Bacterial Toxin Research Citations

Retrograde labeling of ascending projection neurons in the brainstem:

After 2 d of survival, mice were transcardially perfused and transverse brainstem sections (20 m thickness) were prepared using a freezing microtome. For fluorescent localization, sections were incubated successively with 10% normal rabbit serum (NRS) in PBS-X containing 0.12% -carrageenan, 1% NRS, and 0.02% sodium azide (PBS-XCR) for 30 min, 1:10,000 goat anti-CTB antibody in PBS-XCR (703; List Biological Laboratories…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… CT ( Choleratoxin ) List Biological Laboratories, Inc. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Intracellular detection of LFn fusion proteins and PARP cleavage:

… Detection of toxin proteins was achieved with rabbit anti-LF and mouse anti-CdtB, both made in our laboratory, and goat anti-Pseudomonas exotoxin A (List Biological Laboratories, Campbell, CA, USA). …

• Product #760 – Anti-Exotoxin A (Goat) from Pseudomonas aeruginosa

Induction and Clinical Assessment of EAE:

…Mice were then immediately injected intravenously with 350 ng of pertussis vaccine (List Biological Laboratories, Campbell, USA) and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Intracortical tetanus and botulinum toxin injections:

For intracortical injections, a craniotomy is made over the cingulate cortex of anesthetized mice by thinning the skull with a dental drill and using a fine needle to remove a small (100 m diameter) piece of skull. Using a fine glass capillary mounted to a stereotaxic apparatus, tetanus toxin³² (25ng; 190A, List Biological Labs) or botulinum toxin (0.03ng; 128A, List Biological Labs) was diluted in 7 l of artificial cerebral spinal fluid (ACSF) and injected 500 m into the cingulate cortex.

• Product #190 – Tetanus Toxin from Clostridium tetani
• Product #128 – Botulinum Neurotoxin Type A Complex from Clostridium botulinum

… SNAPtide FITC/Dabcyl substrate, List Biological Laboratories, 521, …

• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

Experimental autoimmune encephalomyelitis (EAE):

Female C57Bl6/J mice were immunized s.c. at four sites on the flank … On the day of immunization and two days later, pertussis toxin (400 ng, List Biological Labs, CA) was administered i.p. Mice were observed for clinical signs of EAE (paralysis progressing from tail to head) for up to 30 days …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and scoring of EAE:

… Briefly, female mice (68 wk old) were induced for EAE by s.c. injection of a 200 µL IFA/PBS (v/v) solution containing 300 µg MOG peptide and 200 µg of Mycobacterium tuberculosis H37Ra (Difco) in the footpads and at the base of the limbs. Six hours later, mice were given i.v. 500 ng purified Bordetella pertussis toxin (List Biological Laboratories). A second injection of B. pertussis toxin was given after 48 h. The mice were scored daily for clinical signs of EAE as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Pertussis toxin (200 ng, List Biological Laboratories, Campbell, CA) was administrated on the day of immunization and 48 h later. For treatment of EAE, compounds 8h or vehicle PBS was orally dosed twice daily from day 0 after immunization onward. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Pertussis toxin (200 ng) (List Biological Laboratories) is also injected intravenously on day 0 and 2. Animals are observed daily for clinical signs of EAE for up to 35 days after immunization, and neurological effects were quantified on an arbitrary clinical scale…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)