lymphocytes. SJL mice were used as the animal strain of choice to induce EAE (active and adoptive transfer), as they display a high disease susceptibility after induction. PLP-reactive lymphocytes were obtained from 22 donor mice, which were actively immunized as previously described (31, 34) using 200 mg of the myelin peptide PLP139-151 emulsified with 200 ml complete Freund´s adjuvant (Thermo Fischer Scientific, United States) and 800 mg Mycobacterium tuberculosis
H37Ra (Difico, United States). As described in (31), on days 0 and 2 postimmunization (p.i.), 250 ng pertussis toxin (List Biological Laboratories, United States) was injected intraperitoneally. Donor animals were sacrificed on day 10 p.i., axillary and inguinal lymph nodes were collected and cells cultured as described (35). Cells were harvested after 4 days in culture with RPMI 1640 medium (supplemented with 2 mM L-glutamine, 100 units/ml penicillin, 100 mg/ml streptomycin and 10 % fetal bovine serum) (Gibco, Thermo Fischer Scientific) containing 12.5 mg/ml PLP. Into each of the 21 recipient mice 30 million cells were intraperitoneally injected. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.2.1 Active EAE

For Studies I, II and III active EAE was induced in SJL mice. Therefore, 9 – 12-week-old mice (Janvier, SAS, France) were subcutaneously immunized with an emulsion containing 250 µg of a PLP139-151, 800 µg M. tuberculosis H37Ra (Difco, USA) and 100 µl of complete Freund’s adjuvant (CFA; Thermo Fisher Scientific, USA) in addition to an intraperitoneal injection of 250 ng pertussis toxin (List Biological Laboratories, USA) on the same day and 2 days later. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… PTX was from List Biological Laboratories, Inc. (Campbell, CA); …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)