Bacterial Toxin Research Citations

ELISA assays:

For stimulation experiments, cells were grown in 48 well plates for 4 days and the indicated stimulus was added in triplicates to the cell medium. E. coli Dm31 LPS was purchased from List Biological Laboratories (Denver, CO), …

• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)

Endotoxemia:

Ultra Pure LPS (Escherichia coli 0111:B4) from LIST Biologicals (Campbell, CA) was dissolved in sterile normal saline and injected intraperitoneally (1.5 mg/kg). At various time points after LPS, mice were euthanized, blood was isolated by cardiac puncture, and the kidneys were harvested.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE:

… For active EAE induction, mice were immunized in two sites on the hind flanks with 300 µg MOG35-55 in 100 µl CFA containing 5 mg/ml heat-killed M. tuberculosis strain H37Ra (Difco) at day 0 and day 7. Pertussis toxin (List Bio Lab) was administrated intraperitoneally at the dosage of 500 ng per mouse at day 1 and day 8. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

LPS from Escherichia coli O111:B4 was from List Biological Laboratories (Campbell, CA). …

In Vitro Generation and Activation of BMDCs:

BMDCs were generated from gal3^+/+ and gal3^/ mice by culturing bone marrow cells for 8 to 10 days as described… For measuring cytokine production, 2 10⁵ DCs per well were stimulated with 0.1 or 100 ng/mL of LPS; 1, 10, or 100 g/mL of curdlan; or 10⁵, 10⁶, 10⁷ cells/mL of HKCA in 96-well plates at 200 L per well for 24 hours.

• Product #201 – LPS from Escherichia coli O111:B4

Reagents:

Lipopolysaccharide (LPS) was obtained from List Biological Laboratories (San Jose, CA, USA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Cell culture, construction of EST library, and screening of EST libraries with TcdB:

…To identify clones that are less sensitive to TcdB killing, cultures containing 2 10⁶ HeLa-tTA EST library cells or a similar number of HeLa-tTA naive cells were plated in a 150-mm culture dish and treated with 2 ng/ml TcdB (List Biological Laboratories, Inc., Campbell, CA). …

• Product #155 – Toxin B from Clostridium difficile

Immunization and challenge

One-week-old neonatal BALB/c mice were immunized with five different substances via intranasal (i.n.) or footpad routes: live RSV A2 (5×104 PFU), Gcf (20 μg), Gcf (20 μg) with cholera toxin (CT; List Biological Laboratories, Campbell, CA) adjuvant (1 μg), and PBS were administered via the i.n. route whereas FI RSV A2 (1×105 PFU) was injected into mouse footpads. Two weeks later, mice were primed with the same antigen and adjuvant. At 4 to 5 weeks after the last immunization, all mice were challenged with live RSV A2 (1×106 PFU).

To test the maternal effect of antigen exposure before pregnancy, female BALB/c mice were i.n. infected twice with RSV A2 (1×105 PFU) or PBS at 2-week intervals, and mated 4 weeks after the last infection to obtain offspring. When their neonates were 1 week old, the mice were divided into two subgroups and immunized: one group was immunized i.n. with Gcf (20 μg) plus CT adjuvant (1 μg) twice at 2-week intervals and the other group (the control) was immunized with PBS alone. Five weeks after the final immunization, mice were challenged with live RSV A2 (1×106 PFU). At 4 days after challenge, the eosinophil population in bronchoalveolar lavage (BAL) fluid was analyzed by flow cytometry, and a plaque assay was performed using lung homogenates.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

…Cholera toxin (CT) was purchased from List Biological Laboratories, Inc. (CA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Endothelial cell permeability assay:

Human microvascular endothelial cells (HMEC; Biological Products Branch, Centers for Disease Control, Atlanta, GA) were incubated at 37C and 5% CO₂ and cultured as recommended.¹⁹ Macromolecule permeability was measured as previously described²⁰ with slight modifications. …Inserts were placed in Ca²⁺-containing Hanks buffered saline solution with or without LPS (10g/ml, List Laboratories; Campbell CA), hAAT (0.5mg/ml) or both. Following either 6 or 24 h LPS treatment, FITC-labeled dextran 70kD…

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Chemicals and reagents:

Lipopolysaccharide (LPS; from Escherichia coli 0111:B4) was obtained from List Biological Laboratories, Inc.

Cell culture and treatments:

The human lung adenocarcinoma epithelial cell line A549…The cells were treated with or without LPS (1 g/ml) and cytokine mixture (TNF-, IFN-, and IL-1, 20 ng/ml each) for up to 24 h before harvest of the conditioned media and cells for nitrite measurement and Western blotting…

• Product #201 – LPS from Escherichia coli O111:B4