Bacterial Toxin Research Citations

Vaccines and adjuvants:

…The preparation and characterization of vaccines and dmLT are described in the Appendix; CT was purchased from List Biological Laboratories (Campbell, CA, USA).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Hormone treatment and induction of EAE:

Female WT, PD-L1^/ and PD-L2^/ mice were implanted with 2.5 mg/60-day release 17-estradiol pellets (Innovative Research of America, Sarasota, FL) or sham-treated (control) one week prior to immunization with 200 g mouse (m) MOG_35-55 peptide (PolyPeptide Laboratories, San Diego, CA) in 400 g Complete Freunds adjuvant (CFA, H37Ra, Difco, Detroit, MI). Mice received pertussis toxin (Ptx, List Biologicals, Campbell, CA) on the day of immunization (75 ng) and 2 days later (200 ng). All mice were monitored daily for clinical signs of disease and scored using the following scale: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials and Methods – Reagents:

LPS was obtained from List Biological Laboratory …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Cell Culture:

C3H10T1/2 cells (ATCC) were cultured in BME (Gibco) with 10% fetal bovine serum (PAA Laboratories) and penicillin-streptomycin. Cells were grown at 37C and 5% CO₂. For qPCR analysis, 5.010⁴ cells were plated in a 6-well dish. For protein extracts, 1.010⁶ cells were plated in a 15 cm plate. Where indicated, cells were treated with Shh (1 g/mL; R&D Systems) for 2 hours, 6 hours, or 24 hours. Where indicated, cells were treated for 24 hours with SAG (100 nM; Santa Cruz) or pertussis toxin (PTX; 100 ng/mL; List Biological Laboratories Inc.).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Slice preparation and electrophysiology (in rats):

In indicated experiments KCl was reduced to 4 mM or the following were added: 10 mM 1,2-Bis(2-aminophenoxy)ethane-N,N,N,N-tetraacetic acid (BAPTA; Sigma), 5gmL¹ Botulinum Neurotoxin Type C (Light Chain Recombinant BoNT/C; List Biological),

• Product #625A – Botulinum Neurotoxin Type C Light Chain, Recombinant

Slice Preparation and Electrophysiology (in mice):

…the light chain BoNT/C (the proteinase subunit which lacks membrane permeability, 5 g/ml, List Biological Laboratories) were included in the internal solution.

• Product #625A – Botulinum Neurotoxin Type C Light Chain, Recombinant

… A commercially available rLF used as control was purchased from List Biological Laboratories. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

… Animals were immunized via the intranasal route with 5 µg cholera toxin (CT; List Biological Laboratories, Campbell, CA, USA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunization:

Once weekly for 3 consecutive weeks, female BALB/c mice were immunized intranasally with cCHP-PspA, PspA plus CT (1 µg; List Biological Laboratory, Campbell, CA), PspA alone, or phosphate-buffered saline (PBS) only. Some experiments included an irrelevant antigen as a control; …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Table 1. Antibody Characterization:

Cholera toxin subunit B, Purified CTB isolated from Vibrio cholerae, List Biological Laboratories (Campbell, CA), goat polyclonal #703, 1:5,000. …

Histology:

Table 1 lists all antibodies used. The CTB antibody was tested on galago brain tissue with no CTB injections and this control failed to label any cells or patches of axon terminals.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)