Citations

Experimental Autoimmune Encephalomyelitis:

Female mice were immunized by subcutaneous administration with 200 m g of MOG 3555 peptide (Invitrogen) emulsified in IFA (Difco) containing 500 m g of TDM on day 0, supplemented with intravenous injection of 200 ng of pertussis toxin (List Biological Laboratories). Mice received an additional intraperitoneal injection of 200 ng pertussis toxin on day 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…For ELISA, 50 L of a 5 g/mL solution of anthrax PA, activated PA63 (List Biological Labs, Campbell, CA, USA), was dissolved in carbonate-bicarbonate buffer and used to coat each well of a 96-well plate …’

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

… The following primary antibodies are recommended: goat antibody against CTB (1:1,000, No. 703; List Biological Laboratories, Campbell, CA, USA) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Cell Culture, Proteins, Antibodies and Chemicals:

…Cholera toxin was obtained from List Biological Laboratory.

Immunofluorescence Staining and Confocal Laser Scanning Microscopy:

…Cells without CARDS toxin were processed similarly to serve as negative controls. In parallel, cells treated with cholera toxin (0.5 µg) or Tf (10 µg), plus their respective antibodies at 1:500 dilution, served as positive controls for caveolin-mediated and clathrin-mediated endocytosis.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunization EAE:

Mice were immunized subcutaneously at the base of the tail with 50 g MOG₃₅₅₅ peptide (Schafer-N, Copenhagen, Denmark) in PBS emulsified in complete Freunds adjuvant (CFA). To induce the development of EAE, MOG-peptide immunized C57Bl/B6 mice were also injected twice (on the same day and two days later) with Pertussis toxin (200 ng; List Biological Laboratories, Inc. Campbell, Ca) in PBS.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… CT was purchased from List Biological Laboratories (Campbell, Calif.). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Vaccines and adjuvants:

…The preparation and characterization of vaccines and dmLT are described in the Appendix; CT was purchased from List Biological Laboratories (Campbell, CA, USA).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Hormone treatment and induction of EAE:

Female WT, PD-L1^/ and PD-L2^/ mice were implanted with 2.5 mg/60-day release 17-estradiol pellets (Innovative Research of America, Sarasota, FL) or sham-treated (control) one week prior to immunization with 200 g mouse (m) MOG_35-55 peptide (PolyPeptide Laboratories, San Diego, CA) in 400 g Complete Freunds adjuvant (CFA, H37Ra, Difco, Detroit, MI). Mice received pertussis toxin (Ptx, List Biologicals, Campbell, CA) on the day of immunization (75 ng) and 2 days later (200 ng). All mice were monitored daily for clinical signs of disease and scored using the following scale: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials and Methods – Reagents:

LPS was obtained from List Biological Laboratory …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Cell Culture:

C3H10T1/2 cells (ATCC) were cultured in BME (Gibco) with 10% fetal bovine serum (PAA Laboratories) and penicillin-streptomycin. Cells were grown at 37C and 5% CO₂. For qPCR analysis, 5.010⁴ cells were plated in a 6-well dish. For protein extracts, 1.010⁶ cells were plated in a 15 cm plate. Where indicated, cells were treated with Shh (1 g/mL; R&D Systems) for 2 hours, 6 hours, or 24 hours. Where indicated, cells were treated for 24 hours with SAG (100 nM; Santa Cruz) or pertussis toxin (PTX; 100 ng/mL; List Biological Laboratories Inc.).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)