Citations

EAE:

…Pertussis toxin (List Biological Laboratories,…) was given intraperitoneally at 200 ng per mouse on the day of immunization and 48 h later. Control mice (N = 9) did not receive any treatment. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice and EAE induction:

C57BL/6 (BL/6) mice and C57BL/6 2d2 TCR-transgenic mice were generated from our in-house breeding colony… To induce EAE, …Pertussis toxin (List Biologicals; 20 ng/ ml) was injected i.v. on the day of immunization, and a second dose was administered 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of experimental autoimmune encephalomyelitis:

Mice were immunized s.c. in the anks with 300 mg mouse myelin oligodendrocyte glycoprotein peptide…and i.v. injected with 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and treatment protocol:

Each mouse also received 500 ng of PTX (pertussis toxin) (List Biological Laboratories) intraperitoneally on day 0 and day 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…The mice were also injected intraperitoneally with 800 ng Bordetella pertussis toxin (PTX, List Biological Laboratories Inc.) in 1X Phosphate Buffered Saline (PBS) at the time of, and 2 days following immunization…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Retrograde Labelling and Perfusion:

Bulbospinal neurons in the ventral medulla were retrogradely labelled with CTB (List Biological Laboratories; Campbell CA). Rats were anesthetized with isoflurane (3% for induction, 1.52% for maintenance). After removal of the spinous process of the T8 vertebra, a laminectomy was performed at T9. A small part of the rostral portion of the T10 vertebra was removed, as needed, to improve access to the spinal cord. The tip of a glass micropipette was targeted to the dorsal root entry zone (~0.51 mm lateral to midline), inserted to a depth of 1 mm and 3 60 nl of CTB was injected into each side of the cord (6 injections total). CTB injections were spaced between 0.2 and 1 mm apart in order to avoid damaging surface blood vessels. Three days after tracer injection, rats were anesthetized and perfused through the heart with DMEM/F12 tissue culture medium (D-8900; Sigma Chemical, St Louis, MO, USA) followed by 4% formaldehyde in 0.1M phosphate buffer, pH 7.4. Three untreated male Sprague-Dawley rats without CTB injections in T10 were also perfused. Brains and spinal cords in their vertebral columns were post-fixed in the same fixative for 3 days and washed in several changes of phosphate buffer. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… The animals were maintained for 6 weeks after surgery. Three days before they were sacrificed, the ipsilateral sciatic nerve was exposed at mid-thigh level and 5 µl 1% cholera toxin B subunit (CTB) (List Biological Laboratories, Campbell, CA) was slowly injected. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Immunization:

All immunization procedures were conducted in the rear footpad with 20 l injection volume per footpad. In experiments involving DT injection, 0.5 g DT (List Biological Laboratories) was injected intraperitoneally. For in vivo antigen-uptake experiments, WT B6 mice were injected in both hind legs with 2 g Alexa Fluor 488-conjugated OVA together with 50 g papain (Sigma) or CpG2216 (Invivogen).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials and Methods

Clostridium difficile Toxins. Purified TcdA (one lotlot 1-15215A1C) and TcdB (two lotslot 1-1551A1B and lot 2-15518A1B) used in this study were purchased from List Biologicals Laboratories Inc. (Campbell, CA, USA). …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

Cell culture procedures:

… Naturally immortalized keratinocytes (NIKS) were grown together with NIH 3T3 feeder cells in FAD medium, containing Ham’s F-12 medium…and 20 mg of cholera toxin (List Biological Laboratories).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae