Bacterial Toxin Research Citations

Reagents:

LPS (catalog no. 421) was purchased from List Biological Laboratories (Campbell, CA). 

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

HGF expression and secretion from myofibroblasts:

Myofibroblasts from three to four independent primary myofibroblast cultures isolated from intestines of Apc^min/+ or Apc^min/+/Epim^/ mice were plated (3 10⁵ cells per well in 6-well plates) and grown for 48 h in myofibroblast culture media containing FBS. Media was removed and replaced with serum-free (SF) myofibroblast media, SF myofibroblast media containing LPS (5 g/ml; List Biological Laboratories, Campbell, CA), …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… In the experiments using the inhibitors, cells were pretreated, alone or in combination, with either vehicle (water) or 100 ng/ml pertussis toxin (PTX) (List Biological Laboratories, Campbell …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… On the day of immunization and again two days later, each mouse was injected ip with 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA). EAE severity was assessed daily as …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and Clinical Evaluation:

For the PLP-induced EAE, SJL/J female mice (5-7 weeks old) were immunized subcutaneously (s.c.) with 200 µg of PLP139-151 peptide in a 0.2 ml emulsion comprised of equal volumes of phosphate-buffered saline (PBS) solution and CFA containing killed mycobacterium tuberculosis strain H37RA at a final concentration of 4 mg/ml (Difco, Detroit, MI). The PLP/CFA emulsion was administered to regions above the shoulder and the flanks (total of 4 sites; 50 µl at each injection site). In addition, 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) was injected intraperitoneally (i.p.) on the day of immunization (day 0) and 48 h post-immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization procedures:

To measure recall responses, peptides were emulsified in complete Freunds adjuvant (CFA) and administered s.c. (50 g/mouse) in the inguinal and sternal regions and foot-pads (Massilamany et al., 2011a, 2011b). For disease induction, peptides were emulsified in CFA supplemented with Mycobacterium tuberculosis H37RA extract (Difco Laboratories, Detroit, MI) to a final concentration of 5 mg/ml, and the emulsions were administered at indicated doses s.c. in the inguinal and sternal regions and base of the tail. Additionally, pertussis toxin (PT; List Biological Laboratories, Campbell, CA) was administered i.p. at indicated doses on day 0 and day 2 postimmunization ( Silver et al., 1995; Namba et al., 1998).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell transfers:

… For BALB/c donors, cells were incubated with CellTrace Violet… In some experiments, cells were incubated with 200 ng/ml pertussis toxin (List Biological Laboratories) for 1 h before transfer. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Multisynaptic tracing using PRV:

Rats were anesthetized by isoflurane inhalation (Halocarbon Laboratories, River Edge, NJ; 13% in oxygen) and positioned into a Kopf stereotaxic device. A pulled glass pipette tip was attached to the stereotaxic arm and the back end of the glass pipette was affixed to a polyethylene tube connected to a PicoPump (World Precision Instruments, Sarasota, FL). The glass pipette was back-filled with solution containing PRV-263 (3.4 10⁸ pfu / mL) or a 3:1 mixture of PRV-263 and a 0.25% solution of cholera toxin subunit B (CTB; List Biological Labs, Campbell, CA, USA) diluted in deionized water. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Distribution of retrogradelly labeled cells:

…For injections to the 3V, the tissue was rinsed (6 times, 10 min/rinse) in 0.05 M tris-buffered saline (TBS) in 0.04% TX, blocked (30 min) in 5% normal rabbit serum (S-5000; Vector Laboratories) in 0.04% TX-TBS, and rinsed once in 0.04% TX-TBS. Then, sections were incubated in a goat anti-CTB antibody (1:5000 in 0.04% TX-TBS; 703; List Biological Laboratories, Inc., Campbell, CA, USA) for 60 h at 4C. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

LT treatment of NRVM:

Approximately 24 h after NRVM plating, NRVM were treated with LT in a 2:1 ratio of PA:LF prepared in dispersion medium. PA (activated, 63 kD segment) and LF were purchased from List Biological Laboratories (Campbell, CA) and each reconstituted in glycerol at a concentration of 0.5 mg/mL.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis