Citations

Induction of experimental autoimmune encephalomyelitis

…Mice also received 200 ng pertussis toxin in 200 µl PBS (List Biological Laboratories, Campbell, CA) i.p. on the day of priming and two days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials and Methods:

…To ablate macrophages in Itgam-Dtr transgenic mice or lysozyme M Cre Dtr fl/fl mice, 20 ng/g body weight diphtheria toxin (List Biological Laboratories, Campbell, CA) was injected intraperitoneally at 1 day before laser induction and 1 day after laser injury. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… After anesthesia with a mixture of ketamine (62.5 mg/kg ip) and xylazine (12.5 mg/kg ip), animals had 1% cholera toxin subunit B (CTB; List Biologicals, Campbell, CA; freshly diluted in sterile distilled water) injected into the vitreous chamber of the right eye. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… In addition, gross observations of changes in behavior (catatonia, agitation, ataxia, sedation), as well as levels of alertness and orientation to a cotton applicator were recorded throughout the period of testing. PTX (List Biologicals, Campbell, CA) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Retrograde Labeling of Phrenic Motoneurons:

Intrapleural injection of cholera toxin subunit B (CTB) was used to retrogradely label phrenic motoneurons in immunohistochemical and laser capture microdissection experiments. This technique was previously validated for this purpose and labels only phrenic motoneurons in the cervical spinal cord [28]. Briefly, 20 µl of a 0.2% solution of either Alexa Fluor 488-conjugated CTB (Molecular Probes; Life Technologies, Grand Island, NY) or unconjugated CTB (List Biological Laboratories, Campbell, CA) was injected using a Hamilton syringe into the pleural space of the right and left sides of the chest between the 7th and 8th ribs at 3 days before the terminal experiment.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Reagents:

… Ultrapure LPS (Escherichia coli 0111:B4) was purchased from List Biological Laboratories (Vandell Way, CA, USA). …

Sucrose Gradient Fractionation:

RAW264.7 cells were grown on 10-cm tissue culture plates and stimulated with LPS or HMGB1 for the indicated times.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE Induction:

EAE was induced 23 weeks following transfer of memory cells. Recipient mice or untransferred controls were immunized…in four sites on the back and received 100 ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) intraperitoneally on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Adoptive transfer of T-cell subsets or whole lymphocytes:

Treatment with pertussis toxin (PTX) was performed by incubating 4 10⁷ cells/mL in RPMI containing 0.1M N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid and 2% fetal calf serum with 100 ng/mL PTX (List Biological Laboratories, Campbell, CA) for 2 hours at 37C, and washed >4 times.  Donor cells were labeled with 5 M of either carboxyfluorescein diacetate succinimidyl ester…and intravenously injected into host mice.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Two intraperitoneal injections of 250 ng pertussis toxin (List Biologicals, Campbell, CA) were administered on days 0 and 2. Mice were observed and scored according to disease severity, where a score of 0 = no disease, 1 = limp tail, 2 = hind-limb paresis, 3 = hind-limb …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Functional Characterization of Peritoneal and Bone Marrow-derived Macrophages:

Peritoneal macrophages (p-m) were harvested as described above. …For experiments macrophages were cultured at a density of 0.510⁶ cells/mL and were incubated for 24 hours in the absence or presence of lipopolysaccharide (LPS; 50 ng/mL; Salmonella Minnesota R595 (Re); List Biological Laboratories Inc. Campbell, CA)…

• Product #304 – LPS from Salmonella minnesota R595 (Re)