Bacterial Toxin Research Citations

… Pertussis toxin (List Biologicals) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

4.2. Induction and Evaluation of EAE

We induced EAE by subcutaneous (s.c.) sensitization with 100 nmol/mouse of modified PLP139–151 peptide (VSLGKWLGHPDKF, United BioSystems, Herndon, VA, USA) in SJL/J mice, MOG92–106 peptide (DEGGYTCFFRDHSYQ, United BioSystems) in SJL/J mice or A.SW mice, or MOG35–55 (MEVGWYRSPFSRVVHLYRNGK, United BioSystems) peptide in C57BL/6 mice, in which myelin peptides were emulsified in CFA composed of incomplete Freund’s adjuvant (BD, Franklin Lakes, NJ, USA] and Mycobacterium tuberculosis H37 Ra (BD)…. To induce chronic MOG35–55-induced EAE, we injected C57BL/6 mice twice with MOG35–55 s.c. on days 0 and 19 and 300 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA) i.p. on days 0 and 2 [63]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction

All experiments were conducted under the approval of the Harvard Medical School Animal Care committee and the Animal Care. SJL/J mice were immunized subcutaneously in two sites (left and right flank) with 150 μg of PLP139-151 (New England Peptide LLC., Gardner, MA) emulsified in complete Freund’s adjuvant (CFA, Sigma Aldrich, Saint Louis, MO) containing 200 μg Mycobacterium Tuberculosis (Difco Laboratories, Detroit, MI). Mice received 200 ng pertussis toxin (PT, List Biological Laboratories Inc., Campbell, CA) in 0.2 ml PBS (Lonza, Walkersville, MD) intraperitoneally (ip) at the time of immunization and 48 h later. Control mice were immunized with CFA followed by PT (12). The mice were sacrificed at different time points. For MOG 35–55 (M-E-V-G-W-Y-R-S-P-F-S-R-O-V-H-L-Y-R-N-G-K) corresponding to mouse sequence is synthesized by QCB Inc. Division of BioSource International (Hopkinton, MA), and purified to > 99% by HPLC. Mice are immunized subcutaneously in the flanks with 150–200 μg of MOG peptide in 0.1 ml PBS and 0.1 ml CFA containing 0.4 mg Mycobacterium Tuberculosis (H37Ra, Difco, Detroit, Michigan) and injected i.p. with 200 ng Pertussis toxin (List Laboratories, Campbell, California) on the day of immunization and 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Lipopolysaccharide, Bleomycin, House Dust Mite Administration

Lipopolysaccharide (LPS, Escherichia coli O111: B4, List Biological Laboratories, Campbell, CA, USA) was administered by oropharyngeal aspiration under isoflurane anesthesia at a dose of 2.0 mg/kg of mouse body weight in 50μL of PBS. …

• Product #201 – LPS from Escherichia coli O111:B4

… Pertussis toxin List Biological Laboratories Inc 180 …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Serological B. pertussis–Specific Immunoglobulin G by ELISA

Levels of antigen-specific serum immunoglobulin (Ig) G were measured as described elsewhere [21]. Nunc 96-well plates were coated with 0.2 µg/mL pertussis toxin (PT), 0.5 µg/mL filamentous hemagglutinin adhesin, 2 µg/mL pertactin, 0.2 µg/mL fimbriae 2 and 3, or 10 µg/mL lipooligosaccharide. For adenylate cyclase toxin (ACT), Bordetella intermediate protein A (BipA), and HKBp, the plates were coated with 1 µg/mL ACT, 1 µg/mL BipA, or HKBp at an optical density of 0.9 at 600 nm. All pertussis antigens were obtained from List Biologicals. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #186 – Fimbriae 2/3 from B. pertussis

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

• Product #188L – Adenylate Cyclase Toxin, Recombinant from B. pertussis

Immunizations

8- to 12-week-old male and female mice were immunized subcutaneously with a 100 μl emulsion of 100 μg α-syn32-46 peptide (A&A peptides; San Diego, CA) dissolved in PBS with Complete Freund Adjuvant (CFA) containing 200 μg M. tuberculosis H37Ra (BD Difco Adjuvant, Fisher Scientific, Cat #DF0638-60-7; Waltham, MA). The day of immunization was designated as day 0. Mice received a boost with the same immunization dose two weeks later. Mice received intravenous injections of 400 ng of B. pertussis toxin (List Biological Laboratories, Cat #181; Campbell, CA) 14- and 16-days post-initial immunization (day 0 and 2 post boost). …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Methods
In a monocentric, randomized, double-blind, placebo-controlled, parallel-group, phase I trial, 57 healthy, smoking subjects were treated for 28 days with either oral BI 1026706 100 mg bid or placebo. At day 21, turbo-inversion recovery magnitude magnetic resonance imaging (TIRM MRI) was performed. On the last day of treatment, pre-challenge bronchoalveolar lavage fluid (BAL) and biopsies were sampled, followed by segmental LPS challenge (40 endotoxin units/kg body weight) and saline control instillation in different lung lobes. Twenty-four hours later, TIRM MRI was performed, then BAL and biopsies were collected from the challenged segments. In BAL samples, cells were differentiated for neutrophil numbers as the primary endpoint. Other endpoints included assessment of safety, biomarkers in BAL (e.g. interleukin-8 [IL-8], albumin and total protein), B1R expression in lung biopsies and TIRM score by MRI as a measure for the extent of pulmonary oedema.

… Next, Lipopolysaccharide (Endotoxin from E. coli Type O113 provided by National Institutes of Health Clinical Center, Bethesda, Maryland, USA and produced by List Biological Laboratories Inc., Campbell, California, USA) at a dose of 40 endotoxin units (E.U.)/kg …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

… To identify Vi/Vc neurons projecting to the RVM and PB, retrograde tracers Fluoro-Gold (FG; Fluorochrome) and cholera toxin subunit B (CTb; List Biological Solutions) were injected into RVM and PB in two male and two female animals. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… For active EAE induction, mice immunized with myelin peptides were additionally administered 250 ng of pertussis toxin (PTx; List Biologicals, Campbell, CA) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)