Bacterial Toxin Research Citations

Animals:

C57BL/6 mice (6-8 weeks old) were purchased …Animals were immunized subcutaneously in both sides of the flank with a total dose of 100µg MOG:3555 (EZBiolab, Carmel, IN, USA) emulsified in CFA (injection volume=200 µl). Each mouse received 200ng pertussis toxin (List Biological Laboratories, Hornby, Ontario, Canada) in 500µl sterile phosphate-buffered saline (PBS) on the day of immunization and 48h later. Clinical symptoms were evaluated daily according to the standard EAE scale: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and scoring:

EAE was induced essentially as described previously ²⁵. Briefly, mice were injected s.c. with an emulsion containing 100 µg of MOG_35-55 peptide…Alternatively, mice were immunized with 200 µg of MOG_35-55 in CFA, followed by i.v. administration of 200 ng pertussis toxin (List Biological) (1 MOG_35-55/CFA/PTX).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

200 ng pertussis toxin (List Biological Inc.) were injected i.p. on day 0 and day 1.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To deplete dendritic cells, CD11c-DTR mice were treated i.v. with 100ng diphtheria toxin in PBS (List Biological Laboratories) one day prior to SMARTA cell transfer. A second treatment was given one day after cell transfer. PBS vehicle was injected into undepleted controls.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents and cell culture:

Staphylococcus aureus enterotoxin B (SEB) was obtained from List Biological Laboratories INC. (Campbell, CA, USA) …

Anatomical tracer injections:

…The injection micropipette (tip ca. 15 m) was filled with cholera toxin subunit b (CTb; 1%, List Biological, Campbell, CA, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cell culture and transfection:

DMEM/Hams F12 medium supplemented with… and 100 ng/ml cholera toxin (List Biological Labs). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Screening Against LF:

… Each well contained a volume of 90 L including buffer (20 mM HEPES, pH 7.4), recombinant LF (10 nM final concentration, List Biological Laboratories), and the fragment solution (1 mM final concentration). After a 20 min incubation period at 25 C, the reaction was initiated by the addition of 10 L fluorogenic LF substrate (2 M final concentration, MAPKKide DABCYL/FITC, List Biological Laboratories).

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

EAE Induction:

… Mycobacterium tuberculosis (MTB) H37Ra (Difco). At the time of EAE induction and 48 h later, mice were injected intraperitoneally with 200 ng pertussis toxin (List Biologicals) in 200 µL of PBS. Following the encephalitogenic challenge …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Injection of retrograde tracer:

CTb (2%, List Biological Laboratories, Campbell, CA, USA), dissolved in distilled water, was targeted into the right dlPAG (coordinates: 6.36.6 mm posterior to Bregma, 1.6 mm lateral to the midline, 4.44.9 mm from the surface of the dura mater, angle 15) of 13 animals. The tracer was delivered into the targeted area through a glass micropipette, either by pressure injection (pipette tip diameter 2030 m; volume ranged between 20 and 75 nl) using an automated air-pressure…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt