Bacterial Toxin Research Citations

Materials And Methods:

… 20 ng/ml lipopolysaccharide (LPS) (List Biological Laboratories Inc., Campbell, CA, USA), …

 
HTLV-1 p30 decreases TLR4 expression:
  
… These data are consistent with the finding of Datta and colleagues (3) which
demonstrated that p30 decreases the release of pro-inflammatory cytokines, such as TNF-g, IL-8,
and MCP1 upon stimulation with the TLR4 specific agonist lipopolysaccharide (LPS) by
decreasing the expression of TLR4 itself. Similarly, we transduced THP-1 cells with p30
lentivirus or with the control lentivirus and after 12 hours of LPS stimulation, cells were
collected and analyzed by flow cytometry for intracellular production of IL-12 and TNF-g (Fig. 241
1C). In both cases, the presence of p30 decreased the expression of such cytokines upon LPS
stimulation when compared with the control.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… LPS Escherichia coli O111 : B4 LPSList Biological Laboratories, Campbell …

• Product #201 – LPS from Escherichia coli O111:B4

EAE:

Mice were immunized subcutaneously at two sites with 200 µg MOG₃₅₅₅ emulsified in Freund’s adjuvant (Sigma-Aldrich) supplemented with 5 mg/mL Mycobacterium tuberculosis (strain H37Ra, Difco). On the day of and 2 d after immunization, 400 ng pertussis toxin (List Biological Laboratories) was administered i.p. EAE severity was assessed every day based on a clinical scoring system …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animals:

C57BL/6 mice (6-8 weeks old) were purchased …Animals were immunized subcutaneously in both sides of the flank with a total dose of 100µg MOG:3555 (EZBiolab, Carmel, IN, USA) emulsified in CFA (injection volume=200 µl). Each mouse received 200ng pertussis toxin (List Biological Laboratories, Hornby, Ontario, Canada) in 500µl sterile phosphate-buffered saline (PBS) on the day of immunization and 48h later. Clinical symptoms were evaluated daily according to the standard EAE scale: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and scoring:

EAE was induced essentially as described previously ²⁵. Briefly, mice were injected s.c. with an emulsion containing 100 µg of MOG_35-55 peptide…Alternatively, mice were immunized with 200 µg of MOG_35-55 in CFA, followed by i.v. administration of 200 ng pertussis toxin (List Biological) (1 MOG_35-55/CFA/PTX).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

200 ng pertussis toxin (List Biological Inc.) were injected i.p. on day 0 and day 1.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To deplete dendritic cells, CD11c-DTR mice were treated i.v. with 100ng diphtheria toxin in PBS (List Biological Laboratories) one day prior to SMARTA cell transfer. A second treatment was given one day after cell transfer. PBS vehicle was injected into undepleted controls.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents and cell culture:

Staphylococcus aureus enterotoxin B (SEB) was obtained from List Biological Laboratories INC. (Campbell, CA, USA) …

Anatomical tracer injections:

…The injection micropipette (tip ca. 15 m) was filled with cholera toxin subunit b (CTb; 1%, List Biological, Campbell, CA, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cell culture and transfection:

DMEM/Hams F12 medium supplemented with… and 100 ng/ml cholera toxin (List Biological Labs). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae