Bacterial Toxin Research Citations

Induction and Clinical Evaluation of EAE:

EAE was induced in 8-week-old female SJL/J mice (Oriental Yeast Corp., Tokyo, Japan) by subcutaneous injection of 150 µg of PLP₁₃₉₁₅₁ (Biologica, Nagoya, Japan) peptide emulsified in complete Freunds adjuvant …In non-obese diabetic (NOD)/ShiJcl mice, EAE was induced by immunization with MOG₃₅₅₅ peptide (Biologica) emulsified in CFA at a dose of 200 µg, followed by the administration of pertussis toxin (200 ng; List Biological Laboratories Inc., Colorado, USA) on days 0 and 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pharmacological Agents:

… pertussis toxin (PTX; G i and G o protein inhibitor) [500 mg/liter] was from List Biologicals (Campbell, CA), …

Implication of a Surface-localized GPCR:

The engagement of PLC-PKC and PKA pathways by CORT in activating Pyk2 hinted at the potential signaling role of G proteins. Consistent with previous demonstrations that the PLC-PKC pathway can be activated by PTX-insensitive G_q subunits as well as PTX-sensitive G/_i subunits (34), we observed here that pretreatment with PTX prevents CORT-triggered phosphorylation of Pyk2 and Src (Fig. 6A); these findings implicate the involvement of the – subunits of G_i (34). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cellular assays:

…To test the capacity of DCs to mature after stimulation, DCs were incubated in the presence of Ultra Pure LPS (List Biological Labs) 10 ng/ml or media overnight. Expression of costimulatory markers was assessed by ow cytometry and fold induction for each marker was calculated as MFILPS/MFIMedia. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE induction:

Mice were injected i.p. with pertussis toxin (400 ng/dose; List Biological Labs) on days 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… On day 0, each mouse was first given 200 ng pertussis toxin (List Biological Laboratories, Campbell, Calif., USA) in 200 µl PBS intraperitoneally (ip). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Enzyme-linked immunosorbent assay (ELISA):

A 50 L of a 5 g/mL solution of activated PA63 (List Biological Labs, Campbell, CA, USA) dissolved in 50 mM carbonate-bicarbonate buffer (pH 9.6) (Sigma-Aldrich) was used to coat each well of a 96-well transparent flat plate …

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Reagents:

…Recombinant forms of Bacillus anthracis PA63, LF, and EF were obtained from List Biological Laboratories (Campbell, CA) and reconstituted per the manufacturer’s instructions. …

The following applications were performed using the above listed Anthrax products:

• Integration of recombinant PA₆₃ artificial membranes
• The pH dependence of PA₆₃ conductance and LF binding
• Effect of EF/ LF present during acidification
• Planar membrane rupture by recombinant proteins
• Planar membrane rupture by cationic polymers
• Planar membrane rupture by animal isolates
• Droplet membrane rupture by animal isolates
• Molecular modeling

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

 Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

ELISA assays:

For stimulation experiments, cells were grown in 48 well plates for 4 days and the indicated stimulus was added in triplicates to the cell medium. E. coli Dm31 LPS was purchased from List Biological Laboratories (Denver, CO), …

• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)

Endotoxemia:

Ultra Pure LPS (Escherichia coli 0111:B4) from LIST Biologicals (Campbell, CA) was dissolved in sterile normal saline and injected intraperitoneally (1.5 mg/kg). At various time points after LPS, mice were euthanized, blood was isolated by cardiac puncture, and the kidneys were harvested.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE:

… For active EAE induction, mice were immunized in two sites on the hind flanks with 300 µg MOG35-55 in 100 µl CFA containing 5 mg/ml heat-killed M. tuberculosis strain H37Ra (Difco) at day 0 and day 7. Pertussis toxin (List Bio Lab) was administrated intraperitoneally at the dosage of 500 ng per mouse at day 1 and day 8. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)