Bacterial Toxin Research Citations

… EAE was induced by injecting the mice subcutaneously into the flanks with 200 µl of emulsion containing 200 g MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK) and 500 µg M. tuberculosis H37 Ra (Difco) in incomplete Freund Adjuvant oil (Difco). In addition, the mice received 400 ng pertussis toxin (List Biological Laboratories) intraperitoneally (i.p.) on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…Active CD4+ T cellmediated EAE was induce d by immunizing mice with 100 µg of rMOG emulsifie d in complete Freunds adjuvant (CFA) containing 1 mg/ml mycobacteria (Sigma) accompanied by two injections of 200 ng pertussis toxin (List Biological Laboratories) as described …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In vitro migration and actin polymerization:

Migration assays were performed using 24-well transwell plates with 5 m pore size filter inserts (Corning). Prior to migration assays, L1.2 or THP-1 cells were pretreated with either 100 M BX-471 (kind gift of Richard Horuk, UC Davis, Davis, CA), 0.2 µg/mL pertussis toxin (List Biological Laboratories) or DMSO as a control for 1 h at 37°C. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and evaluation of EAU:

Experimental autoimmune uveitis was induced in DAP12 null mice and in WT C57BL/6 controls housed within the same room of the same facility by immunization with whole bovine IRBP, at 150µg/mouse, and IRBP 1-20 peptide, at 200µg/mouse, emulsified in complete Freund’s adjuvant. In addition, the mice were treated intraperitoneally with 0-15µg pertussis toxin (List Biological Laboratories, Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Moreover, 200 ng pertussis toxin (PTx; List Biological Laboratories, …) were administered in 500 µl sterile phosphate-buffered saline (PBS) on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.3. Experimental autoimmune encephalomyelitis:

Mice were immunized subcutaneously in two locations on the back with 20 or 100 µg of MOG_35-55 peptide in incomplete Freunds Adjuvant (Sigma-Aldrich, St. Louis, MO) complemented with0.5mg/ml of Mycobacterium tuberculosis H37RA… In addition, 200 ng of pertussis toxin (List Biological Laboratories, …) was administered i.p. immediately following immunization and again 48 hours later. Clinical assessment of EAE was performed daily according to the following scoring system: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… subcutaneously with 300 µg of MOG 3555 emulsified with an equal volume of complete Freund’s adjuvant…and then injected with 400 ng of pertussis toxin (List Biological Laboratories, USA …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pertussis toxin (PTx) facilitates the movement of autoreactive T cells to brain tissues to induce EAE in mice⁴¹. Treatment of DKO mice with PTx led to a rapid wasting disease with death starting in one month (data not shown). Consistently, there were increased lymphocytic infiltrates into organs accompanied by robust effector cytokine production by CD4⁺ T cells

Pertussis toxin was from List Biological Laboratories, Campbell CA.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Bacterial strains, toxin, and antibodies:

…Purified V. cholerae type Inaba 569B (catalog no. 100B), heat-labile (catalog no. 165B), Shiga (catalog no. 161), and diphtheria (catalog no. 150) toxins were purchased from List Biological Laboratories (Campbell, CA). …

List Catalog #165B has been discontinued.

RTCA system:

…The toxin concentration for a sample was quantified using a calibration curve as described previously, with minor modifications (36). In brief, a series of 2-fold dilutions (from 3.5 pg/ml to 1.8 ng/ml) of purified CT were made in DPBS with 0.1% BSA. …

Cell morphological analysis:

The Y-1 cells were viewed at a time point at which a significant CI change was detected during the continuous monitoring by the RTCA system. The real-time status of the Y-1 cells in the 96-well microplate incubated with purified CT was monitored with an inverted phase-contrast microscope and photographed with an integrated digital camera…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #150 – Diphtheria Toxin, Unnicked, fromCorynebacterium diphtheriae
• Product #161 – Shiga Toxin 1 from Escherichia coli

Adult ablation of MCH neurons improves glucose tolerance, even in absence of MCH signaling:

MCH neurons increase their firing rate in situ when extracellular glucose increases (Burdakov et
al., 2005). Ten days after DT injection, MCH-neuron-ablated mice showed reduced area-under
the-curve (AUC) during a glucose tolerance test … Hypothesizing that this phenotype of MCH-neuron
ablation resulted from the loss of a non-MCH neurotransmitter in MCH neurons, I measured
glucose tolerance in homozygous PmchDTR/DTR mice injected with DT.

… Some vendors have proven more reliable than others at producing DT that is free of side effects in wild-type mice. We have had good luck with DT from List Biological Laboratories, but problems with that obtained from Sigma-Aldrich. DT comes lyophilized in vials of 1 mg. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae