Bacterial Toxin Research Citations

EAE:

Mice were immunized subcutaneously at two sites with 200 µg MOG₃₅₅₅ emulsified in Freund’s adjuvant (Sigma-Aldrich) supplemented with 5 mg/mL Mycobacterium tuberculosis (strain H37Ra, Difco). On the day of and 2 d after immunization, 400 ng pertussis toxin (List Biological Laboratories) was administered i.p. EAE severity was assessed every day based on a clinical scoring system …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animals:

C57BL/6 mice (6-8 weeks old) were purchased …Animals were immunized subcutaneously in both sides of the flank with a total dose of 100µg MOG:3555 (EZBiolab, Carmel, IN, USA) emulsified in CFA (injection volume=200 µl). Each mouse received 200ng pertussis toxin (List Biological Laboratories, Hornby, Ontario, Canada) in 500µl sterile phosphate-buffered saline (PBS) on the day of immunization and 48h later. Clinical symptoms were evaluated daily according to the standard EAE scale: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and scoring:

EAE was induced essentially as described previously ²⁵. Briefly, mice were injected s.c. with an emulsion containing 100 µg of MOG_35-55 peptide…Alternatively, mice were immunized with 200 µg of MOG_35-55 in CFA, followed by i.v. administration of 200 ng pertussis toxin (List Biological) (1 MOG_35-55/CFA/PTX).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

200 ng pertussis toxin (List Biological Inc.) were injected i.p. on day 0 and day 1.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To deplete dendritic cells, CD11c-DTR mice were treated i.v. with 100ng diphtheria toxin in PBS (List Biological Laboratories) one day prior to SMARTA cell transfer. A second treatment was given one day after cell transfer. PBS vehicle was injected into undepleted controls.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents and cell culture:

Staphylococcus aureus enterotoxin B (SEB) was obtained from List Biological Laboratories INC. (Campbell, CA, USA) …

Anatomical tracer injections:

…The injection micropipette (tip ca. 15 m) was filled with cholera toxin subunit b (CTb; 1%, List Biological, Campbell, CA, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cell culture and transfection:

DMEM/Hams F12 medium supplemented with… and 100 ng/ml cholera toxin (List Biological Labs). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Screening Against LF:

… Each well contained a volume of 90 L including buffer (20 mM HEPES, pH 7.4), recombinant LF (10 nM final concentration, List Biological Laboratories), and the fragment solution (1 mM final concentration). After a 20 min incubation period at 25 C, the reaction was initiated by the addition of 10 L fluorogenic LF substrate (2 M final concentration, MAPKKide DABCYL/FITC, List Biological Laboratories).

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

EAE Induction:

… Mycobacterium tuberculosis (MTB) H37Ra (Difco). At the time of EAE induction and 48 h later, mice were injected intraperitoneally with 200 ng pertussis toxin (List Biologicals) in 200 µL of PBS. Following the encephalitogenic challenge …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)