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3903 citations found

Functional Assay for Botulinum Neurotoxin Type A Utilizing the Neuronal Receptor Protein SV2c.

Christian, T.; Rummel, A.; Shine, N.

Product: SNAPtide® Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin

TRIF and IRF-3 Binding to the TNF Promoter Results in Macrophage TNF Dysregulation and Steatosis Induced by Chronic Ethanol

Zhao XJ, Dong Q, Bindas J, Piganelli JD, Magill A, Reiser J, Kolls JK

Product: LPS from Escherichia coli O111:B4

  • Cells and reagents:

    ... Escherichia coli LPS 0111:B4 was purchased from List Biological Laboratories, ...

    ... On day 4, the BM cells were treated with 0 or 50 mM EtOH for another 5 days. On day 9, the cell density was regulated to 1 106/ml/well in 24-well plates. After culturing overnight, medium was changed and cells were incubated for additional 6 h with 0 or 100 ng/ml LPS (E. coli 0111:B4; List Biological Laboratories). ...

    TNF- mRNA half-life assay:

    A total of 1 g/ml actinomycin was added to the culture at 0, 30, and 60 min after stimulation with LPS/PMA. Mono Mac 6 cells were collected at each time point and TNF- mRNA was measured by real-time PCR described previously.

    Chromatin IP (ChIP) assay for human IRF-3:

    Mono Mac 6 cells culture in control or EtOH conditions were plated to 24-well plate at a cell density of 2 106 of cells/ml/well, and stimulated with LPS and PMA for 1 h as stated above. Then the cells were crosslinked with 1% (v/v) formaldehyde at 37C for 20 min. ...

    Cell transduction with lentivirus and luciferase assay:

    ... BM cells were transduced overnight by lentivirus with ISRE, AP-1 driving luciferase as a reporter on day 3 before treating EtOH, and followed with EtOH and LPS as described above. After stimulation by LPS/PMA (for Mono Mac 6 cells) or LPS (for BM cells) for 6 h, the cells were harvested and ISRE, AP-1, or TNF- promoters activities were analysis using the Luciferase Reporter Assay System (Promega). The luciferase activities were normalized by total cellular proteins. ...

Capture Assay for Botulinum Neurotoxin Type A Utilizing the Neuronal Receptor Protein SV2c.

Christian, T.; Shine, N.

Product: SNAPtide® Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin

Anthrax lethal toxin induces cell death-independent permeability in zebrafish vasculature

Bolcome, RE III; Sullivan, SE; Zeller, R; Barker, AP; Collier, RJ; Chan, J

Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis

New High Affinity Antibodies Against Botulinum Neurotoxin Type A

Christian, T.; Shine, N.; Crawford, K.

Product: SNAPtide® Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin

Viral and host factors induce macrophage activation and loss of Toll Like Receptor tolerance in chronic HCV infection

Dolganiuc A, Norkina O, Kodys K, Catalano D, Bakis G, Marshall C, Mandrekar P, Szabo G

Product: Unspecified List Labs LPS

  • Reagents:

    LPS was from List Biological Laboratories (Campbell, CA) ...

    Results - Monocytes of cHCV patients lack tolerance to TNF -inducing TLR ligands:

    ... Monocytes of controls and HCV patients were primed in vitro with LPS, a TLR4 ligand, and re-stimulated with LPS to assess homotolerance. In agreement with previous reports (8,9), we found that LPS priming lead to low TNF production in response to a re-challenge with LPS in normal monocytes (Fig 1A). However, homo-tolerance to TLR4 ligand was not found in monocytes from HCV patients (Fig 1B). We further identified that pretreatment with LPS induced hetero-tolerance to subsequent stimulation with TLR2 (PGN), TLR2/TLR6 (PAM3CSK4), TLR2/TLR1 (PAM2CSK), TLR3 (poly I:C) and TLR7/8 (Gardiquimod) ligands in controls (Fig 2A), and in patients with liver inflammation due to non-viral non-alcoholic steatohepatitis (NASH) (Fig 2C) but not in HCV-infected patients (Fig 2B). This data indicated that monocytes of HCV-infected patients have lost TLR tolerance.

    Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:

A Viral Nanoparticle with Dual Function as an Anthrax Antitoxin and Vaccine

Manayani DJ, Thomas D, Dryden KA, Reddy V, Siladi ME, Marlett JM, Rainey GJ, Pique ME, Scobie HM, Yeager M, Young JA, Manchester M, Schneemann A

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

  • Quantification of PA83 bound to chimeras 206 and 264:

    Recombinant PA83 (List Biological Laboratories) in 5 mM Hepes, 50 mM NaCl (pH 7.5) was mixed with purified chimeras 206 and 264 in 50 mM Hepes (pH 7.5) in a ratio of 180:1 (equimolar amounts of PA83and VWA domains). Following incubation for 20 min at room temprature, an aliquot from each of the samples was removed and stored at 20 C pending analysis. The remainder of the samples was transferred to an ultracentrifuge tube and underlayed with a 30% (w/w) sucrose cushion in 50 mM Hepes (pH 7.5). Complexes of chimeras decorated with PA83 were pelleted by centrifugation at 200,000g for 45 min. ...

Inhibition of S. aureus a-hemolysin and B. anthracis lethal toxin by b-cyclodextrin derivatives

Karginov VA, Nestorovich EM, Schmidtmann F, Robinson TM, Yohannes A, Fahmi NE, Bezrukov SM, Hecht SM

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Activation of afferents to the ventral tegmental area in response to acute amphetamine: a double labeling study

Colussi-Mas J1, Geisler S, Zimmer L, Zahm DS, Brod A

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

  • CTb injections into the VTA:

    The retrogradely transported marker CTb (List Biological Laboratories, Campbell, CA, USA) was unilaterally injected by iontophoresis into the VTA of 16 rats. CTb is considered to be a very sensitive retrograde tracer when visualized by immunohistochemistry. ... A hand drill was used to expose the brain surface above the VTA and a borosilicate glass micropipette (tip diameter 510 m) filled with a low-salt CTb solution (0.5% made in 0.1 M phosphate buffer, pH 6.0) was lowered into the brain at the following stereotaxic coordinates: AP, 5.5 mm from bregma; L, 0.7 mm from the midline; and V 7.2 mm from the dural surface (Paxinos & Watson, 2005). CTb was delivered by applying positive pulses of 14 A (7 s on, 7 s off) for 10 min through a chlorinated silver wire placed in the micropipette and connected to a current generator (Bionic Instruments, France). The micropipette was withdrawn 10 min after the CTb ejection, the scalp incision was sutured and rats were returned to their home cages for recovery.

Induction of tolerance after establishment of peanut allergy by the food allergy herbal formula-2 is associated with up-regulation of interferon-gamma

Qu C, Srivastava K, Ko J, Zhang TF, Sampson HA, Li XM

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae