Bacterial Toxin Research Citations

Materials:

… CT was from List Biological Laboratories (Campbell, CA), …

CT intoxication assay:

CHO cells grown to 80% confluency in a 24-well plate were incubated in serum-free medium with 1, 10, or 100 ng/mL of CT for 2 hr at 37C. Toxin-challenged cells were either left untreated or were co-incubated with 50 M ribostamycin. The cAMP content of intoxicated and unintoxicated control cells was quantified with a commercial kit (Perkin-Elmer, Boston, MA) following the manufacturer’s instructions. Values obtained from unintoxicated cells were background subtracted from the results with intoxicated cells, and the experimental data were then expressed as percentages of the maximal cAMP response obtained from cells exposed to 100 ng/mL of CT in the absence of ribostamycin. Triplicate samples were used for each condition.

CT transport assay

CHO cells grown to 80% confluency in 6-well plates were pulse-labeled for 30 min at 4°C in serum-free medium containing 1 µg/mL of CT. Unbound toxin was removed by washing with PBS, after which the cells were returned to toxin- and serum-free medium for a 2 hr incubation at 37°C. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Briefly, mice (n = 60) were orally sensitized with 1 mg of OVA [28] adjuvanated with 10 µg cholera toxin (CT; List Biologicals, CA, USA) for 4 wk (twice/week), and orally treated with various glycated OVA forms (1 mg) for 4 wk (three times/week). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunocytochemistry:

Mice were deeply anesthetized with a lethal dose of anesthetic…with primary antibodies overnight at 4oC. Primary antibodies and dilutions used were:…6) goat anti-cholera toxin, -subunit (1:4000; List Biological Labs, CA);…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE:

… Animals received 200 ng of pertussis toxin (List Biological Laboratories) ip on days 0 and 2. Clinical signs of EAE were assessed according to the following scores: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Pertussis toxin (0.3 µg/200 µl, List Biological Laboratories, Campbell, USA) was administered intraperitoneally on the same day and again two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…Pertussis toxin (300 ng in PBS; List Biological, USA) was injected intraperitoneally at the time of induction and a second dose was administered three days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Vasoactive amine sensitization and challenge:

Bphs was carried out as described… Bpss and Bpbs followed a similar protocol. Briefly, 200 ng of PTX (List Biological Laboratories, Campbell, CA) in phosphate-buffered saline was administered intravenously (i.v.) by tail vein injection in a volume of 0.1 ml on day 0. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Immunization:

To induce EAE, 7-weekold female mice were injected s.c. in the flank and at the tail base with 200 µg of myelin oligodendrocyte glycoprotein 35 to 55 peptide emulsified incomplete Freunds adjuvant… Two i.p. injections of 400 ng pertussis toxin (List Biological Laboratories, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Active EAE Induction:

Active EAE was induced by immunizing 8-to12-wk-old mice…accompanied by two injections of 200 ng pertussis toxin (List Biological Laboratories),…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

For induction of PLPinduced EAE, female SJL/J (8-12 weeks) mice were immunized subcutaneously with 200µg of PLP₁₃₉₁₅₁ peptide dissolved in PBS and emulsified with an equivalent volume of CFA containing 2.5 mg/ml of Mycobacterium tuberculosis (Chondrex) under the skin at the nape of the neck and the right and left flank on day 0. Pertussis toxin (200 ng per mouse; List Biological Laboratories Inc., Campbell, CA, USA) was injected i.v. on the same day as the immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)